In Vivo Fusion of Circulating Fluorescent Cells with Dystrophin-Deficient Myofibers Results in Extensive Sarcoplasmic Fluorescence Expression but Limited Dystrophin Sarcolemmal Expression

Chrétien, Fabrice; Dreyfus, Patrick A.; Christov, Christo; Caramelle, Philippe; Lagrange, Jean‐Léon; Chazaud, Bénédicte; Gherardi, Romain K.

doi:10.1016/s0002-9440(10)62484-4

Cited by 37 publications

(34 citation statements)

References 28 publications

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“…Six months after transplantation, TA muscle transections immunostained for basal lamina markers (laminin or collagen IV) showed a total number of 94 GFPϩ cells, 38 of which were sublaminal ( Figure 1D). Coimmunostaining showed nuclear Pax7 expression in a proportion of GFP-positive cells (n ϭ 7), as previously reported (Dreyfus et al, 2004;Chretien et al, 2005). Vascular proximity of these cells became obvious when collagen IV immunostaining was done as a supplementary reaction and merged with initial images of Pax7ϩ and GFPϩ cells (Figure 1, E-G).…”

supporting

confidence: 83%

“…Although sublaminal SCs are derived mainly from somites (Gros et al, 2005), a small numbers of SCs may be generated from bone marrow grafts (LaBarge and Blau, 2002;Dreyfus et al, 2004). These ectopic SCs express canonic SC markers (Dreyfus et al, 2004;Chretien et al, 2005) but exhibit very limited myogenic potential once reisolated from muscle (Sherwood et al, 2004). Thus, they might represent foreign stem cells squatting in SC niches.…”

Section: Juxtavascular Niches Can Incorporate Bone Marrow-derived Cellsmentioning

confidence: 99%

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Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners

Christov

Chrétien

Abou-Khalil

et al. 2007

MBoC

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577

601

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Genetically engineered mice (Myf5 nLacZ/؉ , Myf5GFP-P/؉ ) allowing direct muscle satellite cell (SC) visualization indicate that, in addition to being located beneath myofiber basal laminae, SCs are strikingly close to capillaries. After GFP ؉ bone marrow transplantation, blood-borne cells occupying SC niches previously depleted by irradiation were similarly detected near vessels, thereby corroborating the anatomical stability of juxtavascular SC niches. Bromodeoxyuridine pulse-chase experiments also localize quiescent and less quiescent SCs near vessels. SCs, and to a lesser extent myonuclei, were nonrandomly associated with capillaries in humans. Significantly, they were correlated with capillarization of myofibers, regardless to their type, in normal muscle. They also varied in paradigmatic physiological and pathological situations associated with variations of capillary density, including amyopathic dermatomyositis, a unique condition in which muscle capillary loss occurs without myofiber damage, and in athletes in whom capillaries increase in number. Endothelial cell (EC) cultures specifically enhanced SC growth, through IGF-1, HGF, bFGF, PDGF-BB, and VEGF, and, accordingly, cycling SCs remained mainly juxtavascular. Conversely, differentiating myogenic cells were both proangiogenic in vitro and spatiotemporally associated with neoangiogenesis in muscular dystrophy. Thus, SCs are largely juxtavascular and reciprocally interact with ECs during differentiation to support angio-myogenesis. INTRODUCTIONMuscle tissue repair is a complex biological process that crucially involves activation of stem cells. Skeletal muscle contains two different stem cell types: 1) myogenic stem cells, so-called satellite cells (SCs), that reside beneath the basal lamina of muscle fibers (Mauro, 1961) and express both NCAM/CD56 and early myogenic cell markers such as M-cadherin, Pax7, and Myf5 (Beauchamp et al., 2000;Seale et al., 2000;Hawke and Garry, 2001; Bischoff and FranziniArmstrong, 2004) and 2) interstitial multipotent stem cells, which are extralaminal, exhibit fibroblastic morphology and do not express myogenic markers (Asakura et al., 2001;Tamaki et al., 2002). SCs are primarily quiescent in skeletal muscle, can self-renew (Collins et al., 2005) and upon activation, proliferate and further differentiate to become fusioncompetent myoblasts and ensure muscle regeneration (Hawke and Garry, 2001;Bischoff and Franzini-Armstrong, 2004). Interstitial "muscle-derived" stem cells give rise to several lineages after transplantation and in this setting, contribute to synchronized reconstitution of blood vessels (pericytes, smooth muscle cells [SMCs], and endothelial cells), peripheral nerve (Schwann cells), and muscle cells (myofibers and SCs; Tamaki et al., 2005). However, participation of multipotent interstitial stem cells in physiological muscle repair appears to be limited. Instead, it is widely accepted that sublaminal SCs represent the pre-eminent muscle stem cell type used for muscle growth, repair and regeneration (Dhawan...

