In Vivo Genotoxicity Testing Of Vitamin C And Naproxen Sodium Using Plant Bioassay

Cenanovic, Merisa; Durakovic, Camil

doi:10.21533/scjournal.v4i2.100

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…Our findings regarding mitotic index and chromosomal abnormalities are in agreement with the study reported in Allium cepa by Asita et al, (2017). However, Cenanovic and Durakovic (2016) reported that ascorbic acid treatment at different concentrations (250, 500 and1000 μg/ml) increased the mitotic index in Allium cepa root meristems. It is thought that this difference may have occurred depending on the plant species studied and/or the dose and application time of the ascorbic acid used.…”

Section: Resultssupporting

confidence: 92%

L-Ascorbic acid modulates the cytotoxic and genotoxic effects of salinity in barley meristem cells by regulating mitotic activity and chromosomal aberrations

2023

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The objective of the present study was to with all details explain of the efficiency of L-ascorbic acid (L-AsA) also known as vitamin C on cytotoxicity and genotoxicity induced by salt stress in the barley apical meristems. As a result of the statistical analysis salt stress caused a significant (P ≤ 0.05) decrease in mitotic index of barley seeds depending on concentration increase, while the frequency of chromosomal aberration (CA) increased. In addition, it was determined that mitotic index value was decreased by 46% with 1 μM L-AsA supplementation as compared to control and chromosomal abnormalities were increased by 8.96% as well as. However, in the case of simultaneously application of 1 μM L-AsA and different salt concentrations, the high salt concentrations exhibited an excellent success according to low salt concentrations in alleviating the mitodepressive effect of salt stress. Moreover, the frequency of chromosomal aberrations in the root meristem cells of those seeds with 1 μM L-AsA supplementation germinated at different salt concentrations was substantially reduced compared to own control group (alone 1 μM L-AsA pretreatment). The 1 μM L-AsA pretreatment at the highest salt concentration (at 0.40 M) was showed an excellent success by reducing the frequency of the chromosomal aberrations by approximately 90 %. Different salt concentrations and/or 1 μM L-AsA supplementation caused micronuclei and granulation as well as various chromosomal aberrations in prophase, metaphase, anaphase and telophase.

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Section: Resultssupporting

confidence: 92%

L-Ascorbic acid modulates the cytotoxic and genotoxic effects of salinity in barley meristem cells by regulating mitotic activity and chromosomal aberrations

2023

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“…Genotoxic studies on NP are rather scarce, and there is very little information on the potential genotoxic effects of NP despite its wide range of applications, which is mentioned by another author as well [12]. Nevertheless, it has been reported that NP can alter the biochemical biomarkers and genetic materials [13].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of naproxen-induced oxidative stress, hepatotoxicity and in-vivo genotoxicity in male Wistar rats

Ahmad

Fatima

Hossain

et al. 2018

Journal of Pharmaceutical Analysis

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Naproxen (NP), a nonsteroidal anti-inflammatory drug (NSAID), is used for the treatment of common pain, inflammation and tissue damage. Genotoxicity testing of NP is of prime importance as it represents the largest group of drugs to which humans are exposed. Not many genotoxic studies are reported on NP; therefore, the present study investigated the detailed genotoxic and oxidative stress properties of NP. Male Wistar rats were administered NP orally at the doses of 38.91 and 65.78 mg/kg body weight for 14 days. Reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation (LPO) activities/levels were measured in the liver, kidney and brain tissues. The aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) activities, and total bilirubin (TBIL) levels were measured in the liver tissues. Micronucleus frequency (micronucleus test MNT) and DNA damage (comet assay) were performed in the bone marrow cells and leukocytes, respectively. The results showed that NP treatment decreased the GSH levels and increased the SOD, CAT, LPO, ALT, AST, ALP and TBIL activities/levels compared to the control (p < 0.05). Results of MNT showed an increased micronucleus induction and comet assay showed a significant increase in DNA damage in the NP treated animals (p < 0.05). Treatment of NP resulted in the biochemical imbalance and induced oxidative stress that deteriorated the integrity of the cells, which caused significant damage to the genetic material and affected liver function in male Wistar rats. Therefore, NP is a potential genotoxic agent that induces genotoxicity and oxidative stress.

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“…In the present study, none of the concentrations of the hyraceum extract tested significantly increased the (P + M)/(A + T) ratio. In a study by Ćenanović and Duraković (2015), treatment of A. cepa root tip cells with 825 μg/ml of ascorbic acid induced a decrease in the frequency of cells in metaphase, anaphase and telophase while the frequency of cells in prophase increased significantly (p < 0.05). Decrease in the number of cells in telophase had no statistical significance.…”

Section: Discussionmentioning

confidence: 96%

Evaluation of Procavia capensis hyraceum used in traditional medicine for modulation of mutagen-induced genotoxicity

Asita¹,

Rantsâ€TMo²,

Magama³

et al. 2018

J. Med. Plants Res.

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Hyraceum (HM) is used in traditional medicine in Southern Africa. Three concentrations of HM (mg/ml in distilled water) (0.0156, 0.03125 and 0.0625) were assessed for cytotoxicity (CT), genotoxicity (GT) and modulation of cyclophosphamide (CP)-and ethyl methanesulfonate (EMS)-induced GT using the Allium cepa assay following 24 h treatment. CP (1.00 mg/ml) and EMS (0.0375 mg/ml) were not cytotoxic but genotoxic. HM (0.03125, 0.0625 mg/ml) and its mixtures with CP or EMS induced significant reduction (p <0.05) of the mitotic index (MI) and were adjudged cytotoxic. HM alone and its mixtures with CP or EMS induced statistically significant genotoxicity (p < 0.05). Mixture of HM (0.016 mg/ml) with CP was not significantly more genotoxic than CP alone (ME 0.57). Each mixture of HM (0.03125, 0.0625 mg/ml) with CP was insignificantly less genotoxic than CP alone with modulatory effect (ME) of -0.14 and -0.01, respectively, which suggested no interaction between HM and CP. Mixtures of HM with EMS induced positive and significant (>2-fold) MEs and each mixture was significantly (p < 0.05) more genotoxic than HM or EMS alone which indicated a synergistic interaction. Sticky chromosomes, chromosome laggards, chromosome fragments, anaphase and telophase bridges, binucleate interphase cells were observed.

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In Vivo Genotoxicity Testing Of Vitamin C And Naproxen Sodium Using Plant Bioassay

Cited by 3 publications

References 14 publications

L-Ascorbic acid modulates the cytotoxic and genotoxic effects of salinity in barley meristem cells by regulating mitotic activity and chromosomal aberrations

L-Ascorbic acid modulates the cytotoxic and genotoxic effects of salinity in barley meristem cells by regulating mitotic activity and chromosomal aberrations

Evaluation of naproxen-induced oxidative stress, hepatotoxicity and in-vivo genotoxicity in male Wistar rats

Evaluation of Procavia capensis hyraceum used in traditional medicine for modulation of mutagen-induced genotoxicity

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