In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve

Nassif, Nader; Cense, Barry; Park, B. H.; Pierce, Mark C.; Yun, Seok Hyun; Bouma, Brett E.; Tearney, Guillermo J.; Chen, T. C.; Boer, Johannes F. de

doi:10.1364/opex.12.000367

Cited by 495 publications

(363 citation statements)

References 18 publications

Supporting

Mentioning

355

Contrasting

Unclassified

Order By: Relevance

“…The problem remains for fast imaging (40 s integration time or less). Sensitivity in this case is not satisfactory, but can be attained by a more powerful light source and improvements in our spectrometer (for example spectrometer efficiency is 6% and should be higher, at least 20% to 30% [31,35]). CCD saturation powers at 40 s and 400 s are 60 W and 6 W respectively.…”

Section: Resultsmentioning

confidence: 96%

Common path Fourier domain optical coherence tomography based on multiple reflections within the sample arm

Krstajić

Hogg

Matcher

2011

Optics Communications

View full text Add to dashboard Cite

We present a common path Fourier domain optical coherence tomography (FDOCT) setup where the reference signal arises from multiple reflections within the sample arm. Two configurations are demonstrated. The first is based on a reflective microscope objective while the second is based on a normal (refractive) microscope objective. The second configuration is effectively a Mireau interferometer. We present sensitivity analysis of these setups and images of in vivo skin. Advantages of both common path arrangements include: 1) the reference surface is not close to the sample surface while keeping the optical path lengths matched (so the additional interferometer is not needed) and 2) the user can independently control reference and sample arm power. Additionally, the configuration using the refractive objective ensures that the coherence gate and focus gate always match. A disadvantage is that the reference arm power in certain circumstances is not optimal (i.e. close to saturating the CCD). However, this issue can be removed by a light source of sufficient output power. We believe the idea is scalable and therefore of interest to endoscopy applications. 3 RESEARCH HIGHLIGHTS• Multiple reflections within the sample arm can be beneficial.• We demonstrate a novel common-path Fourier domain OCT based on Mireau interferometer.

show abstract

Section: Resultsmentioning

confidence: 96%

Common path Fourier domain optical coherence tomography based on multiple reflections within the sample arm

Krstajić

Hogg

Matcher

2011

Optics Communications

View full text Add to dashboard Cite

show abstract

“…Also the employed scanning microscope prevented implementation of a shutter which would have been necessary for rapid automated background measurement in time-lapse imaging [5]. Fixed pattern suppression ac-cording to [22] (cf. Table 1(c), Figure 5(b)) and mean background subtraction [19] achieve a BSR increase of about 1 dB but both methods exhibit similar horizontal line artifacts (cf.…”

Section: Resultsmentioning

confidence: 99%

“…TVFP noise is not suppressed as indicated by white arrow. (b) fixed pattern suppression according to [22], cf. Table 1 The en face image using spectrum energy (Figure 6(d)) provides an image that allows easy judgment of the field of view of the scanning microscope.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Artefact reduction for cell migration visualization using spectral domain optical coherence tomography

Höfer¹,

Povazˇay²,

Hermann³

et al. 2011

Journal of Biophotonics

View full text Add to dashboard Cite

Visualization of cell migration during chemotaxis using spectral domain optical coherence tomography (OCT) requires non‐standard processing techniques. Stripe artefacts and camera noise floor present in OCT data prevent detailed computer‐assisted reconstruction and quantification of cell locomotion. Furthermore, imaging artefacts lead to unreliable results in automated texture based cell analysis. Here we characterize three pronounced artefacts that become visible when imaging sample structures with high dynamic range, e.g. cultured cells: (i) time‐varying fixed‐pattern noise; (ii) stripe artefacts generated by background estimation using tomogram averaging; (iii) image modulations due to spectral shaping. We evaluate techniques to minimize the above mentioned artefacts using an 800 nm optical coherence microscope. Effect of artefact reduction is shown exemplarily on two cell cultures, i.e. Dictyostelium on nitrocellulose substrate, and retinal ganglion cells (RGC‐5) cultured on a glass coverslip. Retinal imaging also profits from the proposed processing techniques. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

“…These artifacts result in a fixed noise pattern in A-scan signal, and periodic strip pattern noise in B-scan images. This noise contribution can be removed by reference spectrum subtraction [8]. However this method needs pre-processing which means precise system calibration and additional reference signal measurement.…”

Section: Introductionmentioning

confidence: 99%

Reduction of periodic noise in Fourier domain optical coherence tomography images by frequency domain filtering

Xie

Chen

Hofmann

2012

Biomedical Engineering / Biomedizinische Technik

View full text Add to dashboard Cite

In Fourier domain optical coherence tomography (FDOCT) coherence noise is caused by interference of light waves back-reflected or scattered from different spots within a measuring depth or from optical components within the OCT system such as a bended optical fibre. This coherence noise appears as periodic stripe patterns along the axial direction in resulting images. Background subtraction and physical segmentation are used to degrade the periodic coherence noise in previous studies. Here we utilise a post-processing method by applying a frequency domain filter to reduce the periodic coherence noise. Even though the approach taken is a very simple one, it visibly removes the periodic coherence noise from FDOCT images without influencing the original measured objects. The edges and textures of the objects in the processed image are identical as in the original image. The signal to noise ratio is also enhanced by using frequency domain filtering. Applying frequency domain filtering to FDOCT image can reduce the periodic noise significantly. This method is an easy, efficient way to improve the image quality without calibrating the system or measuring the background noise each time before a measurement.

show abstract

In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve

Cited by 495 publications

References 18 publications

Common path Fourier domain optical coherence tomography based on multiple reflections within the sample arm

Common path Fourier domain optical coherence tomography based on multiple reflections within the sample arm

Artefact reduction for cell migration visualization using spectral domain optical coherence tomography

Reduction of periodic noise in Fourier domain optical coherence tomography images by frequency domain filtering

Contact Info

Product

Resources

About