In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive

Gimeno, Federico; Gatto, S.; Croxatto, J. Oscar; Ferro, José; Gallo, Juan E.

doi:10.1038/eye.2009.49

Cited by 9 publications

(8 citation statements)

References 25 publications

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“…20 However, Luengo Gimeno et al have found this number comparable with control. 28 Apoptosis of keratocytes and myofibroblasts is important in the initiation, modulation and termination of the corneal wound healing response following corneal injury, infections and surgery. 1 It was suggested that interleukin-1 29 released from injured epithelial cells induces keratocyte apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Platelet-Rich Plasma Prolongs Myofibroblast Accumulation in Corneal Stroma with Incisional Wound

Koulikovska

Szymanowski

Lagali

2015

Current Eye Research

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Purpose: The purpose of this study was to determine whether platelet-rich plasma (PRP) has an effect on corneal stromal cells in a rat model of wound healing following corneal incision. Materials and Methods: The effect of PRP on corneal wound healing in vivo was investigated in a corneal incision wound model in rats. 40 rats were wounded by deep corneal incision, and treated with either topically administered PRP (20 rats) or sodium chloride (20 rats). At 4 h and 1, 3, and 5 days after incision, a-smooth muscle actin (a SMA), SMAD2 and SMAD3 expression and apoptosis in stromal cells were evaluated by immunohistochemistry, and IL-1b mRNA expression was evaluated by real time PCR. Results: PRP-treated corneas exhibited reduced stromal cell apoptosis at day 3 and day 5 (p = 0.038, and 50.001, respectively) relative to controls. Interleukin-1b mRNA expression, however, was unchanged in PRP-treated corneas relative to controls. Topical PRP treatment resulted in a higher proportion of aSMA-positive myofibroblasts recruited to the wound site relative to control corneas. PRP did not affect activation of SMAD2 but activation of SMAD3 was significantly reduced at day 1 (p = 0.001) and dramatically increased at day 5 (p = 0.032). Conclusions: PRP treatment resulted in suppressed stromal cell apoptosis followed by SMAD3 activation and a greater proportion of myofibroblasts present at the wound site. Suppression of stromal cell apoptosis after corneal wounding by use of a growth factor-rich formulation may lead to myofibroblast accumulation by modulation of the TGF-b pathway.

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Section: Discussionmentioning

confidence: 99%

Platelet-Rich Plasma Prolongs Myofibroblast Accumulation in Corneal Stroma with Incisional Wound

Koulikovska

Szymanowski

Lagali

2015

Current Eye Research

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“…In the present study, for the preparation of PRP the method described by Gimeno et al (2006) was followed. The same method has been used in other experimental protocols in rabbits regarding ocular surface conditions (Gimeno et al, 2010;Tanidir et al, 2010;Khaksar et al, 2013). Intracardiac blood sampling was not performed, and blood was drawn from the jugular vein as it can be well tolerated by rabbits, no anaesthesia was required and the resulting blood collection was without clumps.…”

Section: Discussionmentioning

confidence: 99%

The effect of intrastromal PRP and oral doxycycline in corneal wound healing after alkali burn

Charalambidou

Kofidou

Papaioannou

et al. 2019

J Hellenic Vet Med Soc

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Ocular chemical burns are among the most critical and true ocular emergencies in dogs and cats, with destructive consequences on the ocular surface and intraocular tissues. Platelet alpha granules are an important reservoir of growth factors that can stimulate chemotaxis of inflammatory cells, mitosis, migration and differentiation of cells. On the other hand, tetracyclines and their derivatives exhibit non-antimicrobial properties, such as affecting inflammation, immunomodulation, cell proliferation and angiogenesis. The objective of this study is to investigate the effect of intrastromal platelet rich plasma (PRP) in combination with oral doxycycline in the healing process of the cornea. Thirty six New Zealand rabbits were used in this experimental study. Alkali burns were created by applying a round filter paper soaked in NaOH. PRP was prepared by centrifugation of autologous blood. The rabbits were allocated to four groups (9 animals each). Group 1 served as the control group without any treatment. Group 2 received doxycycline (5mg/kg b.w.) orally once a day throughout the study. Group 3 received an intrastromal injection of PRP (0.5ml) in the cornea, and group 4 received intrastromal PRP combined with oral doxycycline. Treatments had varied levels of success, with PRP combined with oral doxycycline producing the best results regarding corneal healing. Animals in the control group had epithelial defects for the whole duration of the study, and 3 animals presented corneal perforation. Groups 3 and 4 had smaller mean defect area, compared to groups 1 and 2. On the 7th day, neovascularisation was lower in treatment groups compared to the control group. Groups 3 and 4 also had less corneal oedema compared to the control group on day 3 and 7. Group 4 exhibited the best wound healing, with less neovascularization and better collagen arrangement, as shown by the histopathological evaluation. To the authors’ knowledge this is the first experimental study that intrastromal PRP is combined with oral doxycycline for the management of corneal chemical burns. This combination is a simple, safe and economical therapeutic approach that promotes corneal healing.

