In vivo liberation of silver ions from metallic silver surfaces

Danscher, Gorm; Locht, Linda J.

doi:10.1007/s00418-009-0670-5

Cited by 22 publications

(36 citation statements)

References 42 publications

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“…The kidney and liver, in particular have been described as central organs for the metabolism of silver in rats. 46,47 Our results indicate that at both AgNP concentrations of 0.2 and 0.4 µM, the silver concentration in the liver decreased after 72 h. Similarly, the metal concentration measured in the kidney decreased to levels close to those observed for the control group, suggesting that the silver was most likely excreted and not accumulated within that organ. Interestingly, silver accumulation in the lymph nodes for the 0.4 µM AgNPs was considerably higher than that measured for 0.2 µM.…”

Section: Subcutaneous Implants In Mouse Modelsupporting

confidence: 58%

“…These observations are in line with the proposed in the literature that indicates that the toxic effects, and accumulation, of silver in living organisms are directly connected with the formation of complexes between ionic silver and key enzymes. 46 Such toxicity was expected not be observed for silver nanocrystals bound to collagen, and other extracellular matrix proteins, 46 as the case of the AgNPs herein prepared.…”

Section: Subcutaneous Implants In Mouse Modelmentioning

confidence: 82%

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Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds

et al. 2015

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The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering.The resulting hybrid materials at [AgNPs] < 0.4 µM retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels; they also displayed remarkable anti-infective properties against S. aureus, S. epidermidis, E. coli and P. aeruginosa at considerably lower concentrations than silver nitrate. Further, subcutaneous implants of materials containing 0.2 µM AgNPs in mice showed a reduction in the levels of IL-6 and other inflammation markers (CCL24, sTNFR-2, and TIMP1). Finally, an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant. IntroductionBiomaterial-associated infections are a significant healthcare problem and have been linked to medical morbidity and death. [2][3][4][5][6] This has motivated the development of materials with anti-infective properties, such as biomaterials loaded with antibiotics. 7 However, with the increasing number of bacteria that are resistant to antibiotics, 8 silver with historically documented anti-microbial activity has re-gained its attractiveness as an alternative to antibiotics. 9 While toxic to bacteria, it unfortunately is also toxic to mammalian cells. 9,10 More recently, therefore, silver nanoparticles (AgNPs) have been evaluated as a safer alternative to ionic silver. 1,[11][12][13][14][15][16][17][18][19][20] The recent work from our team showed that in comparison with silver, biomolecule-coated, photochemically-produced AgNPs can have both bactericidal and bacteriostatic properties with almost negligible cytotoxic effects. 12 We also showed that oxidation of Ag to AgO is most likely the cause of the cytotoxic effects observed with AgNPs. Our overarching goal is to expand the safe use of AgNPs in the development of implantable hybrid-biomaterials with antiinfective properties for future use as scaffolds to enable regeneration of tissue and organs at a risk of bacterial colonization and concomitant biofilm formation like diabetic foot ulcers. Although some collagen-based materials including † Electronic supplementary information (ESI) available: Representative absorption spectra of AgNP@collagen nanoparticles before and after lyophilization. Absorption spectra for the washes obtained from a 1.0 µM AgNP hydrogel over the course of 5 days. Area under the curve (AUC) calculated from the absorption spectra of 500 µm thickness collagen hydrogels prepared using different concentrations of AgNP@collagen. Selected Cryo-SEM images of BDDGE type I collagen-based hydrogels in the absence or presence of 1.0 µM AgNP. An image of a sel...

