In vivo labeling of serotonin-containing neurons by 5,7-dihydroxytryptamine inAplysia

Jahan‐Parwar, Behrus; S.-Rózsa, Katalin; Salánki, J.; Evans, Marcus; Carpenter, David O.

doi:10.1016/0006-8993(87)90438-0

Cited by 35 publications

(24 citation statements)

References 24 publications

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“…Clusters of SLI cells in the visceral and right parietal ganglia lie near the border between the two ganglia, and similar localizations for these cells have been observed in other species (Longley and Longley 1986;Jahan-Parwar et al 1987;Hawkins 1989;S.-Rozsa et al 1986;Audesirk 1985;Kemenes et al 1989;Hatakeyama and Ito 1999). The counterpart of Pe8 has been reported in the pedal ganglia in Helix (S.-Rozsa et al 1986) and Lymnaea (Audesirk 1985;Kemenes et al 1989;Hatakeyama and Ito 1999), but not in Aplysia (Ono and McCaman 1984;Longley and Longley 1986;Jahan-Parwar et al 1987;Hawkins 1989).…”

Section: Discussionsupporting

confidence: 51%

“…On the anterior surface of the cerebral ganglion, the counterpart of Ce1 has been observed in all of the gastropod molluscs above (Longley and Longley 1986;Jahan-Parwar et al 1987;Hawkins 1989;S.-Rozsa et al 1986;Kemenes et al 1989), although one more cluster has been demonstrated in Helix (S.- Rozsa et al 1986) and three more clusters have been seen in Lymnaea (Kemenes et al 1989). On the posterior surface of the cerebral ganglion, putative counterparts of Ce2, 3, 4 and 5 have been observed in Aplysia (Longley and Longley 1986;Hawkins 1989).…”

Section: Discussionmentioning

confidence: 91%

“…Maps of serotonin-containing neurons are available for several species of gastropod mollusc (Aplysia: Ono and McCaman 1984;Longley and Longley 1986;Jahan-Parwar et al 1987;Hawkins 1989;Helix pomatia: S.-Rozsa et al 1986; Lymnaea stagnalis: Audesirk 1985; Kemenes et al 1989;Hatakeyama and Ito 1999). Generally speaking, the distribution of SLI cells in Limax appears to be similar to those in the species mentioned above.…”

Section: Discussionmentioning

confidence: 99%

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Distribution of serotonin-like immunoreactive neurons in the slug Limax valentianus

Shirahata

Watanabe

Kirino

2004

Cell and Tissue Research

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Immunohistochemical techniques were used to study the distribution of serotonin-containing neurons in the nervous system of the slug Limax valentianus. Approximately 350 serotonin-like immunoreactive cell bodies were found in the central nervous system. These were located in the cerebral, pedal, visceral and right parietal ganglia. Most serotonin-like immunoreactive neurons had small cell bodies, which were aggregated into discrete clusters. A pair of previously identified metacerebral giant cells were found on the anterior side of the cerebral ganglion, and two additional pairs of uniquely identifiable, serotonin-like immunoreactive cells were found on the posterior side of the cerebral ganglion. The whole-mount maps of these stained neurons will be useful in further physiological and biochemical studies of olfactory learning at the cellular level in Limax valentianus.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Distribution of serotonin-like immunoreactive neurons in the slug Limax valentianus

Shirahata

Watanabe

Kirino

2004

Cell and Tissue Research

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“…Still, these experiments allow us to further define the locus of 5-HT activity in the larval nervous system. 5-HT plays numerous roles in molluscan nervous systems (Walker, 1986) and major groups of serotonergic neurons have been identified in several gastropod taxa (Koshtoyants et al, 1961;Walker 1986;Jahan-Parwar et al, 1987;Goldberg and Kater, 1989;Diefenbach and Goldberg, 1990;McPherson and Blankenship, 1991;Fitzgerald and Carew, 1991). For example, 5-HT regulates neurite extension and growth-cone motility in the freshwater snail Helisoma trivolvis (Price and Goldberg, 1993); stimulates motor neurons in Aplysia california (Ram et al, 1991); plays an important role in molluscan peripheral tissues, especially the heart (Walker, 1986); and heightens motor activity in embryonic gastropods in a developmentally sensitive manner (Koshtoyants et al, 1961;Diefenbach et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Serotonin Injections Induce Metamorphosis in Larvae of the Gastropod Mollusc Ilyanassa obsoleta

