In-vivo Immunomodulatory Activities of Essential Oils of Artemisia abyssinica and Lepidium sativum in Mice

Ayenew, Kassahun; Ayalew, Amare; Wondmkun, Yehualashet; Tsige, Abate; Wolde, Addis; Sewale, Yihenew; Belihu, Gebremedhin; Fekade, Engidashet; Habteweld, Habtemariam

doi:10.2147/itt.s448317

Cited by 1 publication

(2 citation statements)

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“…Artemisia species have a wide range of uses in folk medicine and have been the subject of numerous chemical and biological studies . The genus has been reported to be rich in various secondary metabolites such as flavonoids, lignans, sesquiterpene lactones, coumarins, caffeoylquinic acids, acetylenes, and sterols, which possess various biological activities including diuretic, antimalarial, antimicrobial, antiviral, anthelmintic, anti-inflammatory, bronchodilator, hypolipidemic, antihypertensive, antioxidant, immunomodulatory, cytotoxic, antitumor, and laxative. , …”

Section: Introductionmentioning

confidence: 99%

“…2 Artemisia species have a wide range of uses in folk medicine and have been the subject of numerous chemical and biological studies. 3 The genus has been reported to be rich in various secondary metabolites such as flavonoids, lignans, sesquiterpene lactones, coumarins, caffeoylquinic acids, acetylenes, and sterols, 4 afford 12 subfractions, each 10 mL. Subfractions 8−10 were identified as compound 1 (53 mg).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial and Cytotoxicity of Extracts and Isolated Compounds from Artemisia abyssinica: A Combined Experimental and Computational Study

Tesfaye,

Endale,

Ramachandran

et al. 2024

ACS Omega

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Artemisia abyssinica is a widely cultivated hedge plant in Ethiopia. Traditionally, they have been used to treat a variety of health conditions, including intestinal problems, infectious diseases, tonsillitis, and leishmaniasis. Silica gel chromatographic separation of the methanol and ethyl acetate extracts of the leaves, roots, and stem barks of A. abyssinica led to the isolation of 12 compounds, labeled as 1−12. Among these, compounds 1, 3, 4, 5, and 7−11 are reported as new to the genus Artemisia. The extracts and isolated compounds from A. abyssinica were evaluated for their in vitro antibacterial activity against four bacterial strains: Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, using the disc diffusion assay. All of the extracts displayed weak antibacterial activity, with inhibition zone diameters (IZDs) ranging from 6.10 ± 0.3 to 9.30 ± 0.20 mm. The isolated compounds, on the other hand, exhibited weak to moderate antibacterial activity, with IZDs ranging from 6.00 ± 0.300 to 13.50 ± 0.50 mm. The most potent antibacterial activity was observed for compound 6, which showed an IZD of 13.30 ± 0.50 mm against E. coli and 13.50 ± 0.50 mm against P. aeruginosa. This activity was comparable to that of the positive control ceftriaxone, which had IZDs of 14.1 ± 0.3 and 13.8 ± 0.5 mm against E. coli and P. aeruginosa, respectively. The in silico molecular docking analysis against DNA gyrase B revealed that compound 5 showed a higher binding affinity (−6.9 kcal/mol), followed by compound 10 (−6.7 kcal/mol) and compound 12 (−6.3 kcal/mol), whereas ciprofloxacin showed −7.3 kcal/mol. The binding affinities of compounds 5, 11, 10, and 9 were found to be −5.0, −4.3, −4.2, and −4.0 kcal/mol against S. aureus Pyruvate kinase, respectively, whereas ciprofloxacin showed a binding affinity of −4.9 kcal/mol, suggesting that compound 5 had a better binding affinity compared with ciprofloxacin. The effect of extracts of A. abyssinica was evaluated for cytotoxic activity against the breast cancer cell line (MCF-7) by the MTT assay. The extracts induced a decrease in cell viability and exerted a cytotoxic effect at a concentration of 20 μg/mL. The highest percent cell viability was observed for the methanol extract of the stem (92.9%), whereas the least was observed for the methanol extract of the root (34.5%). The result of the latter was significant compared with the positive control. The binding affinities of the isolated compounds were also assessed against human topoisomerase inhibitors IIβ. Results showed that compound 5 showed a binding affinity of −6.0 kcal/mol, followed by 11 (−5.4 kcal/mol), 10 (−5.0 kcal/mol), and 11 (−4.9 kcal/mol). Similar to ciprofloxacin, compounds 4, 5, 6, 9, 10, and 12 comply with Lipinski's rule of five. Overall, the comprehensive investigation of the chemical constituents and their biological activities reinforces the traditional medicinal applications of A. abyssinica and warrants further exploration of this plant as a source of novel therapeut...

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