In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish

Zou, Suqi; Hu, Bing

doi:10.1186/s13619-021-00079-3

Cited by 4 publications

(4 citation statements)

References 53 publications

(84 reference statements)

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“…Our analysis show that the visual OPCs ( olig2:EGFP /Sox2) are located in the POA at 2 dpf. Olig2 is a key transcription factor during oligodendrocyte differentiation (Zou & Hu, 2021 ) and Sox2 maintain stem cell properties (Wegner & Stolt, 2005 ). At 3 dpf, we can find olig2:EGFP /Sox2 cells also in the chiasmatic region of the ON where they start acquiring a more elongated morphology.…”

Section: Discussionmentioning

confidence: 99%

“…For example, oligodendrocytes expressing sox10 have been described to be involved in mouse regeneration (Mendonça et al., 2021 ). Zebrafish, an animal with continuous growth and where multiple de and remyelination events occur could be an ideal model for this type of research (Chung et al., 2013 ; Fang et al., 2014 ; Zou & Hu, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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Oligodendrocyte origin and development in the zebrafish visual system

Santos-Ledo

Pérez-Montes

DeOliveira-Mello

et al. 2022

J of Comparative Neurology

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Oligodendrocytes are the myelinating cells in the central nervous system. In birds and mammals, the oligodendrocyte progenitor cells (OPCs) originate in the preoptic area (POA) of the hypothalamus. However, it remains unclear in other vertebrates such as fish. Thus, we have studied the early progression of OPCs during zebrafish visual morphogenesis from 2 days post fertilization (dpf) until 11 dpf using the olig2:EGFP transgenic line; and we have analyzed the differential expression of transcription factors involved in oligodendrocyte differentiation: Sox2 (using immunohistochemistry) and Sox10 (using the transgenic line sox10:tagRFP). The first OPCs (olig2:EGFP/Sox2) were found at 2 dpf in the POA. From 3 dpf onwards, these olig2:EGFP/Sox2 cells migrate to the optic chiasm, where they invade the optic nerve (ON), extending toward the retina. At 5 dpf, olig2:EGFP/Sox2 cells in the ON also colocalize with sox10:tagRFP. When olig2:EGFP cells differentiate and present more projections, they become positive only for sox10:tagRFP. olig2:EGFP/sox10: tagRFP cells ensheath the ON by 5 dpf when they also become positive for a myelin marker, based on the mbpa:tagRFPt transgenic line. We also found olig2:EGFP cells in other regions of the visual system. In the central retina at 2 dpf, they are positive for Sox2 but later become restricted to the proliferative germinal zone without this marker. In the ventricular areas of the optic tectum, olig2:EGFP cells present Sox2 but arborized ones sox10:tagRFP instead. Our data matches with other models, where OPCs are specified in the POA and migrate to the ON through the optic chiasm.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oligodendrocyte origin and development in the zebrafish visual system

Santos-Ledo

Pérez-Montes

DeOliveira-Mello

et al. 2022

J of Comparative Neurology

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“…Based on soma shape and location, the glial cells observed in our Nissl preparations could be a type of oligodendrocytes or multipolar microgliocytes (Salvador‐Silva et al, 2000; Zou & Hu, 2021). Microglia performs a variety of functions in the fish retina, including defense against various harmful agents, scavenging cell debris, and regulation of nerve tissue homeostasis, growth, and regeneration (Huang et al, 2012; Salvador‐Silva et al, 2000).…”

Section: Discussionmentioning

confidence: 96%

“…Based on soma shape and location, the glial cells observed in our Nissl preparations could be a type of oligodendrocytes or multipolar microgliocytes (Salvador-Silva et al, 2000;Zou & Hu, 2021). Microglia…”

Section: Non-ganglion Cells In the Inner Retina Of The Pajama Cardina...mentioning

confidence: 93%

Retinal ganglion cell topography and spatial resolving power in the pajama cardinalfish Sphaeramia nematoptera (Bleeker, 1856)

Pushchin,

Aleskerov

2024

Journal of Fish Biology

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We studied the topography of retinal ganglion cells (GCs) and estimated spatial resolving power (SRP) in the pajama cardinalfish Sphaeramia nematoptera (Bleeker, 1856), a relatively small brightly colored fish inhabiting coral reefs and lagoons in the Western Pacific. S. nematoptera is an active night predator feeding on near‐bottom animal plankton and benthos. DAPI staining was used to label nuclei of GCs and non‐GCs in the inner plexiform and ganglion cell layers. Non‐GCs were distinguished from GCs in Nissl‐stained retinal wholemounts based on cell size, shape, and staining intensity. The proportion of displaced amacrine cells (DACs) varied from 15.46 ± 1.12 (visual streak [VS]) to 17.99 ± 1.06% (dorsal periphery) (mean ± S.E.M., N = 5); the respective proportions of glial cells were 6.61 ± 0.84 and 5.89 ± 0.76%. Thus, 76%–78% of cells in the ganglion cell layer and inner plexiform layer were GCs. The minimum spatial coverage of GCs (3600–4600 cells/mm2) was detected in the dorsal and ventral periphery. It gradually increased toward the central retina to form a moderate VS. The maximum GC density (11,400–12,400 cells/mm2) was registered in the central portion of the VS. No pronounced concentric retinal specializations were found. The total number of GCs ranged within 595.2–635.9 × 103. The anatomical spatial resolving power was minimum in the ventral periphery (4.91–5.53 cpd) and maximum in the central portion of the VS (8.47–9.07 cpd). The respective minimum separable angles were 0.18–0.20° and 0.11–0.12°. The relatively high spatial resolving power and presence of the VS in the pajama cardinalfish are in line with its highly visual behavior.

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Mechanism by which Rab5 promotes regeneration and functional recovery of zebrafish Mauthner axons

Cui,

Shen,

Song

et al. 2024

Neural Regeneration Research

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JOURNAL/nrgr/04.03/01300535-202506000-00031/figure1/v/2024-08-08T040853Z/r/image-tiff Rab5 is a GTPase protein that is involved in intracellular membrane trafficking. It functions by binding to various effector proteins and regulating cellular responses, including the formation of transport vesicles and their fusion with the cellular membrane. Rab5 has been reported to play an important role in the development of the zebrafish embryo; however, its role in axonal regeneration in the central nervous system remains unclear. In this study, we established a zebrafish Mauthner cell model of axonal injury using single-cell electroporation and two-photon axotomy techniques. We found that overexpression of Rab5 in single Mauthner cells promoted marked axonal regeneration and increased the number of intra-axonal transport vesicles. In contrast, treatment of zebrafish larvae with the Rab kinase inhibitor CID-1067700 markedly inhibited axonal regeneration in Mauthner cells. We also found that Rab5 activated phosphatidylinositol 3-kinase (PI3K) during axonal repair of Mauthner cells and promoted the recovery of zebrafish locomotor function. Additionally, rapamycin, an inhibitor of the mechanistic target of rapamycin downstream of PI3K, markedly hindered axonal regeneration. These findings suggest that Rab5 promotes the axonal regeneration of injured zebrafish Mauthner cells by activating the PI3K signaling pathway.

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In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish

Cited by 4 publications

References 53 publications

Oligodendrocyte origin and development in the zebrafish visual system

Oligodendrocyte origin and development in the zebrafish visual system

Retinal ganglion cell topography and spatial resolving power in the pajama cardinalfish Sphaeramia nematoptera (Bleeker, 1856)

Mechanism by which Rab5 promotes regeneration and functional recovery of zebrafish Mauthner axons

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