Couper

Leise

1996

The Biological Bulletin

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Abstract:Bath-applied serotonin (5-HT) induces competent larvae of the marine snail Ilyanassa obsoleta to metamorphose. Previously, the mode of action of 5-HT, whether as an external ligand or as an internal neurotransmitter, was unknown. Larvae were injected with 10 -4 M 5-HT and other pharmacological agents to provide evidence that serotonergic neurons are necessary for metamorphosis in Ilyanassa larvae and that serotonin functions as a neurotransmitter or neuromodulator during this process. About 50% of 5-HT-injected animals metamorphose within 48 hours. Fluoxetine, a 5-HT re-uptake inhibitor, and alpha-methy1-5-hydroxytryptamine (αm5HT), a 5-HT agonist, were also effective inducers of metamorphosis. Gramine (3-[dimethyl-aminomethyl]indole), a 5-HT antagonist, inhibited the inductive activity of 5-HT, while the amino acid gamma-aminobutyric acid (GABA) resulted in rates of morphological restructuring similar to those of controls. Collectively, the results of our experiments support the idea that serotonergic neurons are active during larval metamorphosis of Ilyanassa and that 5-HT does not induce metamorphosis by binding to epidermal chemoreceptors.

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“…In one experiment, we stained serotonergic neurons in living animals using 5,7-dihydroxytryptamine (5,7-DHT) (Jahan-Parwar et al, 1987;Marinesco et al, 2004a). For this purpose, animals were injected twice with 10 mg/kg 5,7-DHT dissolved at 5 mg/ml in ASW, at a 1 week interval.…”

Section: Methodsmentioning

confidence: 99%

Regulation of Behavioral and Synaptic Plasticity by Serotonin Release within Local Modulatory Fields in the CNS ofAplysia

Marinesco¹,

Wickremasinghe²,

Carew³

2006

J. Neurosci.

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In Aplysia, serotonergic neurons are widely activated during sensitization training, but the effects of exogenous serotonin (5-HT) on reflex circuits vary, inducing short-or long-term synaptic facilitation or synaptic inhibition, depending on the site of application. During learning, it is possible that specific spatial patterns of 5-HT release evoked by training may produce different phases of sensitization or behavioral inhibition. To test this hypothesis, we examined the modulation of the tail-induced siphon withdrawal reflex by repeated noxious stimuli applied to one of three sites: the (1) ipsilateral or (2) contralateral sides of the tail or (3) the head. Ipsilateral tail shock produced long-term sensitization, whereas contralateral tail shock induced only short-term sensitization, and head shock produced inhibition. In parallel cellular experiments, tail-nerve shock evoked large 5-HT release localized around the ipsilateral tail sensory neurons (SNs) and motor neurons (MNs) but only modest 5-HT release in the contralateral pleural-pedal ganglia and in the abdominal ganglion, in which the siphon MNs are located. Head-nerve shock, in contrast, produced only modest 5-HT release in the pleural, pedal, and abdominal ganglia. Thus, each training protocol evoked a specific pattern of 5-HT release within the CNS. In addition, we found that 5-HT released in the pleural ganglia was correlated with facilitation of SN-MN synapses; however, in the abdominal ganglion, it was associated with inhibition of the synapses between identified interneurons (L29s) and siphon MNs (LFSs). Because 5-HT differentially modulates synaptic efficacy at different synaptic sites, our data can explain how specific spatial patterns of 5-HT release in local modulatory fields can contribute to the induction of short-or long-term sensitization or to behavioral inhibition.

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In vivo labeling of serotonin-containing neurons by 5,7-dihydroxytryptamine inAplysia

Cited by 35 publications

References 24 publications

Distribution of serotonin-like immunoreactive neurons in the slug Limax valentianus

Distribution of serotonin-like immunoreactive neurons in the slug Limax valentianus

Serotonin Injections Induce Metamorphosis in Larvae of the Gastropod Mollusc Ilyanassa obsoleta

Regulation of Behavioral and Synaptic Plasticity by Serotonin Release within Local Modulatory Fields in the CNS ofAplysia

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