In-vivo, hypoglycemic, hypolipidemic and oxidative stress inhibitory activities of Myrtus communis L. fruits hydroalcoholic extract in normoglycemic and streptozotocin-induced diabetic rats

Taş, Sibel; Taş, Birol; Bassalat, Najlaa; Jaradat, Nidal

doi:10.4066/biomedicalresearch.29-18-708

Cited by 23 publications

(14 citation statements)

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“…In the present study, as well as in our previous ones (18,19,(29)(30)(31), we demonstrated oxidative stress in STZ-induced diabetic rats. Oxidative stress was evident by the increased plasma and tissue MDA levels accompanying alterations in the activities of antioxidant enzymes.…”

Section: Discussionsupporting

confidence: 87%

“…The fi ndings of various studies on the antioxidant activity in diabetes mellitus are contradictory. There are studies indicating SOD and GSH-Px activities as increased (19,20,31), decreased (44,45) or unaltered (46). We found that D group exhibited higher SOD and unchanged GSH-Px activity compared to the C group.…”

Section: Discussionmentioning

confidence: 45%

“…PON activity protects lipoproteins and tissues against oxidative modifi cation and it is well known that lipoprotein oxidation is one of the key mechanisms involved in the initiation and progression of atherosclerosis (17). We and others have previously reported that PON activity was reduced in diabetes mellitus (18)(19)(20). Decreased serum PON activity might be related to glycation and/or oxidative modifi cation of HDL and the enzyme paraoxonase (21).…”

Section: Introductionmentioning

confidence: 98%

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Effects of thiamine treatment on oxidative stress in experimental diabetes

Sarandöl¹,

Taş²,

Serdar³

et al. 2020

BLL

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AIM: Hyperglycemia, oxidative stress and hyperlipidemia are features of diabetes mellitus. Thiamine has benefi cial effects on carbohydrate metabolism and it was proposed that this vitamin has antihyperlipidemic and antioxidant effects. Our aim was to investigate the effects of thiamine on oxidative stress and metabolic changes in streptozotocin (STZ) induced diabetic rats. METHOD: Diabetes was induced by a single intraperitoneal injection of STZ. Thiamine (6 mg/kg) was added to drinking water for fi ve weeks. The rats were divided into four groups: control rats; thiamine treated control rats; diabetic rats; thiamine treated diabetic rats. Plasma and tissue malondialdehyde (MDA) levels were measured by high-performance liquid chromatography and spectrophotometry, respectively. Paraoxonase (PON) and arylesterase (AE) activities were measured with spectrophotometric methods, and erythrocyte superoxide dismutase (SOD) and blood glutathione peroxidase (GSH-Px) activities were determined using commercial kits. RESULTS: Thiamine treatment reduced plasma and tissue MDA levels, serum glucose, total cholesterol and triglyceride levels, and increased serum high density lipoprotein-cholesterol and insulin levels, serum PON and AE, erythrocyte SOD and blood GSH-Px activities. CONCLUSION: Thiamine signifi cantly improves oxidative stress and has hyperinsulinemic and antihyperlipidemic effects so we suggest that thiamine might be used as a supportive therapeutic agent in diabetes (Tab. 2, Fig. 3, Ref. 53).

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 45%

Section: Introductionmentioning

confidence: 98%

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Effects of thiamine treatment on oxidative stress in experimental diabetes

Sarandöl¹,

Taş²,

Serdar³

et al. 2020

BLL

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“…is damage could lead to various diseases over time, such as atherosclerosis, diabetes, inflammatory conditions, high blood pressure, heart diseases, cancer, neurodegenerative diseases, and aging. To avoid oxidative stress, one has to make sure he gets enough antioxidants in his diet, quit smoking, wear sunscreen, avoid overeating, get plenty of sleep, and do regular exercises [13].…”

Section: Introductionmentioning

confidence: 99%

Anticancer, Free Radicals, and Digestive Enzyme Inhibitory Activities of Rubus sanctus Schreb Root Four Solvent Fractions

Jaradat

Dwikat

Amer

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Humankind used herbal products as a source of medicines since they understood their therapeutic benefits from ancient times. Therefore, the current research aimed to determine the anticancer, antioxidant, and metabolic enzyme inhibitory activities of Rubus sanctus (RS) root four solvent fractions for the first time. Methods. The antioxidant, antilipase, and anti-α-amylase potentials of (RS) four solvent fractions were evaluated using standard biomedical assays. Moreover, the DNA cell cycle of liver cancer was assessed using a propidium iodide (PI) assay. At the same time, the apoptosis activity was estimated utilizing flow the cytometry method. Results. The methanol and acetone (RS) fractions showed the highest antioxidant activity with IC50 values of 0.078 ± 0.22 and 0.67 ± 0.25 μg/ml, respectively, compared with Trolox, which has an antioxidant IC50 value of 2.039 ± 0.52 μg/ml. Moreover, the methanol (RS) fraction has the highest anti-α-amylase activity with an IC50 value of 20.12 ± 0.34 μg/ml compared with acarbose, which has an IC50 value of 6.565 ± 0.3 μg/ml. Also, the acetone (RS) fraction revealed the highest antilipase activity with an IC50 value of 6.03 ± 1.23 μg/ml compared with the positive control orlistat which has an IC50 value of 0.39 ± 0.45 μg/ml. The aqueous, methanol, acetone, and hexane fractions of the (RS) roots decreased the secretion of the α-fetoprotein in the liver cancer cells. The acetone fraction was the most potent α-fetoprotein inhibitor with an average of 237 ± 12.5% compared with the average of the untreated cells, which was 4066.6 ± 202%. The hexane fraction was the most effective in diminishing apoptosis with an average of 14.5 ± 1.6%, compared with 49% ± 2 untreated cells’ average. In inhibiting cell cycle progression, it was recognized that methanol fraction seems to be the most powerful amplifier of the (RS) effect, as it increased the proportion of the cells with an average of 24.5 ± 2.2%, compared with 7.4 ± 1.8% in the doxorubicin (DOX). Data indicated a decrease in cell proliferation rate by prolonging the G2-M phase and thus slowing cancer progression. Our results suggest that (RS) roots four solvent fractions have potential anticancer activity. Conclusion. The (RS) roots four solvent fractions have potential anticancer, antioxidant, antilipase, and α-amylase inhibitory activities. It could be a promising source for applications in the functional food, nutraceutical, and pharmaceutical industries.