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confidence: 83%

Section: Juxtavascular Niches Can Incorporate Bone Marrow-derived Cellsmentioning

confidence: 99%

Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners

Christov

Chrétien

Abou-Khalil

et al. 2007

MBoC

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577

601

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“…The strategy is to deliver the protein missing from DMD muscle through the fusion of dystrophin-expressing myogenic cells with dystrophic myofibres. There is growing success in testing this modality of treatment (Chretien et al, 2005;Cossu and Mavilio, 2000;Cousins et al, 2004;Gussoni et al, 1999;Huard et al, 1994;Ikezawa et al, 2003;Jankowski and Huard, 2004;Li et al, 2005b;Liu et al, 2005;Partridge et al, 1989;Payne et al, 2005;Qu-Petersen et al, 2002).…”

Section: (2) Satellite Cells As a Conveyance In Skeletal Muscle Plastmentioning

confidence: 99%

The satellite cell as a companion in skeletal muscle plasticity:currency, conveyance, clue, connector and colander

Anderson

2006

Journal of Experimental Biology

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THE JOURNAL OF EXPERIMENTAL BIOLOGY 2277 Satellite cells in muscle plasticity formed cells fusing to form myoblasts and finally multinucleated muscle fibres.'That conference was a landmark in studies of the satellite cell and muscle regeneration, and was attended by many of the notable and pioneering contributors to the field of muscle development and muscle regeneration. Muir summarized the debate on a working definition of the satellite cell, as follows (Muir, 1970):'The satellite cell of striated muscle can be defined as a mononucleated cell, whose cytoplasm does not contain myofilaments, and which is enclosed by or lies within the basement membrane component of the sarcolemma of the striated muscle fibre. This definition does not exclude cells which are partially or completely separated from the muscle fibre plasma membrane by extensions of the basement membrane, providing that the basement membrane forms a complete investment of their outer surfaces. ' Although there were presentations detailing the observed uptake of 3 H-thymidine into nuclei of satellite cells (Hay, 1970), the role of satellite cells as precursors was not fully established in 1969. In fact there was still very strong debate that a muscle fibre could fragment into blastema cells that led to new formation of muscle. Even at this time, there was more than one paper on the appearance of osteogenic and chondrogenic tissues from minces of muscle replaced under the skin, and a report of muscle fibre nuclei contributed by a labelled connective tissue grafted into a salamander limb during regeneration (Steen, 1970).It wasn't until two seminal reports from the laboratory of Dr Charles Leblond at McGill University (Moss and Leblond, 1970;Moss and Leblond, 1971) that skeletal muscle satellite cells were convincingly identified as the source of precursor cells that proliferate and fuse to form new skeletal muscle fibres. Time-course studies of labelled nuclei in growing muscle showed that satellite cells were the only muscle cells that had incorporated 3 H-thymidine. The report followed work on colchicine-treated rats (MacConnachie et al., 1964) that showed mitotic figures only in the peripheral 'satellite' position on muscle fibres. The idea that muscle fibre nuclei give rise to the increasing number of myonuclei during the growth was convincingly ruled out (Enesco and Puddy, 1964). The notion that satellite cells contribute these domains to a growing or regenerating fibre, one at a time, is daunting in light of the expenditure of proliferative energy and fusion events. However, it speaks strongly to one of the major roles of satellite cells as a currency of muscle (see below). Satellite cells were also observed on intrafusal fibres (Katz, 1961). Two types of satellite cells were identified on these socalled muscle spindle fibres and were distinguished from those on extrafusal fibres (Maynard and Cooper, 1973), although at least one would now be called a myoblast.Although typically quiescent in normal adult muscle, satellite cells are generally con...

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“…could be more easily translated to therapy in patients. The first BMT in a dystrophic animal was performed in the mdx mouse, a model for DMD [28,29]. The authors found that some transplanted cells were able to engraft in the skeletal muscle, but not to express dystrophin.…”

mentioning

confidence: 99%

Bone Marrow Transplantation in Dysferlin-Deficient Mice Results in a Mild Functional Improvement

Flix

Suárez‐Calvet²,

Díaz‐Manera³

et al. 2013

Stem Cells and Development

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Dysferlinopathies are caused by mutations in the DYSF gene. Dysferlin is a protein mainly expressed in the skeletal muscle and monocytes. Cell therapy constitutes a promising tool for the treatment of muscular dystrophies. The aim of our study was to evaluate the effect of bone marrow transplantation (BMT) using the A/J Dysf prmd mouse model of dysferlinopathy. For that purpose, we studied dysferlin expression by western blot and/or immunohistochemistry in transplanted mice and controls. Computerized analyses of locomotion and electrophysiological techniques were also performed to test the functional improvement. We observed dysferlin expression in splenocytes, but not in the skeletal muscle of the transplanted mice. However, the locomotion test, electromyography studies, and muscle histology showed an improvement in all transplanted mice that was more significant in the animals transplanted with dysferlin + / + cells. In conclusion, although BMT restores dysferlin expression in monocytes, but not in skeletal muscle, muscle function was partially recovered. We propose that the slight improvement observed in the functional studies could be related with factors, such as the hepatocyte growth factor, released after BMT that prevented muscle degeneration.

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In Vivo Fusion of Circulating Fluorescent Cells with Dystrophin-Deficient Myofibers Results in Extensive Sarcoplasmic Fluorescence Expression but Limited Dystrophin Sarcolemmal Expression

Cited by 37 publications

References 28 publications

Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners

Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners

The satellite cell as a companion in skeletal muscle plasticity:currency, conveyance, clue, connector and colander

Bone Marrow Transplantation in Dysferlin-Deficient Mice Results in a Mild Functional Improvement

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