In Vivo Laser Confocal Microscopic Analysis of Murine Cornea and Lens Microstructures

Yuasa, Masashi; Kobayashi, Akira; Yokogawa, Hideaki; Sugiyama, Kazuhisa

doi:10.3928/15428877-20080901-20

Cited by 5 publications

(13 citation statements)

References 23 publications

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“…Many cellular and noncellular structures were notably larger in the horse than in other species, including individual cells of several layers of the corneal epithelium and nerve fibers. Possibly reflecting the greater thickness of the equine cornea or species‐specific anatomical variation, several structures were also readily imaged that have been previously reported as not visible in the normal cornea of other animal species when imaged with identical or similar confocal microscopes 11–19 . These structures included the basement membrane of the basal epithelium and corneal cells presumed to be Langerhans cells and corneal stromal dendritic cells.…”

Section: Discussionmentioning

confidence: 99%

“…In vivo confocal microscopy techniques have been applied to a variety of ocular structures, most notably the cornea 6–10 . Morphologic features of the normal cornea have been reported for several species, including birds, cats, dogs, humans, guinea pigs, mice, nonhuman primates, pigs, rabbits, and rats 11–19 . The objectives of the present study were to describe morphologic features, sublayer pachymetry, and endothelial cell density of the normal equine cornea by in vivo confocal microscopy.…”

Section: Introductionmentioning

confidence: 98%

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In vivoconfocal microscopy of the normal equine cornea and limbus

Ledbetter

Scarlett

2009

Veterinary Ophthalmology

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Objective To describe morphologic features, pachymetry and endothelial cell density of the normal equine cornea and limbus by in vivo confocal microscopy. Animals studied Ten horses without ocular disease. Procedure The central and peripheral corneas were examined with a modified Heidelberg Retina Tomograph II and Rostock Cornea Module using a combination of automated and manual image acquisition modes. Thickness measurements of various corneal layers were performed and endothelial cell density determined. Results Images of the constituent cellular and noncellular elements of the corneal epithelium, stroma, endothelium, and limbus were acquired in all horses. Corneal stromal nerves, the subepithelial nerve plexus, and the sub-basal nerve plexus were visualized. Cells with an appearance characteristic of Langerhans cells and corneal stromal dendritic cells were consistently detected in the corneal basal epithelium and anterior stroma, respectively. Median central total corneal thickness was 835 lm (range 725-920 lm) and median central corneal epithelial thickness was 131 lm Conclusions In vivo corneal confocal microscopy provides a noninvasive method of assessing normal equine corneal structure at the cellular level and is a precise technique for corneal sublayer pachymetry and cell density measurements. A resident population of presumed Langerhans cells and corneal stromal dendritic cells was detected in the normal equine cornea. The described techniques can be applied to diagnostic evaluation of corneal alternations associated with disease and have broad clinical and research applications in the horse.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

In vivoconfocal microscopy of the normal equine cornea and limbus

Ledbetter

Scarlett

2009

Veterinary Ophthalmology

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“…The literature has been controversial regarding whether there is an anterior limiting lamina and Descemet's membrane in the mouse cornea. 9,12,22,23 Our in vivo cornea findings clearly showed the anterior limiting lamina. This result is consistent with some of the previous findings.…”

Section: Discussionmentioning

confidence: 69%

“…In addition, the photographs of the cornea have usually shown cross-sectional views (perpendicular to the corneal surface) of the five full corneal layers in fixed corneal samples in vitro, 7,21 although images obtained with the in vivo confocal microscope generally consist of optical sections oriented parallel to the surface of the cornea. Representations of the different corneal cells without visible cell nuclei 9,12,22 did not show the ratio of the cell nuclei to the cytoplasm. Our study detailed all of the corneal cells with clear cell nuclei and cell boundaries from sections parallel to the surface of the cornea.…”

Section: Discussionmentioning

confidence: 92%

Two-Photon Imaging of the Cornea Visualized in the Living Mouse Using Vital Dyes

Zhang¹,

Wang²,

Liu³

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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“…using the sequence scanning mode, which allowed images to be repeatedly acquired at different focus positions, enabling the construction of a three-dimensional image. [9][10][11] The scanned images were recorded for analysis.…”

Section: Detection Of Corneal Foreign Bodies In Micementioning

confidence: 99%

The role of in vivo confocal microscopy in the diagnosis of hidden corneal foreign bodies

et al. 2013

J Int Med Res

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Objective: To investigate in vivo confocal microscopy (IVCM) to diagnose hidden corneal foreign bodies. Methods: Male Kunming mice (n ¼ 25; 12 weeks old) were anaesthetized prior to the insertion of five different materials (spiny wood, rusty iron, sharp stone, sharp glass fragment and human hair fragment) into the cornea by different traumatic processes. A separate mouse was used for each corneal foreign body. The corneas of the mice were scanned 24 h later by a laser scanning IVCM to establish the characteristics (shape, reflectivity and depth in the cornea) of each foreign body. These findings were used to help screen and identify corneal foreign bodies in patients. Corneal smears and scraping cultures were performed in cases of probable corneal infection. Results: Animal models for the five different foreign particles were established successfully, with each showing distinctive characteristics. These animal results were used to diagnose 41 patients with suspected corneal foreign bodies who were negative by slit lamp examination, but positive by IVCM (observational case series). The most prevalent type of hidden foreign body was plant material (51.2%), followed by metal (29.3%). Ten patients with corneal foreign bodies developed fungal keratitis, found using IVCM. Conclusions: Laser IVCM is an effective and reliable tool for the diagnosis of hidden corneal foreign bodies.

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In Vivo Laser Confocal Microscopic Analysis of Murine Cornea and Lens Microstructures

Abstract: In vivo laser confocal microscopy can provide high-resolution images of all corneal layers and lens structures of mice without sacrificing animals or tissue preparation.

Cited by 5 publications

References 23 publications

In vivoconfocal microscopy of the normal equine cornea and limbus

In vivoconfocal microscopy of the normal equine cornea and limbus

Two-Photon Imaging of the Cornea Visualized in the Living Mouse Using Vital Dyes

The role of in vivo confocal microscopy in the diagnosis of hidden corneal foreign bodies

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