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“…Diyabetik sıçanlarda artmış MDA seviyesi ile birlikte antioksidan enzim ve moleküllerin azalmış aktivitesi muhtemelen oksidatif stres ve azalmış antioksidan savunma potansiyeli ile ilişkili olabilir. Yapılan bir araştırmada, hiperlipidemik, hiperglisemik komplikasyonları ile ilişkili oksidatif stres sonucunda indüklenmiş lipid peroksidasyonun hücre hasarlarına neden olduğu bildirilmiştir [38]. STZ'nin neden olduğu diyabetik sıçanlarda MDA seviyesinin yükselmesi ve antioksidan savunma unsurlarında önemli azalışlar (SOD, CAT, GSH gibi) benzer çalışmalarda rapor edilmiştir [39,40].…”

Section: Bulgular Ve Tartışmaunclassified

Streptozotosin kaynaklı diyabetik sıçanlarda Arum rupicola'nın in vivo antioksidan potansiyeli

Özok

Güneş

2019

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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Öz Diabetes mellitus, dünya popülasyonunun azımsanmayacak bir kesimini etkileyen metabolik bir hastalıktır. Arum rupicola, Doğu ve Güneydoğu Anadolu'da, halk hekimliğinde diyabetin tedavisi için kullanılmaktadır. Bu çalışma, Arum rupicola'nın streptozotosin (STZ) ile oluşturulan diyabetik sıçanlarda toprak üstü kök ve gövde liyoflize ekstresinin oksidatif stres belirteçleri üzerine etkisini değerlendirmek için tasarlanmıştır. Çalışmanın deneysel modellemesine göre, toplam 36 adet erkek sıçan rastgele altı gruba ayrıldı. İlk grup, normal kontrol (NK), ikinci grup, streptozotosin (STZ) ile deneysel diyabet oluşturuldu (DK). Üçüncü grup, diyabet+akarboz (DAK) deneme süresince verildi. Dört, beş ve altıncı gruplardaki sıçanlar STZ ile diyabet oluşturulduktan sonra sırasıyla 100, 200 ve 400 mg/kg (DAR: Diyabet+Arum ekstresi: DAR 100, DAR 200, DAR 400 mg/kg) doz aralığında Arum rupicola liyoflize ekstresi gavaj yolu sıçanlara verildi. Bu çalışmada, antioksidan savunma sistemi unsurlarından olan süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (CAT) aktivitesi ile non-enzimatik antioksidan olan glutatyon (GSH)'un düzeylerinin ölçümleri gerçekleştirildi. Ayrıca, lipit peroksidasyonun (LPO) son ürünü olan malondialdehit (MDA) seviyeside belirlendi. Diyabetik gruplarda, uygulama dozlarının tümünde SOD, GPx ve CAT ve GSH düzeylerindeki azalışlar normal kontrol (NK) grubuna göre anlamlı bulundu. Ancak eritrosit ve karaciğer dokularında lipit peroksidasyonu sonucu oluşan MDA'in düzeyinde görülen azalma normal kontrol ve diyabetik kontrol gruplarına göre anlamlı bulundu (p≤0.05). Diğer yandan, beyin dokusu DAR3 grubunda ve böbrek dokusu DAR1, DAR2, DAR3 gruplarında artan MDA düzeyleri istatiksel olarak anlamlı bulunmuştur. Bitki ekstresi uygulanan diyabetik gruplarda, farklı dokularda belirlenen değişken SOD, GPx ve CAT aktivite ve GSH'un ölçümleri istatistiksel olarak anlamlı bulundu (p≤0.05). Sonuç olarak, diyabetik sıçanlarda oksidatif stresin etkileri karşısında Arum rupicola ekstresinin doza bağlı dokudan-dokuya farklılık gösteren antioksidatif etkiye sahip olduğu tespit edilmiştir.

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In-vivo, hypoglycemic, hypolipidemic and oxidative stress inhibitory activities of Myrtus communis L. fruits hydroalcoholic extract in normoglycemic and streptozotocin-induced diabetic rats

Abstract: In-vivo, hypoglycemic, hypolipidemic and oxidative stress inhibitory activities of Myrtus communis L. fruits hydroalcoholic extract in normoglycemic and streptozotocin-induced diabetic rats.

Cited by 23 publications

References 50 publications

Effects of thiamine treatment on oxidative stress in experimental diabetes

Effects of thiamine treatment on oxidative stress in experimental diabetes

Anticancer, Free Radicals, and Digestive Enzyme Inhibitory Activities of Rubus sanctus Schreb Root Four Solvent Fractions

Streptozotosin kaynaklı diyabetik sıçanlarda Arum rupicola'nın in vivo antioksidan potansiyeli

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