In vivo experimental study on bone regeneration in critical bone defects using an injectable biodegradable PLA/PGA copolymer

Rimondini, Lia; Nicoli-Aldini, N.; Fini, Milena; Guzzardella, Gaetano Antonio; Tschon, Matilde; Giardino, Roberto

doi:10.1016/j.tripleo.2004.05.010

Cited by 81 publications

(55 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[33][34][35][36][37][38] Recently, a noncritical-size defect smaller than 4-mm diameter and 6-mm depth, created in the distal femur of rabbits, was reported to be not totally filled with new bone after 1 year. 39 Although the untreated defects still maintained, as expected, the characteristics of nonhealed defects, a comparison of the healing rate of the current internal control defects (group 4: right side treated with FDBA þ PRP þ BMSC and left side left untreated) was made with that of external control defects, belonging to studies, where no proteins, cells, or growth factors were used, 34,38 in order to confirm that crossed influence did not occur. 9,29,40,41 No significant differences were actually highlighted (data not shown).…”

Section: Discussionmentioning

confidence: 99%

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Dallari

Fini

Stagni

et al. 2006

Journal Orthopaedic Research

156

View full text Add to dashboard Cite

The repair of confined trabecular bone defects in rabbits treated by autologous bone marrow stromal cells (BMSC), platelet-rich plasma (PRP), freeze-dried bone allografts (FDBA) alone and in combination (BMSC þ PRP; FDBA þ BMSC; FDBA þ PRP; FDBA þ PRP þ BMSC) was compared. A critical size defect was created in the distal part of the femurs of 48 adult rabbits. Histology and histomorphometry were used in the evaluation of healing at 2, 4, and 12 weeks after surgery. The healing rate (%) was calculated by measuring the residual bone defect area. Architecture of the newly formed bone was compared with that of bone at the same distal femur area of healthy rabbits. The defect healing rate was higher in PRP þ BMSC, FDBA þ PRP, FDBA þ BMSC, and FDBA þ PRP þ BMSC treatments, while lower values were achieved with PRP treatment at all experimental times. The highest bone-healing rate at 2 weeks was achieved with FDBA þ PRP þ BMSC treatment, which resulted significantly different from PRP (p < 0.05) and BMSC (p < 0.05) treatments. At 4 weeks, the bone-healing rate increased except for PRP treatment. Finally, the bone-healing rate of FDBA þ PRP, FDBA þ BMSC, and FDBA þ PRP þ BMSC was significantly higher than that of PRP at 12 weeks (p < 0.05). At 12 weeks, significant differences still existed between PRP, BMSC, and FDBA groups and normal bone (p < 0.05). These results showed that the combination of FDBA, BMSC and PRP permitted an acceleration in bone healing and bone remodeling processes. ß

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Dallari

Fini

Stagni

et al. 2006

Journal Orthopaedic Research

156

View full text Add to dashboard Cite

show abstract

“…However, this definition is not often applied to defects within animal models, and the important parameters to report are defect size and location. Efficacy of any tissue-engineered constructs within these criticalsized defects is dependent on a number of variables detailed in Table 1 (Lindsey et al, 2006;Reichert et al, 2009;Rimondini et al, 2005). Furthermore, when choosing a Table 1.…”

Section: Animal Model Selectionmentioning

confidence: 99%

Tissue engineered bone using select growth factors: A comprehensive review of animal studies and clinical translation studies in man

Gothard¹,

Smith²,

Kanczler³

et al. 2014

eCM

162

125

View full text Add to dashboard Cite

“…These biomaterials need to be easy to manipulate, be absorbable and have a physical structure capable of maintaining a space for bone formation without generating an appreciable immune response. Despite major advances in science and technology, there is not a substance that meets the majority of these requirements 13,14 .…”

Section: Discussionmentioning

confidence: 99%

The effect of simvastatin on the regeneration of surgical cavities in the femurs of rabbits

Rosselli

Martins

et al. 2014

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE:To evaluate the effect of a local application of simvastatin gel in repairing bone defects in the femurs of rabbits. METHODS:Two standard surgical cavities were created in the femoral epiphysis of 18 rabbits. In the simvastatin group (SG), the cavities were filled with a collagen sponge soaked in 0.5 ml of a simvastatin (1 mg) gel, and the cavities were covered with a biological membrane. The bone cavities in the second group (control group) were filled with a blood clot and covered with a biological membrane.On the 7 th, 21st and 42 nd days, six animals in each group were euthanized, and the femurs were subject to histological evaluation (vascularity, fibrosis, reactive bone formation, osteoblasts, and osteoclasts) and immunohistochemical (anti-VEGF and anti-osteocalcin) analysis.The results were analyzed using a Wilcoxon test (p<0.05). RESULTS:There were significant differences between the two groups: the SG had greater scores in comparison with the CG in terms of the degree of vascularity on the 7t h and the 21st days, fibrosis on the 21 st day, bone formation reaction on the 21 st and the 42 nd days and the number of osteoblasts and osteoclasts on the 42 nd day. The immunohistochemical expression was also greater for osteocalcin and VEGF on the 7 th, 21st and 42 nd days. CONCLUSION:Surgical defects created in rabbit femurs were treated locally with simvastatin gel to stimulate bone repair, which promoted an ameliorative effect in the morphological and immunohistochemical markers of bone regeneration.

show abstract

In vivo experimental study on bone regeneration in critical bone defects using an injectable biodegradable PLA/PGA copolymer

Cited by 81 publications

References 37 publications

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Tissue engineered bone using select growth factors: A comprehensive review of animal studies and clinical translation studies in man

The effect of simvastatin on the regeneration of surgical cavities in the femurs of rabbits

Contact Info

Product

Resources

About