In vivo hepatic differentiation of mesenchymal stem cells from human umbilical cord blood after transplantation into mice with liver injury

Yu, Jiong; Cao, Hong; Yang, Jinfeng; Pan, Qiaoling; Ma, Jing; Li, Jianzhou; Li, Yanyuan; Li, Jun; Wang, Yingjie; Li, Lanjuan

doi:10.1016/j.bbrc.2012.04.156

Cited by 41 publications

(28 citation statements)

References 34 publications

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“…Another type of stem cells, named adult or somatic stem cells, has a controlled behavior and holds great promise for cell therapy and regenerative medicine Lin et al, 2011;Allickson and Xiang, 2012). Adult stem cells harvested from BW, AT, and UCB have shown multi-potent capacity and can be induced to differentiate into functional hepatocytes (Banas et al, 2007;Chen et al, 2012;Yu et al, 2012). However, BW-and AT-derived stem cells cannot be easily obtained by practical, cost-effective methods while obtaining UCB stem cells is limited to the time of birth .…”

Section: Discussionmentioning

confidence: 99%

Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells

Mou

Lin

Chen

et al. 2013

J. Zhejiang Univ. Sci. B

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Orthotopic liver transplantation (OLT) is the only proven effective treatment for both end-stage and metabolic liver diseases. Hepatocyte transplantation is a promising alternative for OLT, but the lack of available donor livers has hampered its clinical application. Hepatocyte-like cells (HLCs) differentiated from many multi-potential stem cells can help repair damaged liver tissue. Yet almost suitable cells currently identified for human use are difficult to harvest and involve invasive procedures. Recently, a novel mesenchymal stem cell derived from human menstrual blood (MenSC) has been discovered and obtained easily and repeatedly. In this study, we examined whether the MenSCs are able to differentiate into functional HLCs in vitro. After three weeks of incubation in hepatogenic differentiation medium containing hepatocyte growth factor (HGF), fibroblast growth factor-4 (FGF-4), and oncostain M (OSM), cuboidal HLCs were observed, and cells also expressed hepatocyte-specific marker genes including albumin (ALB), α-fetoprotein (AFP), cytokeratin 18/19 (CK18/19), and cytochrome P450 1A1/3A4 (CYP1A1/3A4). Differentiated cells further demonstrated in vitro mature hepatocyte functions such as urea synthesis, glycogen storage, and indocyanine green (ICG) uptake. After intrasplenic transplantation into mice with 2/3 partial hepatectomy, the MenSC-derived HLCs were detected in recipient livers and expressed human ALB protein. We also showed that MenSC-derived HLC transplantation could restore the serum ALB level and significantly suppressed transaminase activity of liver injury animals. In conclusion, MenSCs may serve as an ideal, easily accessible source of material for tissue engineering and cell therapy of liver tissues.

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Section: Discussionmentioning

confidence: 99%

Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells

Mou

Lin

Chen

et al. 2013

J. Zhejiang Univ. Sci. B

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“…18,22,29,40 Tail A cell count of 5×10 5 suspended in 100µl PBS could be injected into the tail vein of rodents. 1,19,28 Portal vein Zhao and colleagues showed that intravenous injection of MSC is effective in the treatment of liver fibrosis in comparison with intrahepatic or intraperitoneal injection. As in intravenous injection liver lobules were normal, but in intrahepatic and intraperitoneal injection severe collagen deposition was observed 5 .…”

Section: Intrasplenicmentioning

confidence: 99%

“…A cell count of 1×10 6 suspended in 30µl of PBS or DMEM could be injected through the portal vein. 20,21,26 Homing and efficacy of cell therapy Histopathological methods such as Haematoxylin and Eosin (H&E), 22,28 Masson Trichrome 21,22 and Sirius Red 22 staining were used for detection of tissue structure changes after cell therapy in liver failure. The homing and engraftment of the transplanted cells to the target organ is key to the success of the cell therapy.…”

Section: Intrasplenicmentioning

confidence: 99%

“…18,19 Alu sequence detection by polymerase chain reaction (PCR) and green fluorescent protein (GFP) fusions are other useful techniques for tracking and locating transplanted cells. 9,19,21,28 Functional assay of transplanted cells Characterization of cell viability and cell identity after administering the cells is very important in the long-term follow-up study of liver cell therapy. Mortality rate is a reliable indicator of transplant functionality and overall recipient health.…”

Section: Tracing Of Transplanted Cellsmentioning

confidence: 99%

“…27 Mixture of Dgalactoseamine (D-gal) and lipopolysaccharide (LPS) 0.5 mg/g and 1 ng/g, respectively, in 1 ml of saline via intraperitoneal have also been used for inducing fulminant hepatic failure (FHF) in mice. 28 …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

Goradel¹,

Darabi²,

Shamsasenjan³

et al. 2015

Adv Pharm Bull

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The In Vivo Evaluation of the Therapeutic Potential of Human Adipose Tissue-Derived Mesenchymal Stem Cells for Acute Liver Disease

Katsuda¹,

Kurata

Tamai

et al. 2014

Methods in Molecular Biology

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Mesenchymal stem cells (MSCs) have emerged as an attractive candidate for cell therapy applications. In the prior decade, many animal studies have demonstrated that MSCs are therapeutically beneficial for the treatment of liver disease. The carbon tetrachloride (CCl4)-induced acute hepatitis model has been the most widely used model in these studies. Our group has utilized the CCl4-induced mouse hepatitis model to study the therapeutic potential of human adipose tissue-derived MSCs (hADSCs). We have demonstrated that systemically administered hADSCs engrafted into the damaged liver and promoted tissue repair. This phenomenon likely reflected the paracrine effects of the administered hADSCs. In this chapter, we describe a method to evaluate the therapeutic efficacy of the systemic administration of hADSCs in the CCl4-induced mouse model of acute hepatitis.

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In vivo hepatic differentiation of mesenchymal stem cells from human umbilical cord blood after transplantation into mice with liver injury

Cited by 41 publications

References 34 publications

Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells

Menstrual blood-derived mesenchymal stem cells differentiate into functional hepatocyte-like cells

Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

The In Vivo Evaluation of the Therapeutic Potential of Human Adipose Tissue-Derived Mesenchymal Stem Cells for Acute Liver Disease

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