In vivo evaluation of porous lithium-doped hydroxyapatite scaffolds for the treatment of bone defect

Luo, Yue; Li, Donghai; Zhao, Jianwu; Yang, Zhouyuan

doi:10.3233/bme-181018

Cited by 26 publications

(30 citation statements)

References 56 publications

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“…For osteogenesis studies, HA is used as implant coating, granules, or block bulk structures which act as non-resorbable materials in the short term, but still subjected to partial degradation and metabolization over time [149]. However, in addition to having poor mechanical properties, scaffolds of pure HA do not significantly promote vascularization and osteoinductivity [17]. Hence, additional doping is usually performed to augment osteoregeneration.…”

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

“…Due to improved fracture toughness, Mg- [27,204,205,206] and Zn-doped [207,208,209] HAs are most frequently tested for osteoregenerative properties; however, the incorporation of other ions was also tested. Lithium-doped HA scaffolds, for example, demonstrated improved mechanical properties and increased osteogenesis potential over pure HA [17]. The rationale of Li-doping is supported by several in vitro studies showing its role in osteogenic progeny growth and development, as well as in shifting mesenchymal stem cell fate toward osteogenic differentiation (as reviewed in References [1,210]).…”

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

“…The rationale of Li-doping is supported by several in vitro studies showing its role in osteogenic progeny growth and development, as well as in shifting mesenchymal stem cell fate toward osteogenic differentiation (as reviewed in References [1,210]). The main drawback of Li–HA is the lack of angiogenic stimulation [17,211], which can be overcome by including it in a composite [212]. Strontium doping was also favorable for osteogenesis when compared to pure HA in calvarial bone defect models [213], as well as in osteoporosis models [214,215,216].…”

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

“…When designed as resorbable biomaterials with various resorption kinetics (spanning from days to months), their ion dissolution products (Ca 2+ , (PO 4 ) 3− , Na + , Si4 + , Mg 2+ , and Sr 2+ ions) can usually be processed via normal metabolism [14] or can even be exploited to exert desired therapeutic effects, such as the promotion of angiogenesis or osteogenesis properties, and antimicrobial activity [11,15]. This new generation of CaP-based materials is envisaged to be employed in healthcare in various shapes and forms: bulk (especially for bone graft substitutes, e.g., porous scaffolds) [16,17,18], highly crystalline or nano-structured implant coatings [19], and dispersed nanoparticles (e.g., as antimicrobials or carriers in biological systems for drug delivery, transfection, gene silencing, or imaging) [20,21,22] or nano-objects (e.g., nano-rods, nano-wires, nano-tubes, and nano-needles) [23,24,25,26].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

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Recently, a large spectrum of biomaterials emerged, with emphasis on various pure, blended, or doped calcium phosphates (CaPs). Although basic cytocompatibility testing protocols are referred by International Organization for Standardization (ISO) 10993 (parts 1–22), rigorous in vitro testing using cutting-edge technologies should be carried out in order to fully understand the behavior of various biomaterials (whether in bulk or low-dimensional object form) and to better gauge their outcome when implanted. In this review, current molecular techniques are assessed for the in-depth characterization of angiogenic potential, osteogenic capability, and the modulation of oxidative stress and inflammation properties of CaPs and their cation- and/or anion-substituted derivatives. Using such techniques, mechanisms of action of these compounds can be deciphered, highlighting the signaling pathway activation, cross-talk, and modulation by microRNA expression, which in turn can safely pave the road toward a better filtering of the truly functional, application-ready innovative therapeutic bioceramic-based solutions.

