In vivo imaging of EVs in zebrafish: New perspectives from “the waterside”

Verdi, Vincenzo; Bécot, Anaïs; Niel, Guillaume van; Verweij, Frederik J.

doi:10.1096/fba.2021-00081

Cited by 10 publications

(8 citation statements)

References 116 publications

(189 reference statements)

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“…And, a reciprocal EV-based crosstalk between muscle and bone has also been reported ( 317 ), highlighting the role of such vesicles, conserved among eukaryotes ( 318 ). Interestingly, zebrafish embryo-expressing fluorescent proteins associated with exosomes (e.g., CD63-phluorin) have been demonstrated to be a relevant model to study endogenous EVs in vivo to unravel fundamental aspects in EVs physiology and their role in inter-organ communication ( 319 , 320 ).…”

Section: New Approaches To Specific Analysis Of Tissue Interactionsmentioning

confidence: 99%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

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Control of tissue metabolism and growth involves interactions between organs, tissues, and cell types, mediated by cytokines or direct communication through cellular exchanges. Indeed, over the past decades, many peptides produced by adipose tissue, skeletal muscle and bone named adipokines, myokines and osteokines respectively, have been identified in mammals playing key roles in organ/tissue development and function. Some of them are released into the circulation acting as classical hormones, but they can also act locally showing autocrine/paracrine effects. In recent years, some of these cytokines have been identified in fish models of biomedical or agronomic interest. In this review, we will present their state of the art focusing on local actions and inter-tissue effects. Adipokines reported in fish adipocytes include adiponectin and leptin among others. We will focus on their structure characteristics, gene expression, receptors, and effects, in the adipose tissue itself, mainly regulating cell differentiation and metabolism, but in muscle and bone as target tissues too. Moreover, lipid metabolites, named lipokines, can also act as signaling molecules regulating metabolic homeostasis. Regarding myokines, the best documented in fish are myostatin and the insulin-like growth factors. This review summarizes their characteristics at a molecular level, and describes both, autocrine effects and interactions with adipose tissue and bone. Nonetheless, our understanding of the functions and mechanisms of action of many of these cytokines is still largely incomplete in fish, especially concerning osteokines (i.e., osteocalcin), whose potential cross talking roles remain to be elucidated. Furthermore, by using selective breeding or genetic tools, the formation of a specific tissue can be altered, highlighting the consequences on other tissues, and allowing the identification of communication signals. The specific effects of identified cytokines validated through in vitro models or in vivo trials will be described. Moreover, future scientific fronts (i.e., exosomes) and tools (i.e., co-cultures, organoids) for a better understanding of inter-organ crosstalk in fish will also be presented. As a final consideration, further identification of molecules involved in inter-tissue communication will open new avenues of knowledge in the control of fish homeostasis, as well as possible strategies to be applied in aquaculture or biomedicine.

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Section: New Approaches To Specific Analysis Of Tissue Interactionsmentioning

confidence: 99%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

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“…Cancer-derived EVs can also function as drones to remotely suppress T-cell function through PD1-PD-L1 binding. , They also enhance the pro-tumorigenic functions of macrophages, which help circulating cancer cells exit blood vessels. A recent study found that cancer cell-derived EVs would be taken up by endothelial cells and macrophages after being injected into zebrafish embryos, leading to the activation of pro-metastasis macrophages in vivo . , …”

Section: Role Of Evs In Cancer Progression and Metastasismentioning

confidence: 99%

Anticancer Therapy Targeting Cancer-Derived Extracellular Vesicles

Cheng,

Henick,

Cheng

2024

ACS Nano

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Extracellular vesicles (EVs) are natural lipid nanoparticles secreted by most types of cells. In malignant cancer, EVs derived from cancer cells contribute to its progression and metastasis by facilitating tumor growth and invasion, interfering with anticancer immunity, and establishing premetastasis niches in distant organs. In recent years, multiple strategies targeting cancer-derived EVs have been proposed to improve cancer patient outcomes, including inhibiting EV generation, disrupting EVs during trafficking, and blocking EV uptake by recipient cells. Developments in EV engineering also show promising results in harnessing cancer-derived EVs as anticancer agents. Here, we summarize the current understanding of the origin and functions of cancer-derived EVs and review the recent progress in anticancer therapy targeting these EVs.

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“…The study of endogenous EVs is tricky [ 69 ] and only partially translatable to mammalian models [ 70 , 71 ]; therefore, the most common approach for studying the biodistribution of EVs in vivo is injecting exogenous vesicles, isolated from conditioned cell culture media or biological fluids (plasma, urine, spinal fluid, saliva, etc.) [ 69 ].…”

Section: Molecular Imaging: a Tool To Study Ev Crosstalkmentioning

confidence: 99%

Extracellular Vesicles and Intercellular Communication: Challenges for In Vivo Molecular Imaging and Tracking

et al. 2023

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Extracellular vesicles (EVs) are a heterogeneous class of cell-derived membrane vesicles released by various cell types that serve as mediators of intercellular signaling. When released into circulation, EVs may convey their cargo and serve as intermediaries for intracellular communication, reaching nearby cells and possibly also distant organs. In cardiovascular biology, EVs released by activated or apoptotic endothelial cells (EC-EVs) disseminate biological information at short and long distances, contributing to the development and progression of cardiovascular disease and related disorders. The significance of EC-EVs as mediators of cell–cell communication has advanced, but a thorough knowledge of the role that intercommunication plays in healthy and vascular disease is still lacking. Most data on EVs derive from in vitro studies, but there are still little reliable data available on biodistribution and specific homing EVs in vivo tissues. Molecular imaging techniques for EVs are crucial to monitoring in vivo biodistribution and the homing of EVs and their communication networks both in basal and pathological circumstances. This narrative review provides an overview of EC–EVs, trying to highlight their role as messengers of cell–cell interaction in vascular homeostasis and disease, and describes emerging applications of various imaging modalities for EVs visualization in vivo.

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In vivo imaging of EVs in zebrafish: New perspectives from “the waterside”

Cited by 10 publications

References 116 publications

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

Anticancer Therapy Targeting Cancer-Derived Extracellular Vesicles

Extracellular Vesicles and Intercellular Communication: Challenges for In Vivo Molecular Imaging and Tracking

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