In vivo fertilization after initiation of sperm motility in the hamster epididymis

Kann, Marie-Louise; Raynaud, Françoise

doi:10.1051/rnd:19820402

Cited by 8 publications

(4 citation statements)

References 17 publications

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“…A report published earlier showed that when the uteri of superovulated female hamsters were inseminated with caput or cauda spermatozoa, caput spermatozoa failed to fertilize eggs, whereas cauda spermatozoa achieved 88% success [20]. Clinical data also suggest that pregnancy outcomes after cauda epididymal anastomoses are superior to those after caput anastomoses [22]. Given this information, we were not surprised to find that, in our group, the majority (95%) of patency cases undergoing LIVE at the cauda had PMS in their ejaculate, and the percentage of PMS was relatively high.…”

Section: Discussionmentioning

confidence: 99%

Clinical observation of loupe-assisted intussusception vasoepididymostomy in the treatment of obstructive azoospermia (analysis of 49 case reports)

Zhang¹,

Wei²,

Xu³

et al. 2009

Asian J Androl

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To evaluate the clinical outcomes of loupe-assisted intussusception vasoepididymostomy (VE) in the treatment of epididymal obstructive azoospermia (EOA), we retrospectively analyzed data from 49 patients with EOA who underwent two-suture longitudinal intussusception vasoepididymostomy (LIVE) between 2000 and 2007. The data included the surgical method, postoperative motile sperm count per ejaculation, percentage of progressive motile sperm and patency and pregnancy outcomes. There were a total of 49 men undergoing scrotal exploration, and epididymal obstruction was found in all cases. Bilateral or unilateral anastomoses were performed in 40 and 6 men, respectively. The postoperative courses of 42 patients were followed up for more than 6 months, and the courses of 38 patients were followed up for more than 1 year. The overall patency and pregnancy rates were 71.4% and 26.3%, respectively. Moreover, progressive motile sperm was more frequently present in those patients who had undergone anastomosis at cauda than at corpus or caput. Pregnancy was achieved only in those patients who had undergone anastomosis at least on one side of the cauda epididymis. We think that the loupe-assisted method, with a lower overall cost and a simplified surgical procedure, can achieve satisfactory patency outcomes and pregnancy results. Data from this paper also suggest that paternity outcomes occur more frequently after anastomoses at cauda than at corpus or caput.

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Section: Discussionmentioning

confidence: 99%

Clinical observation of loupe-assisted intussusception vasoepididymostomy in the treatment of obstructive azoospermia (analysis of 49 case reports)

Zhang¹,

Wei²,

Xu³

et al. 2009

Asian J Androl

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show abstract

“…This may indicate changes in the outer dense fibres but the factors controhng this are not known. Circular motion, while demonstrated to be incompatible with transport through the uterotubal junction in rats (Gaddum-Rosse, 1981) and hamsters (Kann &Raynaud, 1982), and presumably so in the mouse (Pavlok, 1974), does permit fusion with the vitellus in sub-zonal insemination in mice (Lacham & Trounson, 1991). A change in flagellar stiffness, possibly related to oxidation of disulphide bridges in the outer dense fibres, may bring about the forward progression seen in spermatozoa on maturation, as observed in the ham- istributions of values of three derived ster (Cornwall ef al., 1986;Yeung et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Development of sperm motility patterns in the murine epididymis

Soler

Yeung²,

Cooper³

1994

Int J of Andrology

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The maturation of sperm motility in the epididymis of the mouse was assessed using a computer-assisted sperm analysis system. Spermatozoa were immotile in the most proximal regions of the epididymis but developed motility rapidly in the proximal caput epididymis; the percentage motility remained high thereafter. In the caput, flagellar beat was erratic with little progression, but in the proximal corpus region circular movement patterns were reflected in reduced linearity (LIN) and straightness (STR) of the sperm tracks, although velocities were little changed and wobble (WOB) increased. In the mid-corpus region, however, all velocities, LIN, STR and WOB, increased markedly. In more distal regions there was little change in these parameters. Distribution curves of the kinematic parameters of spermatozoa obtained from each region indicated that the most heterogeneous population was that from the mid-corpus epididymis; the most homogeneous was that from the mid-cauda region. Individual sperm tracks revealed slowly progressing spermatozoa in the distal caput, transforming to motion in small circles, interrupted by more linear progression. More distally, linear progression was interrupted by looping movements and a generally progressive path was observed thereafter, with less deviation from the average path as the spermatozoa matured. Spermatozoa displaying motion compatible with passing the uterotubal junction were first found in the proximal corpus epididymis, in agreement with earlier in-vivo fertilization studies on where fertilizing capacity is achieved with epididymal spermatozoa.

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“…The anticipated rapid postejaculatory changes are in marked contrast to responses of somatic cells, where the need for RVD rarely arises (O'Neill, 1999). Kann and Raynaud (1982) demonstrated that hamster caput sperm, stimulated to motility by caffeine, failed to fertilize eggs when inseminated into the uterus and exhibited angulated flagella (reflecting the swollen state). However, 22% of eggs were fertilized when sperm were pretreated with forward motility protein to prevent flagellar angulation.…”

Section: Importance Of Volume Regulationmentioning

confidence: 97%

Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet

Cooper¹,

Yeung²

2003

Microscopy Res & Technique

171

134

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In certain cases of infertility in domestic species, and in the homozygous c-ros tyrosine kinase knockout mouse, males are infertile as a result of unopposed sperm swelling. This induces flagellar angulation, preventing their normal migration in the female tract. Angulation always occurs at the site of the cytoplasmic droplet located in mature sperm at the annulus (midpiece - principal piece junction). This article reviews the literature on the fate of the sperm's cytoplasmic droplet and suggests a role for it in volume regulation. A hypothesis is presented that during epididymal transit sperm acquire osmolytes accumulated by the epididymal epithelium. This is possibly driven by a mechanism of regulatory volume increase invoked by the hypertonicity of epididymal luminal fluid. These osmolytes are subsequently used for regulatory volume decrease after ejaculation into the relatively hypotonic female tract. Study of the mechanisms of sperm volume regulation may highlight new contraceptive leads.

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In vivo fertilization after initiation of sperm motility in the hamster epididymis

Cited by 8 publications

References 17 publications

Clinical observation of loupe-assisted intussusception vasoepididymostomy in the treatment of obstructive azoospermia (analysis of 49 case reports)

Clinical observation of loupe-assisted intussusception vasoepididymostomy in the treatment of obstructive azoospermia (analysis of 49 case reports)

Development of sperm motility patterns in the murine epididymis

Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet

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