In vivo imaging with SERS nanoprobes

Andreou, Chrysafis; Gregoriou, Yiota; Ali, Akbar; Pal, Soumik

doi:10.1016/b978-0-12-820548-8.00003-5

Cited by 3 publications

(3 citation statements)

References 137 publications

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“…6 SERS nanoparticles have been reported in preclinical studies to accumulate in tumors via passive targeting and to bind specific molecular targets when functionalized with antibodies or other targeting moieties. 5,7 As shown in Figure 1, SERS nanoparticles are typically composed of a gold core decorated with a Raman reporter molecule and encapsulated within a silica shell. Star-shaped nanoparticles have plasmonic resonance in the near-infrared (IR), enabling imaging with a 785 nm laser.…”

Section: Introductionmentioning

confidence: 99%

Detection and imaging of multiple immunotherapy markers in the tumor microenvironment with SERS nanoprobes for automated treatment response monitoring

Andreou,

Plakas,

Berisha

et al. 2024

Frontiers in Biological Detection: From Nanosensors to Systems XVI

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Detecting multiple molecular markers related to immune cells in vivo can revolutionize tumor phenotyping, monitor the effects of immunotherapy, and provide personalized treatment. Raman imaging with surface enhanced Raman scattering (SERS) nanoprobes is an optical imaging technique with high sensitivity and unparalleled multiplexing capacity. Here, we present a generalized methodology for the synthesis and visualization of SERS nanoprobes to detect multiple targets in vivo after intravenous administration in mouse models of cancer. A multiplexed imaging approach allows simultaneous scanning, unmixing, quantification, and visualization of the nanoprobes. We use this method to monitor the response of tumor-bearing mice (murine breast and colon cancer) to immune checkpoint blockade therapy. We visualize 8 immunological markers simultaneously, with subsequent immunohistological verification of each target. A spatially-aware multivariate approach is used for the segmentation of the acquired spectral images, which allowed their subsequent classification into responders or non-responders using machine learning.

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Section: Introductionmentioning

confidence: 99%

Detection and imaging of multiple immunotherapy markers in the tumor microenvironment with SERS nanoprobes for automated treatment response monitoring

Andreou,

Plakas,

Berisha

et al. 2024

Frontiers in Biological Detection: From Nanosensors to Systems XVI

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…5 Nanovehicles provide an innovative method of drug transport that offers protection from degradation, increases drug solubility, extends circulation time, delays drug clearance, and enhances tumor access via the enhanced permeability and retention (EPR) effect. 6,7 Polymeric nanoparticles have gained attention as cancer drug delivery vehicles due to their unique structural features but also due to the biocompatibility and biodegradability exhibited by the constituent polymers. 8 Amphiphilic polymers in aqueous solutions self-assemble into micellar structures with a hydrophilic shell and a hydrophobic core.…”

Section: Introductionmentioning

confidence: 99%