In vivo iron mobilisation evaluation of hydroxypyridinones in 59Fe-ferritin-loaded rat model

Liu, Zu Dong; Lu, Shu L.; Hider, Robert C.

doi:10.1016/s0006-2952(98)00319-0

Cited by 22 publications

(31 citation statements)

References 13 publications

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“…This was undertaken as previously described (16). The ferritin concentration from normal rats was approximately 380 g/ml (iron content approximately 1020 atoms iron/ ferritin) and the protein concentration from the iron overloaded rats was 500 g/ml (iron content approximately 1400 atoms iron/ferritin).…”

Section: Ferritinmentioning

confidence: 99%

Quantification of Non-Transferrin-Bound Iron in the Presence of Unsaturated Transferrin

Gosriwatana

Loréal

et al. 1999

Analytical Biochemistry

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159

145

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Section: Ferritinmentioning

confidence: 99%

Quantification of Non-Transferrin-Bound Iron in the Presence of Unsaturated Transferrin

Gosriwatana

Loréal

et al. 1999

Analytical Biochemistry

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159

145

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“…Iron was quantified in crude and partially purified protein fractions by modifying the colorimetric method developed by Liu et al (24). All solutions were made with Milli-Q water.…”

Section: Metal Analysismentioning

confidence: 99%

Purification and Properties of a Folate-catabolizing Enzyme

Suh

Oppenheim

Girgis

et al. 2000

Journal of Biological Chemistry

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We have identified and purified to homogeneity an enzyme from rat liver that catalyzes the oxidative catabolism of 5-formyltetrahydrofolate to p-aminobenzoylglutamate and a pterin derivative. Purification of the enzyme utilized six column matrices, including a pterin-6-carboxylic acid affinity column. Treatment of crude rat liver extracts with EDTA or heat decreased the specific activity of the enzyme by up to 85%. Peptides generated from the purified protein were sequenced and found to be identical to primary sequences present within rat light chain or heavy chain ferritin. Commercial rat ferritin did not display catabolic activity, but activity could be acquired with iron loading. The purified enzyme contained 2000 atoms of iron/ferritin 24-mer and displayed similar electrophoretic properties as commercial rat liver ferritin. The ferritin-catalyzed reaction displayed burst kinetics, and the enzyme catalyzed only a single turnover in vitro. Expression of rat heavy chain ferritin cDNA resulted in increased rates of folate turnover in cultured Chinese hamster ovary cells and human mammary carcinoma cells and reduced intracellular folate concentrations in Chinese hamster ovary cells. These results indicate that ferritin catalyzes folate turnover in vitro and in vivo and may be an important factor in regulating intracellular folate concentrations.

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“…The final red precipitate was dissolved in 0.05 m phosphate buffer (pH 7.4) and stored at 4°C. The radioactivity of 59 Fe‐ferritin was measured using a gamma counter (1282 Compugamma GS, Pickmere, Cheshire, UK), LKB Wallac (LKB wallac, Coventry, West Midlands, UK) [23]…”

Section: Methodsmentioning

confidence: 99%

“…The investigation was terminated 24 h after 59 Fe‐ferritin administration; the rats were killed by cervical dislocation, the liver and gastrointestinal tract (including its contents and the faeces) being removed for gamma counting. The calculations for ‘Iron mobilisation’, ‘Efficacy’ and ‘Total recovery’ are represented by equations , and [23]

\begin{matrix} left \\ Iron mobilisation (%) \\ = \frac{^{59} Fe - {Activity}_{(Gut and Faeces)} \times 100 %}{^{59} Fe - {Activity}_{(Gut and Faeces)} +^{59} Fe - {Activity}_{(Liver)}} \end{matrix}

Efficacy (%) = Iron mobilisation (%) - Control (%)

\begin{matrix} left \\ Total recovery (%) \\ = \frac{^{59} Fe - {Activity}_{(Gut and Faeces)} +^{59} Fe - {Activity}_{(Liver)} \times 100 %}{^{59} Fe - {Activity}_{(Dose)}} \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

Characterisation of a novel oral iron chelator: 1-(N-Acetyl-6-Aminohexyl)-3-Hydroxy-2-Methylpyridin-4-one

Pangjit

Banjerdpongchai

Phisalaphong

et al. 2015

Journal of Pharmacy and Pharmacology

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In vivo iron mobilisation evaluation of hydroxypyridinones in 59Fe-ferritin-loaded rat model

Cited by 22 publications

References 13 publications

Quantification of Non-Transferrin-Bound Iron in the Presence of Unsaturated Transferrin

Quantification of Non-Transferrin-Bound Iron in the Presence of Unsaturated Transferrin

Purification and Properties of a Folate-catabolizing Enzyme

Characterisation of a novel oral iron chelator: 1-(N-Acetyl-6-Aminohexyl)-3-Hydroxy-2-Methylpyridin-4-one

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