In vivo investigation of homocysteine metabolism to polyamines by high-resolution accurate mass spectrometry and stable isotope labeling

Ruseva, Silviya; Lozanov, Valentin; Markova, P.; Girchev, R; Mitev, Vanio

doi:10.1016/j.ab.2014.04.007

Cited by 9 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the mild endotoxaemia induced in the current sheep did not lead to altered cysteine ILR, the response to supplemental methionine was reduced during the LPS challenge. Catabolism of methionine would not only increase intracellular cysteine for synthesis of glutathione but is linked to synthesis of spermine and spermidine ( 44 ) , polyamines that are key components during cell proliferation ( 45 ) , which may be needed to support the increased cell division when villi height increases ( 10 ) . Nonetheless, in other studies where gut-related responses to LPS have been observed these have usually involved an enteral challenge or use of live bacteria.…”

Section: Discussionmentioning

confidence: 99%

Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

et al. 2015

View full text Add to dashboard Cite

Some effects of parasitism, endotoxaemia or sepsis can be mitigated by provision of extra protein. Supplemented protein may encompass a metabolic requirement for specific amino acids (AA). The current study investigates a method to identify and quantify the amounts of AA required during inflammation induced by an endotoxin challenge. One of each pair of six twin sheep was infused in the jugular vein for 20 h with either saline (control) or lipopolysaccharide (LPS, 2 ng/kg body weight per min) from Escherichia coli. Between 12 and 20 h a mixture of stable isotope-labelled AA was infused to measure irreversible loss rates. From 16 to 20 h all sheep were supplemented with a mixture of unlabelled AA infused intravenously. Blood samples were taken before the start of infusions, and then continuously over intervals between 14 and 20 h. At 20 h the sheep were euthanised, and liver and kidney samples were taken for measurement of serine-threonine dehydratase (SDH) activity. LPS infusion decreased plasma concentrations of most AA (P < 0·05; P < 0·10 for leucine and tryptophan), except for phenylalanine (which increased P = 0·022) and tyrosine. On the basis of the incremental response to the supplemental AA, arginine, aspartate, cysteine, glutamate, lysine (tendency only), glycine, methionine, proline, serine and threonine were important in the metabolic response to the endotoxaemia. The AA infusion between 16 and 20 h restored the plasma concentrations in the LPS-treated sheep for the majority of AA, except for glutamine, isoleucine, methionine, serine and valine. LPS treatment increased (P < 0·02) SDH activity in both liver and kidney. The approach allows quantification of key AA required during challenge situations.Key words: Endotoxin lipopolysaccharide: Amino acids: Irreversible loss rate: Serine-threonine dehydratase: SheepThe protein and amino acid (AA) demands of animals for normal physiological states (e.g. growth, pregnancy and lactation) are now well characterised, especially for pigs and poultry (1) and with progress made in ruminants (2) . These requirements are altered, however, in response to injury, infection and parasitic challenge. In many cases, impact of these challenges can be ameliorated and the rate of recovery improved by provision of additional protein in the diet (3)(4)(5)(6) . Often, however, the amount of protein required is not known, with consequences for both animal performance and farm economics.More importantly, protein per se may not be required, but instead the metabolic responses to the challenge may increase the demand for specific AA. For example, practical and theoretical arguments have been presented for additional arginine in cardiovascular and pulmonary disorders (7)(8)(9)(10) , for cysteine (11) , leucine (12) and threonine (13) in sepsis, for glutamine in endotoxaemia (10) , and for phenylalanine to support acutephase protein synthesis (14) . In other situations, combinations of AA have been proposed (10,15,16) . Obviously different stages of injury, infection or recover...

show abstract

Section: Discussionmentioning

confidence: 99%

Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

et al. 2015

View full text Add to dashboard Cite

show abstract

“…[72] изучалось влияние гомоцистеина на биосин-тез полиаминов. Установлено, что d8-меченые ами-нопропильные фрагменты включаются в метионин и далее в спермидин и спермин во всех тканях.…”

Section: система полиаминов и ее биологическая рольunclassified

Homocysteine and Polyamines: Metabolic Relationship and Its Clinical Significance in Folate Inhibitors Chemotherapy

Неборак¹,

Лебедева²,

Головня³

et al. 2017

Oncopediatria

View full text Add to dashboard Cite

“…HCYS is biosynthesized only in cells, from methionine. 13 Methionine is an essential amino acid, while cysteine is biosynthesized by transfering a sulfur atom from methionine to the hydroxyl group of serine. Through the action of methionine-adenosyl-transferase on methionine, S-adenosyl methionine is produced, which through the intervention of methyl-transferase produces S-adenosyl-HCYS and a free methyl group (-CH 3 ).…”

Section: Hcys Metabolismmentioning

confidence: 99%

The Effects of Homocysteine Level in the Critically Ill Patient. A Review

Bedreag

Rogobete

Săndesc

et al. 2016

Journal of Interdisciplinary Medicine

View full text Add to dashboard Cite

Increased levels of homocysteine (HCYS) represent a risk factor for a series of physiopathological conditions: mental retardation, cardiovascular and neurodegenerative diseases, Parkinson's and Alzheimer's disease, depression, osteoporosis, endothelial dysfunction and inhibition of cell proliferation. This paper aims to present the pathophysiological implications of HCYS and the correlation of hyperhomocysteinemia (H-HCYS) with critical condition in the intensive care unit (ICU). Hypovitaminosis B and folate deficiency is directly involved in the inhibition of HCYS metabolism and the accumulation of HCYS in the plasma and tissues. Critically ill patients are more prone to H-HCYS due to hypermetabolism and accelerated synthesis produced by reactive oxygen species (ROS). In conclusion it can be affirmed that the determination and monitoring of HCYS plasma levels may be of interest in optimizing the therapy for critically ill patients. Moreover, by controlling HCYS levels, and implicitly the essential cofactors that intervene in the specific biochemical pathways, such as vitamin B6, vitamin B12 and folic acid can provide a diversified and personalized treatment for each patient.

show abstract

In vivo investigation of homocysteine metabolism to polyamines by high-resolution accurate mass spectrometry and stable isotope labeling

Cited by 9 publications

References 18 publications

Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

Homocysteine and Polyamines: Metabolic Relationship and Its Clinical Significance in Folate Inhibitors Chemotherapy

The Effects of Homocysteine Level in the Critically Ill Patient. A Review

Contact Info

Product

Resources

About