In Vivo Evaluation of Platelet Activation by Different Cellulosic Membranes

Cases, Aleix; Reverter, Joan Carles; Escolar, Ginés; Saw, C. B.; Sorribes, Josep; Ordinas, A

doi:10.1111/j.1525-1594.1997.tb00371.x

Cited by 16 publications

(3 citation statements)

References 16 publications

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“…Complement activation and platelet–neutrophil coaggregate formation are interrelated, since C5a and C3a, generated by complement activation, induced neutrophils to express adhesion molecules and to promote platelet–neutrophil adhesion. Several reports have in fact shown that RC membranes induce the activation of platelets during hemodialysis 32–34. The platelet activation by these membranes is possibly mediated by intricate processes involving complements and leukocytes.…”

Section: Discussionmentioning

confidence: 99%

Platelet activation through interaction with hemodialysis membranes induces neutrophils to produce reactive oxygen species

Itoh

Susuki

Tsuji

2006

J Biomedical Materials Res

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The intradialytic activation of leukocytes is one of the major causes of hemodialysis-associated complications. During hemodialysis, the formation of microaggregates consisting of platelets and neutrophils has been observed to accompany the production of reactive oxygen species (ROS) by leukocytes. In this study, we investigated the interaction of platelets and neutrophils with hemodialysis membranes in vitro to elucidate the mechanism underlying microaggregate formation and its relevance to leukocyte activation. The production of ROS in neutrophils was induced by the coincubation of neutrophils with polysulfone (PS) membranes, and was increased when platelets were present in the neutrophil suspension. Neutrophils that were incubated with polymethylmethacrylate (PMMA) membranes in the presence of platelets also produced significant levels of ROS, suggesting that the presence of platelets augmented ROS production in neutrophils. Platelets adhered more firmly to hydrophobic membranes such as PS and PMMA membranes than to hydrophilic membranes, such as those composed of regenerated cellulose (RC) or ethylene vinylalcohol copolymer (EVAL). The adhesion of platelets to dialysis membranes composed of different materials was correlated with those membranes' ability to induce platelet activation as assessed by the cell surface expression of P-selectin. Moreover, coincubation of neutrophils with platelets that had been treated with hydrophobic membranes induced a higher level of superoxide anion relative to those treated with hydrophilic membranes in association with the P-selectin-mediated microaggregate formation. These results suggest that platelets activated through interaction with hemodialysis membranes stimulate neutrophils to produce ROS via P-selectin-mediated adhesion, and that this property of adhesion to platelets is critical for the biocompatibility of hemodialysis membranes.

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Section: Discussionmentioning

confidence: 99%

Platelet activation through interaction with hemodialysis membranes induces neutrophils to produce reactive oxygen species

Itoh

Susuki

Tsuji

2006

J Biomedical Materials Res

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“…Активация каскада свертывания крови и тромбообразования может быть обусловлена контактом крови с экстракорпоральной системой в ходе ГД. Этот эффект особенно характерен для диализаторов на основе купрофана и менее и выражен при использовании других мембран [123,124]. Тромбообразование менее выражено при использовании фракционного гепарина, чем нефракционного [34].…”

Section: нарушения коагуляцииunclassified

Epidemiologiya i patogeneticheskie faktory sindroma diabeticheskoy stopy u bol'nykh s terminal'noy stadiey khronicheskoy pochechnoy nedostatochnosti, nakhodyashchikhsya na dialize

Bublik¹,

Galstyan²

2007

Diabetes mellitus

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“…, cytokine production [3,4] , protein absorption [5] , platelet activation [1,6,7] , and oxidative stress [8][9][10] .…”

Section: Upar (Cd87) As a Biocompatibility Marker Of Dialysis Membranementioning

confidence: 99%

uPAR (CD87) as a Biocompatibility Marker of Dialysis Membrane

et al. 2006

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Background/Aims: Hemodialysis (HD) therapy may lead to functional changes in patient leukocytes. For example, the upregulation of inflammatory cytokines, such as IL-1β and TNFα, has been well characterized. However, these findings do not explain the entire response of leukocytes in HD. In this study, we carried out a comprehensive gene expression analysis in leukocytes treated with various dialysis membranes using DNA microarrays. The identified gene has the potential to be a new marker for testing dialysis membrane biocompatibility. Methods: Gene expression profiles were compared between a group of leukocytes treated with various dialysis membranes and an untreated group by using DNA microarray analysis. Expression was confirmed by quantitative RT-PCR. The expression of the gene product (leukocyte surface protein) was examined in 20 chronic HD patients by flow cytometry. Results: In addition to the inflammatory cytokines, the urokinase plasminogen activator receptor (uPAR or CD87) gene was induced in leukocytes treated with each dialysis membrane. The extent of induction depended on the membrane’s material composition. The expression of the uPAR (CD87) protein on leukocytes was markedly increased in patients undergoing dialysis therapy. The magnitude of uPAR (CD87) protein expression was correlated with clinical findings, i.e., the degree of leukopenia and the expression of adhesion molecules. Conclusions: The gene and protein expression of uPAR (CD87) depended on the dialysis membrane material and correlated closely with clinical findings. These results suggest that uPAR has the potential to serve as a marker not only for clinical use but also for the development of new dialysis membranes.

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In Vivo Evaluation of Platelet Activation by Different Cellulosic Membranes

Cited by 16 publications

References 16 publications

Platelet activation through interaction with hemodialysis membranes induces neutrophils to produce reactive oxygen species

Platelet activation through interaction with hemodialysis membranes induces neutrophils to produce reactive oxygen species

Epidemiologiya i patogeneticheskie faktory sindroma diabeticheskoy stopy u bol'nykh s terminal'noy stadiey khronicheskoy pochechnoy nedostatochnosti, nakhodyashchikhsya na dialize

uPAR (CD87) as a Biocompatibility Marker of Dialysis Membrane

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