In Vivo Function and Evolution of the Eutherian-Specific Pluripotency Marker UTF1

Nishimoto, Masazumi; Katano, Miyuki; Yamagishi, Toshiyuki; Hishida, Tomoaki; Kamon, Masayoshi; Suzuki, Ayumu; Hirasaki, Masataka; Nabeshima, Yoko; Nabeshima, Yo‐ichi; Katsura, Yoshiteru; Satta, Yoko; Deakin, Janine E.; Graves, Jennifer A. Marshall; Kuroki, Yoshio; Ono, Ryuichi; Ishino, Fumitoshi; Ema, Makoto; Takahashi, Satoru; Kato, Hidemasa; Okuda, Akihiko

doi:10.1371/journal.pone.0068119

Cited by 18 publications

(24 citation statements)

References 47 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of Sall4 in SSCs has been a challenge to decipher because of the early lethality of Sall4 -null mice [122]. In contrast, Utf1 -null mice have surprisingly mild defects, raising the possibility of redundancy with other transcription factors or context-specific effects [124]. Another candidate to have a role in SSCs is the transcription factor RHOX10, which is encoded by a member of a large X-linked homeobox gene cluster selectively expressed in the reproductive tract [125].…”

Section: Perspectivementioning

confidence: 99%

Transcriptional control of spermatogonial maintenance and differentiation

Song

Wilkinson

2014

Seminars in Cell & Developmental Biology

115

View full text Add to dashboard Cite

Spermatogenesis is a multistep process that generates millions of spermatozoa per day in mammals. A key to this process is the spermatogonial stem cell (SSC), which has the dual property of continually renewing and undergoing differentiation into a spermatogonial progenitor that expands and further differentiates. In this review, we will focus on how these proliferative and early differentiation steps in mammalian male germ cells are controlled by transcription factors. Most of the transcription factors that have so far been identified as promoting SSC self-renewal (BCL6B, BRACHYURY, ETV5, ID4, LHX1, and POU3F1) are upregulated by glial cell line-derived neurotrophic factor (GDNF). Since GDNF is crucial for promoting SSC self-renewal, this suggests that these transcription factors are responsible for coordinating the action of GDNF in SSCs. Other transcription factors that promote SSC self-renewal are expressed independently of GDNF (FOXO1, PLZF, POU5F1, and TAF4B) and thus may act in non-GDNF pathways to promote SSC cell growth or survival. Several transcription factors have been identified that promote spermatogonial differentiation (DMRT1, NGN3, SOHLH1, SOHLH2, SOX3, and STAT3); some of these may influence the decision of an SSC to commit to differentiate while others may promote later spermatogonial differentiation steps. Many of these transcription factors regulate each other and act on common targets, suggesting they integrate to form complex transcriptional networks in self-renewing and differentiating spermatogonia.

show abstract

Section: Perspectivementioning

confidence: 99%

Transcriptional control of spermatogonial maintenance and differentiation

Song

Wilkinson

2014

Seminars in Cell & Developmental Biology

115

View full text Add to dashboard Cite

show abstract

“…A subsequent Utf1 ko ( Utf1 (−/−) ) mouse study, however, revealed that Utf1 is not critical for pluripotency, because viable and fertile Utf1 (−/−) mice can be obtained 14 . A noticeable Utf1 (−/−) phenotype reported in this study was developmental delay during embryogenesis that often caused smaller pups and neonatal death, which was attributed to reduced placental growth.…”

Section: Introductionmentioning

confidence: 99%

“…A noticeable Utf1 (−/−) phenotype reported in this study was developmental delay during embryogenesis that often caused smaller pups and neonatal death, which was attributed to reduced placental growth. However, the delay can be resolved during neonatal growth, thus resulting in phenotypically normal adult Utf1 (−/−) mice 14 . The in vivo relevance of Utf1-controlled maintenance of pluripotency, as proposed for ESCs in vitro , therefore remains an unresolved issue 13 , 15 .…”

Section: Introductionmentioning

confidence: 99%

Utf1 contributes to intergenerational epigenetic inheritance of pluripotency

Bao

Morshedi

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Undifferentiated embryonic cell transcription factor 1 (Utf1) is expressed in pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs). Utf1 expression is directly controlled by pluripotency factors Oct4 and Sox2, which form a ternary complex with the Utf1 enhancer. The Utf1 protein plays a role in chromatin organization and epigenetic control of bivalent gene expression in ESCs in vitro, where it promotes effective cell differentiation during exit from pluripotency. The function of Utf1 in PGCs in vivo, however, is not known. Here, we report that proper development of Utf1 null embryos almost entirely depends on the presence of functional Utf1 alleles in the parental germline. This indicates that Utf1’s proposed epigenetic role in ESC pluripotency in vitro may be linked to intergenerational epigenetic inheritance in vivo. One component - or at least facilitator - of the relevant epigenetic mark appears to be Utf1 itself, since Utf1-driven tomato reporter and Utf1 are detected in mature germ cells. We also provide initial evidence for a reduced adult testis size in Utf1 null mice. Our findings thus point at unexpected functional links between the core ESC pluripotency factor network and epigenetic inheritance of pluripotency.

show abstract

“…Notably, Esrrb null embryos survive until 10.5 days post coitum (dpc) and die due to placental abnormalities [73]. Similarly, while Dppa4, Dppa5 and Utf1 are specifically expressed in pluripotent cells in vivo and in culture, the impact of their absence is only observed during late embryonic stages, though redundancy among these genes has not yet been investigated [74][75][76].…”

Section: Regulatory Network For Pluripotency and Lineage Commitmentmentioning

confidence: 99%

From blastocyst to gastrula: gene regulatory networks of embryonic stem cells and early mouse embryogenesis

Parfitt

Shen

2014

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

To date, many regulatory genes and signalling events coordinating mammalian development from blastocyst to gastrulation stages have been identified by mutational analyses and reverse-genetic approaches, typically on a geneby-gene basis. More recent studies have applied bioinformatic approaches to generate regulatory network models of gene interactions on a genome-wide scale. Such models have provided insights into the gene networks regulating pluripotency in embryonic and epiblast stem cells, as well as cell-lineage determination in vivo. Here, we review how regulatory networks constructed for different stem cell types relate to corresponding networks in vivo and provide insights into understanding the molecular regulation of the blastocyst-gastrula transition.

show abstract

In Vivo Function and Evolution of the Eutherian-Specific Pluripotency Marker UTF1

Cited by 18 publications

References 47 publications

Transcriptional control of spermatogonial maintenance and differentiation

Transcriptional control of spermatogonial maintenance and differentiation

Utf1 contributes to intergenerational epigenetic inheritance of pluripotency

From blastocyst to gastrula: gene regulatory networks of embryonic stem cells and early mouse embryogenesis

Contact Info

Product

Resources

About