In vivo experimental model of orthotopic dental pulp regeneration under the influence of photobiomodulation therapy

Moreira, Maria Stella; Diniz, Ivana Márcia Alves; Rodrigues, Maria Isabel; Carvalho, Rejane Andrade de; Carrer, Fernanda Campos de Almeida; Neves, Itamara Itagiba; Gavini, Giulio; Marques, Márcia Martins

doi:10.1016/j.jphotobiol.2016.11.022

Cited by 35 publications

(46 citation statements)

References 30 publications

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“…Most importantly, our results corroborate to the hypothesis that PBMT may be an adjunctive therapy able to improve the dental pulp tissue regeneration [50].…”

Section: Future Perspectives Of Pbmt and Ddmscssupporting

confidence: 87%

“…This study showed the feasibility of this method and suggests that it will become a useful tool for studies of novel therapeutic strategies for dental pulp regeneration under the influence of PBMT. Moreover, this protocol was able to generate a dental pulp-like tissue with vessels, nerves, an odontoblast-like cell layer, and perivascular stem cells [50].…”

Section: Future Perspectives Of Pbmt and Ddmscsmentioning

confidence: 99%

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Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature

et al. 2017

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This review aims to present and discuss the published data on the application of photobiomodulation therapy (PBMT) on cultured cells, including dentoalveolar-derived mesenchymal stem cells (ddMSCs). The knowledge of the effects of PBMT on ddMSCs is of importance for future application in tissue engineering approaches aiming to regenerate missing dental tissues, among others. The tissue engineering, which is based on the triad stem cells, growth factors, and scaffolds, presents shortcomings that could be circumvented by the effects of the PBMT. Thus, our research group has proposed the PBMT as a fourth element of tissue engineering. This proposition is based on the published data on the effects of PBMT on different cell types, including ddMSCs. Future perspectives on PBMT and ddMSCs will be presented showing translational studies of tissue regeneration induced by PBMT. The experience of our research group supported by other studies of the literature shows the beneficial effects of PBMT. Moreover, this review will show that PBMT on ddMSCs and tissue regeneration is still a huge field for future investigations not only in vitro but also in vivo.

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“…Most importantly, our results corroborate to the hypothesis that PBMT may be an adjunctive therapy able to improve the dental pulp tissue regeneration [50].…”

Section: Future Perspectives Of Pbmt and Ddmscssupporting

confidence: 87%

Section: Future Perspectives Of Pbmt and Ddmscsmentioning

confidence: 99%

Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature

et al. 2017

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“…4,24 Animal models have been employed to investigate the biological response of teeth with an open apex. 19,25 However, the present study was the first to evaluate, in vivo, the influence of PBM therapy for 30 days on root apexification and apexogenesis in a model of induced pulp necrosis. The main results show that PBM therapy favors apexification and apexogenesis in necrotic rat molars with an open apex, rejecting the null hypothesis.…”

Section: Discussionmentioning

confidence: 90%

Influence of photobiomodulation therapy on root development of rat molars with open apex and pulp necrosis

et al. 2019

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This study aimed to evaluate the role of photobiomodulation (PBM) in apexification and apexogenesis of necrotic rat molars with an open apex. Rat molars were exposed to the oral environment for 3 weeks. Canals were rinsed with 2.5% NaOCl and 17% EDTA, filled with antibiotic paste and sealed. After 7 days, canals were rinsed and divided into six groups (n=6): mineral trioxide aggregate (MTA); blood clot (BC); human dental pulp stem cells (hDPSC); MTA+PBM; BC+PBM; and hDPSC+PBM. In hDPSC groups, a 1% agarose gel scaffold was used. Two groups were not exposed: healthy tooth+PBM (n = 6), healthy tooth (n = 3); and one was exposed throughout the experiment: necrotic tooth (n = 3). In PBM groups, irradiation was performed with aluminum gallium indium phosphide (InGaAlP) diode laser for 30 days within 24-h intervals. After that, the specimens were processed for histological and immunohistochemical analyses. Necrotic tooth showed greater neutrophil infiltrate (p < 0.05). Necrotic tooth, healthy tooth, and healthy tooth+PBM groups showed absence of a thin layer of fibrous condensation in the periapical area. All the other groups stimulated the formation of a thicker layer of fibers (p < 0.05). All groups formed more mineralized tissue than necrotic tooth (p < 0.05). PBM associated with MTA, BC, or hDPSC formed more mineralized tissue (p < 0.05). MTA+PBM induced apexification (p < 0.05). Rabbit polyclonal anti-bone sialoprotein (BSP) antibody confirmed the histological findings of mineralized tissue formation, and hDPSC groups exhibited higher percentage of BSP-positive cells. It can be concluded that PBM improved apexification and favored apexogenesis in necrotic rat molars with an open apex.

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“…Recently, an investigation using an in vivo model showed that PBM therapy allowed the stem cells to be influenced by the growth factors released from the root canal walls, resulting in cell differentiation and tissue formation. 41 Also, molecular analyses indicated that red photobiomodulaton therapy in osteoblasts and hMSCs stem cells increased vinculin-rich clusters, osteogenic markers expression (Runx-2, alkaline phosphatase, osteopontin, Col I, OCN, DSPP, DMP1, and HSP27), and mineralized bone-like nodule structure deposition. [42][43][44] Adipogenic differentiation was previously investigated to characterize the stem cells or evaluate some materials.…”

Section: Discussionmentioning

confidence: 97%

Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model

et al. 2018

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Photobiomodulation therapy (PBM) has shown positive effects on stem cell differentiation in monolayer cell culture model, but little is known about its effect on three-dimensional (3-D) agarose gel culture. This study evaluated the PBM effect of human dental pulp stem cells (hDPSCs) differentiation and phosphatase alkaline activity (ALP) using an agarose 3-D model under different nutritional conditions. hDPSCs were characterized and seeded on a 0.3% agarose gel layer with different media (osteogenic, adipogenic, chondrogenic) and were assigned into four groups: control 10% fetal bovine serum (FBS), control 5% FBS, PBM 10% FBS, and PBM 5% FBS. Irradiation was performed with continuous-wave InGaAlP laser, 660 nm, 100 mW, 3,3 J / cm2, spot size 0.3 cm2, 10 s of exposure time, and 1 J of energy per point with 6-h interval between sessions. All groups were evaluated at 7 and 14 days. ALP assay was performed to analyze the deposition of mineralized tissue. At 7 days, PBM 5% FBS group presented better stimulation in osteogenic and adipogenic differentiation compared with control. After 14 days, hDPSCs cultured in 3-D exhibited osteogenic, adipogenic, and chondrogenic differentiation; furthermore, compared to control, PBM significantly stimulated all differentiation processes (p < 0.05). It can be concluded that hDPSCs cultured in 3-D agarose associated to PBM could be a promising tool for tissue engineering applications.

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In vivo experimental model of orthotopic dental pulp regeneration under the influence of photobiomodulation therapy

Cited by 35 publications

References 30 publications

Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature

Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature

Influence of photobiomodulation therapy on root development of rat molars with open apex and pulp necrosis

Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model

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