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“…Since autologous blood derivatives such as autologous serum (AS) and plasma rich in growth factors (PRGFs) have noticeable advantages such as availability, no risk of rejection and biocompatibility, they have attracted lots of attention [ 12 , 27 ]. Blood-derived proteins also contain various growth factors that promote tissue healing [ 43 ]. Besides blood derivatives, it has been shown that condition medium (CM) from various sources of stem cells contains an ample supply of growth factors that boost tissue regeneration [ 43 , 44 ].…”

Section: Overview Of Required Properties For Corneal Regenerationmentioning

confidence: 99%

“…Blood-derived proteins also contain various growth factors that promote tissue healing [ 43 ]. Besides blood derivatives, it has been shown that condition medium (CM) from various sources of stem cells contains an ample supply of growth factors that boost tissue regeneration [ 43 , 44 ]. List of various scaffold-free cell and growth factor sources used for treating corneal diseases are summarized in Table 1 .…”

Section: Overview Of Required Properties For Corneal Regenerationmentioning

confidence: 99%

Bioengineering Approaches for Corneal Regenerative Medicine

Mahdavi

Abdekhodaie

Mashayekhan

et al. 2020

Tissue Eng Regen Med

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BACKGROUND: Since the cornea is responsible for transmitting and focusing light into the eye, injury or pathology affecting any layer of the cornea can cause a detrimental effect on visual acuity. Aging is also a reason for corneal degeneration. Depending on the level of the injury, conservative therapies and donor tissue transplantation are the most common treatments for corneal diseases. Not only is there a lack of donor tissue and risk of infection/rejection, but the inherent ability of corneal cells and layers to regenerate has led to research in regenerative approaches and treatments. METHODS: In this review, we first discussed the anatomy of the cornea and the required properties for reconstructing layers of the cornea. Regenerative approaches are divided into two main categories; using direct cell/growth factor delivery or using scaffold-based cell delivery. It is expected delivered cells migrate and integrate into the host tissue and restore its structure and function to restore vision. Growth factor delivery also has shown promising results for corneal surface regeneration. Scaffold-based approaches are categorized based on the type of scaffold, since it has a significant impact on the efficiency of regeneration, into the hydrogel and non-hydrogel based scaffolds. Various types of cells, biomaterials, and techniques are well covered. RESULTS: The most important characteristics to be considered for biomaterials in corneal regeneration are suitable mechanical properties, biocompatibility, biodegradability, and transparency. Moreover, a curved shape structure and spatial arrangement of the fibrils have been shown to mimic the corneal extracellular matrix for cells and enhance cell differentiation. CONCLUSION: Tissue engineering and regenerative medicine approaches showed to have promising outcomes for corneal regeneration. However, besides proper mechanical and optical properties, other factors such as appropriate sterilization method, storage, shelf life and etc. should be taken into account in order to develop an engineered cornea for clinical trials.

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In vivo lamellar keratoplasty using platelet-rich plasma as a bioadhesive

Cited by 9 publications

References 25 publications

Platelet-Rich Plasma Prolongs Myofibroblast Accumulation in Corneal Stroma with Incisional Wound

Platelet-Rich Plasma Prolongs Myofibroblast Accumulation in Corneal Stroma with Incisional Wound

The effect of intrastromal PRP and oral doxycycline in corneal wound healing after alkali burn

Bioengineering Approaches for Corneal Regenerative Medicine

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