In vivo imaging of hepatic hemodynamics and light scattering property during ischemia-reperfusion in rats based on spectrocolorimetry

Abstract

Abstract:A red-green-blue camera-based imaging method is proposed for estimating spatial maps of concentrations of oxyhemoglobin (C HbO ), deoxyhemoglobin (C HbR ), total hemoglobin (C HbT ), tissue oxygen saturation (StO 2 ), and scattering power (b) in liver tissue. Hemodynamic responses to hepatic ischemia-reperfusion of in vivo rat liver tissues induced by portal triad occlusion were evaluated. Upon portal triad occlusion, this method yielded images of decreased C HbO , C HbT , StO 2 , and b, and increased C HbR followed by a progressive increase in C HbO and StO 2 during reperfusion. Time courses of the changes in C HbO , C HbR , C HbT , and StO 2 over different regions of interest (ROI s ) revealed that ischemia results in an abrupt significant (P<0.05) reduction in C HbO , C HbT , and StO 2 with a simultaneous increase in C HbR compared to the baseline level, indicative of the hemodynamic responses during hepatic ischemiareperfusion. Upon reperfusion, there was a gradual increase in C HbO and StO 2 , and decrease in C HbR . The change in average scattering power b implies the presence of morphological alterations in the cellular and subcellular structures induced by ischemia or anoxia. This study shows the potential of monitoring spatiotemporal changes in hemodynamic parameters and morphological changes in studies of hepatic pathophysiology. 31-39 (1976). 11. O. A. Trowell, "The experimental production of watery vacuolation of the liver," J. Physiol. 105(3), 268-297 (1946). 12. S. Akter, S. Maejima, S. Kawauchi, S. Sato, A. Hinoki, S. Aosasa, J. Yamamoto, and I. Nishidate, "Evaluation of light scattering and absorption properties of in vivo rat liver using a single-reflectance fiber probe during preischemia, ischemia-reperfusion, and postmortem," J. Biomed. Opt. 20(7), 076010 (2015). 13. M. Kretzschmar, A. Krüger, W. Schirrmeister, A. Krüger, and W. Schirrmeister, "Hepatic ischemia-reperfusion syndrome after partial liver resection (LR): hepatic venous oxygen saturation, enzyme pattern, reduced and oxidized glutathione, procalcitonin and interleukin-6," Exp. Toxicol. Pathol. 54(5-6), 423-431 (2003). 14. S. Marubayashi, M. Takenaka, K. Dohi, H. Ezaki, and T. Kawasaki, "Adenine nucleotide metabolism during hepatic ischemia and subsequent blood reflow periods and its relation to organ viability," Transplantation 30(4), 294-296 (1980). 15. W. Kamiike, M. Nakahara, K. Nakao, M. Koseki, T. Nishida, Y. Kawashima, F. Watanabe, and K. Tagawa, "Correlation between cellular ATP level and bile excretion in the rat liver," Transplantation 39(1), 50-55 (1985). 16. J. J. Lemasters, H. Bunzendahl, and R. G. Thurman, "Reperfusion injury to donor livers stored for transplantation," Liver Transpl. Surg. 1(2), 124-138 (1995). 17. J. W. Kupiec-Weglinski and R. W. Busuttil, "Ischemia and reperfusion injury in liver transplantation,"Transplant. Proc. 37(4), 1653-1656 (2005). In vivo label-free quantification of liver microcirculation using dual-modality microscopy," J.

Surgical spectral imaging

“…This has been reported in imaging experiments, following mechanical occlusion, on the skin ( Nishidate et al., 2013 ), bowel ( Clancy et al., 2015 ) and kidney ( Best et al., 2011 ). Similar induction of hypoxia has also been achieved in rodents by restricting the fraction of inspired oxygen ( Akter et al., 2017 ; Nishidate et al., 2018 ). Estimation of in-band noise and camera signal-to-noise ratio were made by Wirkert et al.…”

“…(2018) Snapshot Mosaic sensor 470–630 <15 16 512 × 256 90 (7.5 ms) Laparoscopy Kidney Nishidate et al. (2013) ; Akter et al. (2017) Snapshot RGB 450–690 – 25 1024 × 768 15 (~5 s) Open Skin, liver Jones et al.…”

Medical applications of reflectance spectroscopy in the diffusive and sub-diffusive regimes

“…We investigated in vivo evaluation of hepatic hemodynamics and tissue morphology on the basis of DRS using single reflectance optical fiber probe system, 35,36 and red, green, and blue (RGB) color imaging system. 64 Our DRS data analysis showed promising results in the use of this technology for diagnosing liver ischemia-reperfusion injury. Ischemia-reperfusion injury is a common consequence of liver surgery, particularly after liver transplantation due to occlusion of liver blood inflow, complicated by microcirculatory liver failure, followed by necrosis and cell death.…”

Single probe diffuse reflectance spectroscopy to assess the effect of sarcopoterium spinosum treatment on the cerebral tissue properties of ApoE knockout mouse

“…3) to recover the absolute intrinsic tissue absorption and reduced scattering coefficients. In the NIR spectral region, the reduced scattering coefficient, μ s' , for biological tissue obeys the power‐law function formulated as 45–47 : μ s' = A λ ‐ b where λ is the wavelength (nm), b is the scattering power shown to be related to mean size‐scale of the tissue features (membranes, nucleus, collagen, fibers, lysosomes, mitochondria, and other cell organelles in the tissue), and A is the scattering magnitude which correlates with the number, density, and distribution of these features, their radius, and the fluctuations in the medium refractive index 47–49 . Hence, structural changes of tissue cells and organelles on a macroscopic level can be studied via these two parameters.…”