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Section: Subcutaneous Implants In Mouse Modelsupporting

confidence: 58%

Section: Subcutaneous Implants In Mouse Modelmentioning

confidence: 82%

Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds

et al. 2015

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“…The fact that smaller and irregular nanoparticles show the highest antibiotic activity indicates that the surface area of the particles is important. The rate at which Ag + can be released is dependent on the surface area, and can thus be the determining factor for antibiotic activity [117,118]. The formation of a surface layer of oxidized Ag during production and storage might occur, and this may be a reservoir for the antimicrobial ions.…”

Section: Mechanism Of Antibacterial Action Of Silvermentioning

confidence: 99%

New Strategies in the Development of Antimicrobial Coatings: The Example of Increasing Usage of Silver and Silver Nanoparticles

2011

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Bacterial infection from medical devices is a major problem and accounts for an increasing number of deaths as well as high medical costs. Many different strategies have been developed to decrease the incidence of medical device related infection. One way to prevent infection is by modifying the surface of the devices in such a way that no bacterial adhesion can occur. This requires modification of the complete surface with, mostly, hydrophilic polymeric surface coatings. These materials are designed to be non-fouling, meaning that protein adsorption and subsequent microbial adhesion are minimized. Incorporation of antimicrobial agents in the bulk material or as a surface coating has been considered a viable alternative for systemic application of antibiotics. However, the manifestation of more and more multi-drug resistant bacterial strains restrains the use of antibiotics in a preventive strategy. The application of silver nanoparticles on the surface of medical devices has been used to prevent bacterial adhesion and subsequent biofilm formation. The nanoparticles are either deposited directly on the device surface, or applied in a polymeric surface coating. The silver is slowly released from the surface, thereby killing the bacteria present near the surface. In the last decade there has been a surplus of studies applying the concept of silver nanoparticles as an antimicrobial agent on a range of different medical devices. The main problem however is that the exact antimicrobial mechanism of silver remains unclear. Additionally, the antimicrobial efficacy of silver on OPEN ACCESSPolymers 2011, 3 341 medical devices varies to a great extent. Here we will review existing antimicrobial coating strategies and discuss the use of silver or silver nanoparticles on surfaces that are designed to prevent medical device related infections.

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“…Besides systemic administration of antibiofilm molecules to cure biofilmassociated infections, such molecules can be used as a coating on medical devices, thereby preventing proper biofilm formation of microbial pathogens on the device and thus resulting in a reduced risk for the development of biofilm-associated device infections. Current antibiofilm coatings of medical devices are mainly based on the use of silver ions, which are toxic upon accumulation (14), or on the release of standard antibiotics/antimycotics for which biofilms display increased resistance.…”

mentioning

confidence: 99%

Plant-Derived Decapeptide OSIP108 Interferes with Candida albicans Biofilm Formation without Affecting Cell Viability

Delattin

Brucker

Craik

et al. 2014

Antimicrob Agents Chemother

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We previously identified a decapeptide from the model plant Arabidopsis thaliana, OSIP108, which is induced upon fungal pathogen infection. In this study, we demonstrated that OSIP108 interferes with biofilm formation of the fungal pathogen Candida albicans without affecting the viability or growth of C. albicans cells. OSIP108 displayed no cytotoxicity against various human cell lines. Furthermore, OSIP108 enhanced the activity of the antifungal agents amphotericin B and caspofungin in vitro and in vivo in a Caenorhabditis elegans-C. albicans biofilm infection model. These data point to the potential use of OSIP108 in combination therapy with conventional antifungal agents. In a first attempt to unravel its mode of action, we screened a library of 137 homozygous C. albicans mutants, affected in genes encoding cell wall proteins or transcription factors important for biofilm formation, for altered OSIP108 sensitivity. We identified 9 OSIP108-tolerant C. albicans mutants that were defective in either components important for cell wall integrity or the yeast-to-hypha transition. In line with these findings, we demonstrated that OSIP108 activates the C. albicans cell wall integrity pathway and that its antibiofilm activity can be blocked by compounds inhibiting the yeast-to-hypha transition. Furthermore, we found that OSIP108 is predominantly localized at the C. albicans cell surface. These data point to interference of OSIP108 with cell wall-related processes of C. albicans, resulting in impaired biofilm formation.

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In vivo liberation of silver ions from metallic silver surfaces

Cited by 22 publications

References 42 publications

Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds

Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds

New Strategies in the Development of Antimicrobial Coatings: The Example of Increasing Usage of Silver and Silver Nanoparticles

Plant-Derived Decapeptide OSIP108 Interferes with Candida albicans Biofilm Formation without Affecting Cell Viability

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