In vivo identification of Bmp2-correlation networks during fracture healing by means of a limb-specific conditional inactivation of Bmp2

Yu, Yau-Hua; Wilk, Katarzyna; Waldon, PhiAnh; Intini, Giuseppe

doi:10.1016/j.bone.2018.07.016

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Bone morphogenetic protein-2 (BMP-2) is a powerful osteogenic factor that induces osteoblast differentiation and promotes bone formation [15]. Enhanced BMP-2 expression is known to play an essential role in the initiation of fracture healing [16]. We found that EGCG enhanced BMP-2 mRNA expression in human bone marrow derived mesenchymal stem cells (BMSCs) [17].…”

Section: Introductionmentioning

confidence: 98%

Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing

et al. 2020

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Green tea drinking can ameliorate postmenopausal osteoporosis by increasing the bone mineral density. (-)-Epigallocatechin-3-gallate (EGCG), the abundant and active compound of tea catechin, was proven to be able to reduce bone loss and ameliorate microarchitecture in female ovariectomized rats. EGCG can also enhance the osteogenic differentiation of murine bone marrow mesenchymal stem cells and inhibit the osteoclastogenesis in RAW264.7 cells by modulation of the receptor activator of nuclear factor-kB (RANK)/RANK ligand (RANKL)/osteoprotegrin (OPG) (RANK/RANKL/OPG) pathway. Our previous study also found that EGCG can promote bone defect healing in the distal femur partially via bone morphogenetic protein-2 (BMP-2). Considering the osteoinduction property of BMP-2, we hypothesized that EGCG could accelerate the bone healing process with an increased expression of BMP-2. In this manuscript, we studied whether the local use of EGCG can facilitate tibial fracture healing. Fifty-six 4-month-old rats were randomly assigned to two groups after being weight-matched: a control group with vehicle treatment (Ctrl) and a study group with 10 µmol/L, 40 µL, EGCG treatment (EGCG). Two days after the operation, the rats were treated daily with EGCG or vehicle by percutaneous local injection for 2 weeks. The application of EGCG enhanced callus formation by increasing the bone volume and subsequently improved the mechanical properties of the tibial bone, including the maximal load, break load, stiffness, and Young’s modulus. The results of the histology and BMP-2 immunohistochemistry staining showed that EGCG treatment accelerated the bone matrix formation and produced a stronger expression of BMP-2. Taken together, this study for the first time demonstrated that local treatment of EGCG can accelerate the fracture healing process at least partly via BMP-2.

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Section: Introductionmentioning

confidence: 98%

Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing

et al. 2020

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“…Additional potential applications of this procedure could include combining the direct drilling technique with the percutaneous delivery of BMPs, PRP or bone marrow within the drilled holes directly at the nonunion site. [26,27].…”

Section: Limitations and Prospectmentioning

confidence: 99%

Drilling percutaneously through the nonunion site: a more cost effective solution for long bone nonunions

Yin

Lü

Wang

et al. 2022

Preprint

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Background: Despite the development of improved fracture treatment, bone nonunion remains a challenge for orthopedic surgeons. Various therapies have been examined for the treatment of nonunions, including fixator exchange, bone grafting, application of growth factors, and even cell and gene therapy. However, all these methods have limitations, such as donor site complications, soft tissue problems, high cost and long hospitalization. This study aims to find a minimally invasive and cost-effective method for nonunion treatment. Methods: Between October 2018 and October 2019, nineteen patients with long bone nonunions (11 tibia and 8 femur) were treated in our trauma center. There were 14 males and 5 females and the average age was 56.1 years. Treatment of the nonunion was performed by a novel technique consisting of a direct drilling procedure. Under C-arm guidance, a k-wire was used to percutaneously drill across the nonunion site. The k-wire was directed along the longitudinal axis of the bone. The necrotic cortex of the nonunion site was drilled and the medullary cavity was re-established. The implants were maintained, as the fixation was stable. Regular rehabilitation and follow-up was performed until the patient achieved bone union. Results: 18 of the 19 cases achieved succesful bony union with this technique. Sixteen achieved union after a single drilling procedure, while two patients required a second drilling procedure to achieve union. One failure occurred in a female patients with a tibial shaft nonunion which only achieved union after revision fixation and cancellous bone grafting. The average union time after drilling technique was 7.4 month (range: 4-14), and the average hospital stay was 5.3 days (range: 4-15). Conclusion: This drilling technique provides a novel, minimally invasive, effective and low-cost method for the treatment of bone nonunion. It minimizes the iatrogenic damage and preserves the biological environment for fracture healing. The encouraging results of this technique warrants a larger study. Trial registration: Chinese Clinical Trial Registry ChiCTR-PPC-14005360. Registered 17 October 2014

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BMP2 induces osteogenic differentiation through ACKR3 in mesenchymal stem cells

Liu

Yao

Feng

et al. 2023

Biochemical and Biophysical Research Communications

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In vivo identification of Bmp2-correlation networks during fracture healing by means of a limb-specific conditional inactivation of Bmp2

Cited by 5 publications

References 39 publications

Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing

Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing

Drilling percutaneously through the nonunion site: a more cost effective solution for long bone nonunions

BMP2 induces osteogenic differentiation through ACKR3 in mesenchymal stem cells

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