In Vivo, In Situ Tissue Analysis Using Rapid Evaporative Ionization Mass Spectrometry

Abstract

Beam me up: A novel mass spectrometric ionization technique based on rapid evaporation of biological tissues (see picture) can be used to analyze vital tissues during surgical intervention as well as for processed tissue specimens. A tissue identification system based on principal‐component analysis was developed. The method differentiates malignant tumor cells from the surrounding healthy tissue.

Discrimination of Human Astrocytoma Subtypes by Lipid Analysis Using Desorption Electrospray Ionization Imaging Mass Spectrometry

“…[8] A well-defined tumor margin boundary correlated with histopathological examination. Related ambient ionization methods have been used for similar purposes including 3D tissue imaging [9] and for characterization of lipids in surgical smoke during animal surgery [10].…”

XMS: Cross-Platform Normalization Method for Multimodal Mass Spectrometric Tissue Profiling

“…The DESI ionization mechanism comprises analyte extraction from the tissue surface followed by multiply charged droplets undergoing an electrospray-like ionization scenario [32,33]. In contrast, in the case of REIMS the tissue itself is converted into an aerosol by Joule-heating [34]. The major differences in the spectra are attributable to the intrinsic thermal degradation occurring during REIMS process Significant overlap within the chemical species detected by each ionization technique is the basis for successful crossplatform normalization.…”

Is the Pen really Mightier than the Scalpel?

“…But the technology relies on tissue damage to produce molecular ions. [2][3][4] Along with this, the major concern is that the electrosurgical smoke contains irritants, carcinogens, and neurotoxic compounds. Exposure to these compounds may cause health hazards.…”