In Vivo Imaging Technology of Transplanted Stem Cells Using Quantum Dots for Regenerative Medicine

Yukawa, Hiroshi; Baba, Yoshinobu

doi:10.2116/analsci.17r005

Cited by 23 publications

(11 citation statements)

References 49 publications

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“…Gelatinbased StG gel that can clearly define the effect of the adhesion ligands enables reproducible analysis and control of differentiation of MSCs. Additionally, considering that clinical grade gelatin has also been developed, StG gel substrate for in vitro MSC analysis and preparation is, in combination with in vivo analytical technique such as in vivo imaging, 35 a great platform for MSCs studies and medical applications in the future.…”

Section: Relationship Between Runx2 Responsiveness To Substrate Elastmentioning

confidence: 99%

Designing Elastic Modulus of Cell Culture Substrate to Regulate YAP and RUNX2 Localization for Controlling Differentiation of Human Mesenchymal Stem Cells

et al. 2021

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To establish a guideline for the design of cell culture substrates to control human mesenchymal stem cell (MSC) differentiation, we quantitatively characterized the heterogeneity in the responsiveness of MSCs to the elastic modulus of culture substrates. We analyzed the elastic modulus-dependent dynamics of a mechanotransducer, YAP, and an osteogenic differentiation factor, RUNX2, in three different MSC lots using a styrenated gelatin gel with controllable elastic modulus. The percentage of cells with YAP in the nucleus increased linearly with increases in the elastic modulus, reaching a plateau at 10 kPa for all the lots analyzed. The increase in the percentage with the substrate elastic modulus was described by the same linear function. The percentage of cells with RUNX2 nuclear localization also increased linearly with increases in the substrate elastic modulus, plateauing at 5 kPa, although the regression lines to the linearly increasing regions varied between lots. These similarities and differences in YAP and RUNX2 dynamics among cell populations are basis to design the substrate elastic modulus to manipulate YAP and RUNX2 localizations.

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Section: Relationship Between Runx2 Responsiveness To Substrate Elastmentioning

confidence: 99%

Designing Elastic Modulus of Cell Culture Substrate to Regulate YAP and RUNX2 Localization for Controlling Differentiation of Human Mesenchymal Stem Cells

et al. 2021

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“…Third, exosomes are released with membrane-bound proteins that can be easily engineered to target a given tissue, including bone, and be incorporated into its cells [ 50 , 51 ]. Poor engraftment within the targeted tissue and accumulation of transplanted cells in nontargeted organs, mostly the lungs, are also issues in cell-based therapies [ 52 , 53 ]. Fourth, since exosomes are nanosized, they can navigate easier through tissue barriers, including the blood–brain barrier [ 54 ], which is very crucial when the target is within the central nervous system.…”

Section: Clinical Applicationsmentioning

confidence: 99%

Preparing the Bone Tissue Regeneration Ground by Exosomes: From Diagnosis to Therapy

2020

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Bone tissue engineering employs acellular scaffolds or scaffolds, along with cells and growth factors, to provide the mechanical support needed, as well as serve as a delivery vehicle for bioactive molecules to the injury sites. As tissue engineering continues to evolve, it has integrated two emerging fields: stem cells and nanotechnology. A paracrine factor that is found to be responsible for the major regenerative effect in stem cell transplantation is an extracellular vesicle called an ‘exosome’. Recent advances in nanotechnology have allowed the ‘exosome’ to be distinguished from other extracellular vesicles and be polymerized into a well-defined concept. Scientists are now investigating exosome uses in clinical applications. For bone-related diseases, exosomes are being explored as biomarkers for different bone pathologies. They are also being explored as a therapeutic agent where progenitor cell-derived exosomes are used to regenerate damaged bone tissue. In addition, exosomes are being tested as immune modulators for bone tissue inflammation, and finally as a delivery vehicle for therapeutic agents. This review discusses recently published literature on the clinical utilization of exosomes in bone-related applications and the correlated advantages. A particular focus will be placed on the potential utilization of regenerative cell-derived exosomes as a natural biomaterial for tissue regeneration.

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“…At each imaging time point, QDs and fluorophores are excited at predetermined wavelengths, and emit a fluorescent signal that can be used for imaging of transplanted cells. The QDs have narrow emission spectrum and broad excitation spectrum and have demonstrated high resolution, long duration, high sensitivity [30]. This strategy has been used for tracking of stem cells in many organs [18,28,29,31,32].…”

Section: Direct Labelingmentioning

confidence: 99%

Molecular Imaging of Stem Cells

Abbas

Gambhir

et al. 2019

STJ

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Regenerative medicine with the use of stem cells has appeared as a potential therapeutic alternative for many disease states. Despite initial enthusiasm, there has been relatively slow transition to clinical trials. In large part, numerous questions remain regarding the viability, biology and efficacy of transplanted stem cells in the living subject. The critical issues highlighted the importance of developing tools to assess these questions. Advances in molecular biology and imaging have allowed the successful non-invasive monitoring of transplanted stem cells in the living subject. Over the years these methodologies have been updated to assess not only the viability but also the biology of transplanted stem cells. In this review, different imaging strategies to study the viability and biology of transplanted stem cells are presented. Use of these strategies will be critical as the different regenerative therapies are being tested for clinical use.

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In Vivo Imaging Technology of Transplanted Stem Cells Using Quantum Dots for Regenerative Medicine

Cited by 23 publications

References 49 publications

Designing Elastic Modulus of Cell Culture Substrate to Regulate YAP and RUNX2 Localization for Controlling Differentiation of Human Mesenchymal Stem Cells

Designing Elastic Modulus of Cell Culture Substrate to Regulate YAP and RUNX2 Localization for Controlling Differentiation of Human Mesenchymal Stem Cells

Preparing the Bone Tissue Regeneration Ground by Exosomes: From Diagnosis to Therapy

Molecular Imaging of Stem Cells

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