In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae)

Zakri, Adel M.; Ziegler, Angelika; Commandeur, Ulrich; Fischer, Rainer; Torrance, L.

doi:10.1007/s00705-012-1310-2

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…In an approach known as "immunomodulation", transgenic plants express antibodies against viral proteins. Single-chain antibodies generated against the geminiviral coat protein were shown to bind and provide resistance in vivo (Zakri et al, 2012). Similarly, anti-Rep antibody expression can provide resistance, but the response varies between lines due to variable transgene expression (Safarnejad et al, 2009).…”

Section: Interfering With Viral Proteinsmentioning

confidence: 99%

Geminivirus Resistance: A Minireview

Beam

Ascencio-Ibáñez

2020

Front. Plant Sci.

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A continuing challenge to crop production worldwide is the spectrum of diseases caused by geminiviruses, a large family of small circular single-stranded DNA viruses. These viruses are quite diverse, some containing mono-or bi-partite genomes, and infecting a multitude of monocot and dicot plants. There are currently many efforts directed at controlling these diseases. While some of the methods include controlling the insect vector using pesticides or genetic insect resistance (Rodrí guez-Ló pez et al., 2011), this review will focus on the generation of plants that are resistant to geminiviruses themselves. Genetic resistance was traditionally found by surveying the wild relatives of modern crops for resistance loci; this method is still widely used and successful. However, the quick rate of virus evolution demands a rapid turnover of resistance genes. With better information about virus-host interactions, scientists are now able to target early stages of geminivirus infection in the host, preventing symptom development and viral DNA accumulation.

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Section: Interfering With Viral Proteinsmentioning

confidence: 99%

Geminivirus Resistance: A Minireview

Beam

Ascencio-Ibáñez

2020

Front. Plant Sci.

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Antibody-Mediated Pathogen Resistance in Plants

Peschen

Schillberg

Fischer

2016

Methods in Molecular Biology

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The methods described in this chapter were developed in order to produce transgenic plants expressing pathogen-specific single-chain variable fragment (scFv) antibodies fused to antifungal peptides (AFPs), conferring resistance against fungal pathogens. We describe the selection from a phage display library of avian scFv antibodies that recognize cell surface proteins on fungi from the genus Fusarium, and the construction of scFv-AFP fusion protein constructs followed by their transient expression in tobacco (Nicotiana spp.) plants and stable expression in Arabidopsis thaliana plants. Using these techniques, the antibody fusion with the most promising in vitro activity can be used to generate transgenic plants that are resistant to pathogens such as Fusarium oxysporum f. sp. matthiolae.

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Natural and engineered host resistance for geminivirus management

Yogindran

Chakraborty

2022

Geminivirus : Detection, Diagnosis and Management

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In vivo expression and binding activity of scFv-RWAV, which recognizes the coat protein of tomato leaf curl New Delhi virus (family Geminiviridae)

Cited by 3 publications

References 45 publications

Geminivirus Resistance: A Minireview

Geminivirus Resistance: A Minireview

Antibody-Mediated Pathogen Resistance in Plants

Natural and engineered host resistance for geminivirus management

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