In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): a new platelet concentrate

Tunalı, Mustafa; Özdemir, Hakan; Küçükodacı, Zafer; Akman, Serhan; Fıratlı, Erhan

doi:10.1016/j.bjoms.2012.08.003

Cited by 100 publications

(125 citation statements)

References 19 publications

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“…The results of the present study indicate that the PRF clot forms a mechanically strong fibrin matrix with a 3D architecture in which plenty of the platelets and leukocytes from the harvested blood are concentrated in the lower red region of the clot. This is concurrent with several previous reports (34,61). Platelets are activated during the prepared process, which leads to a substantial embedding of platelet growth factors into the fibrin matrix (62), including platelet-derived growth factor, insulin-like growth factors, transforming growth factor β, VEGF and a myriad of other substances which promote bone healing.…”

Section: Discussionsupporting

confidence: 93%

Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet-rich fibrin granules for bone regeneration in a rabbit calvarial critical-size defect model

Wang

Guo

et al. 2017

Experimental and Therapeutic Medicine

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The reconstruction of large bone defects remains a major clinical challenge, and tissue engineering is a promising technique for resolving this problem. Many attempts have been made to optimize bone tissue engineering protocols. The aim of the present study was to develop a process incorporating mesenchymal stem cell (MSC) sheets with nanoscale hydroxyapatite (nano-HA) and autologous platelet-rich fibrin (PRF) granules for enhanced bone formation within a critical-sized rabbit cranial defect. MSC sheets and PRF were prepared prior to in vivo experiments. The osteogenic differentiation ability of MSCs and the ultrastructure of PRF were also studied. A total of 15 New Zealand white rabbits were used in the current study and critical-size defects (CSDs) were surgically introduced in the cranium (diameter, 15 mm). The surgical defects were treated with MSC/PRF composites, MSC composites or left empty. Animals were euthanized at week 8 post-surgery. Iconography, histological and histomorphometric analysis were performed to assess de novo bone formation. The percentage of new bone in the MSC/PRF group (35.7±5.1%) was significantly higher than that in the MSC (18.3±3.2%; P<0.05) and empty defect groups (4.7±1.5%; P<0.05). The results of the present study suggest that combined application of an MSC sheet with nano-HA and granular PRF enhances bone regeneration in a rabbit calvarial CSD model, and provides a novel insight into bone tissue regeneration for large bone defects.

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Section: Discussionsupporting

confidence: 93%

Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet-rich fibrin granules for bone regeneration in a rabbit calvarial critical-size defect model

Wang

Guo

et al. 2017

Experimental and Therapeutic Medicine

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“…This difference may be due to a better hemocompatibility of titanium compared to glass, which could have potentially led to the formation of a more polymerized fibrin [26]. Due to this structure, we can hypothesize that T-PRF may last a bit longer in the tissue [20]. The SEM examination showed no difference between the two groups in platelet aggregation or the presence of leucocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Despite these findings and the successful results in clinical studies, we have modified the initial L-PRF method by changing the structure of the tubes and used a more biocompatible material, titanium [20]. This material was tried to eliminate the speculations about the potential negative effects of silica from dry glass or glass-coated plastic tubes.…”

Section: Introductionmentioning

confidence: 99%

A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin

Tunalı

Özdemir

Küçükodacı

et al. 2014

BioMed Research International

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We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF). The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun's leukocyte- and platelet-rich fibrin (L-PRF) method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF. Blood samples were collected from 10 healthy male volunteers. The blood samples were drawn using a syringe. Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube. Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF) was processed with a scanning electron microscope (SEM). The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis. The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples. This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product. The platelet activation by titanium seems to offer some high characteristics to T-PRF.

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“…Authors demonstrated that there was no influence of the type of tested tube on the architecture of this second generation PC. However Tunalı et al[33] in 2014, introduced a new product called T-PRF (Titanium-prepared PRF). The use of titanium tubes for collection and centrifugation instead of glass tubes was established on the hypothesis that titanium may be a more efficient platelet activator than silica, for preparing L-PRF.…”

Section: Recent Advancesmentioning

confidence: 99%

“…The use of titanium tubes for collection and centrifugation instead of glass tubes was established on the hypothesis that titanium may be a more efficient platelet activator than silica, for preparing L-PRF. Based on light, scanning electron and fluorescence microscopy analysis, Tunalı et al[33] concluded that T-PRF has immensely organized network along with a continuous integrity and even the fibrin network was thicker and also it covered larger area.…”

Section: Recent Advancesmentioning

confidence: 99%

Evolution, current status and advances in application of platelet concentrate in periodontics and implantology

Agrawal¹

2017

WJCC

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Platelet concentrates (PC) [platelet-rich plasma (PRP) and platelet-rich fibrin (PRF)] are frequently used for surgical procedures in medical and dental fields, particularly in oral and maxillofacial surgery, plastic surgery and sports medicine. The objective of all these technologies is to extract all the elements from a blood sample that could be used to improve healing and promote tissue regeneration. Although leukocyte rich and leukocyte poor PRP’s have their own place in literature, the importance of non-platelet components in a platelet concentrate remains a mystery. PC have come a long way since its first appearance in 1954 to the T-PRF, A-PRF and i-PRF introduced recently. These PC find varied applications successfully in periodontics and implant dentistry as well. However, the technique of preparation, standing time, transfer process, temperature of centrifuge, vibration, etc., are the various factors for the mixed results reported in the literature. Until the introduction of a proper classification of terminologies, the PC were known by different names in different countries and by different commercial companies which also created a lot of confusion. This review intends to clarify all these confusion by briefing the exact evolution of PC, their preparation techniques, recent advances and their various clinical and technical aspects and applications.

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In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): a new platelet concentrate

Cited by 100 publications

References 19 publications

Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet-rich fibrin granules for bone regeneration in a rabbit calvarial critical-size defect model

Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet-rich fibrin granules for bone regeneration in a rabbit calvarial critical-size defect model

A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin

Evolution, current status and advances in application of platelet concentrate in periodontics and implantology

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