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Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

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“…Recently, the preconditioning using lithium has been found bene cial in cell based therapy against disease models in brain, bone and heart. [17][18][19][20][21]Lithium could promote the proliferation of bone marrow derived mesenchymal stem cell (BMSC) via a glycogen synthase kinase (GSK) 3β-dependent β-catenin/Wnt pathway. [22] Besides, the preconditioning with Lithium has been found to reduce cell apoptosis by inducing autophagy.…”

Section: Introductionmentioning

confidence: 99%

The Preconditioning of Lithium promotes Mesenchymal Stem Cell Based Therapy for the Degenerated Intervertebral Disc via Upregulating Cellular ROS

Zeng

Xing

Tang

et al. 2020

Preprint

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BackgroundAdipose-derived stem cell (ADSC) is one of the most widely used candidate cell for intervertebral disc (IVD) degeneration. However, the poor survival and low differentiation efficacy in stressed host microenvironment limit the therapeutic effects of cell therapy. The preconditioning has been found effective to boost the proliferation and the functioning of stem cells in varying pathological condition. Lithium is a common anti-depression drug and has been proved effective to enhance stem cell functioning. In this study, the effects of preconditioning using LiCl on the cellular behavior of ADSC was investigated, and specially in a degenerative IVD-like condition.MethodThe cellular toxicity on ADSC was assessed by detecting lactate dehydrogenase (LDH) production after treatment with a varying concentration of lithium chloride (LiCl). The proliferative capacity of ADSC was determined by detecting Ki67 expression and the relative cell number of ADSC. Then, the preconditioned ADSC was challenged by a degenerative IVD-like condition. Meanwhile the cell viability and the nucleus pulpous (NP) cell differentiation efficacy of preconditioned ADSC were evaluated by detecting the major markers expression and extracellular matrix (ECM) deposit.ResultsA concentration range of 1-10 mmol/L of LiCl was applied in the following study, since a higher concentration of LiCl causes a major cell death (about 40%). The relative cell number was similar between preconditioned groups and the control group after preconditioning. The Ki67 expression was elevated after preconditioning. Consistently, the preconditioned ADSC showed stronger proliferation capacity. Besides, the preconditioned groups exhibit higher expression of NP markers than the control group after NP cell induction. Moreover, the preconditioning of LiCl reduced the cell death and promoted ECM deposits, when challenged with a degenerative IVD-like culture. Mechanically, the preconditioning of LiCl induced an increased cellular reactive oxidative species (ROS) level, which was found closely related to the enhanced cell survival and ECM deposits after preconditioning.ConclusionThese results suggest that the preconditioning with a medium level of LiCl boosts the cell proliferation and differentiation efficacy under a normal or hostile culture condition via upregulating cellular ROS level. It is a promising pre-treatment of ADSC to promote the cell functioning and the following regenerative capacity.

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Freeze casting of hydroxyapatite‐titania composites for bone substitutes

Yin,

Steyl,

Howard

et al. 2023

J Biomedical Materials Res

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Hydroxyapatite (HA) is commonly used as a bone substitute material, but it lacks mechanical strength when compared to native bone tissues. To improve the efficacy of HA as a bone substitute by improving the mechanical strength and cell growth attributes, porous composite scaffolds of HA and titania (HA‐TiO2) were fabricated through a freeze‐casting process. Three different compositions by weight percent, 25–75 HA‐TiO2, 50–50 HA‐TiO2, and 75–25 HA‐TiO2, were custom‐made for testing. After sintering at 1250°C, these composite scaffolds exhibited improved mechanical properties compared to porous HA scaffolds. Substrate mixing was observed, which helped reduce crystal size and introduced new phases such as β‐TCP and CaTiO3, which also led to improved mechanical properties. The composition of 50–50 HA‐TiO2 had the highest ultimate compressive strength of 3.12 ± 0.36 MPa and elastic modulus 63.29 ± 28.75 MPa. Human osteoblast cell proliferation assay also increased on all three different compositions when compared to porous HA at 14 days. These results highlight the potential of freeze casting composites for the fabrication of bone substitutes, which provide enhanced mechanical strength and biocompatibility while maintaining porosity.

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In vivo evaluation of porous lithium-doped hydroxyapatite scaffolds for the treatment of bone defect

Cited by 26 publications

References 56 publications

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

The Preconditioning of Lithium promotes Mesenchymal Stem Cell Based Therapy for the Degenerated Intervertebral Disc via Upregulating Cellular ROS

Freeze casting of hydroxyapatite‐titania composites for bone substitutes

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