In vivo lymphatic targeting of methylene blue with microemulsion and multiple microemulsion

Wang, Shujun; Yang, Rui; Yao, Huimin; Zhou, Guoqin; Zhang, Yinghui; Yang, Boyang; Ng, Lawrence; Yan, Macheng

doi:10.1080/10717540903075644

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…It also provides protection against enzymatic hydrolysis (Hu et al, 2011). The selection of suitable surfactant combinations is a critical parameter in order to make thermodynamically stable emulsion (Wang et al, 2009). Establishment of a phase diagram is also necessary for the selection of multiple components in microemulsions (Narang et al, 2007).…”

Section: Microemulsionmentioning

confidence: 99%

Advances in nanotechnology-based carrier systems for targeted delivery of bioactive drug molecules with special emphasis on immunotherapy in drug resistant tuberculosis – a critical review

et al. 2015

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From the early sixteenth and seventeenth centuries to the present day of life, tuberculosis (TB) still is a global health threat with some new emergence of resistance. This type of emergence poses a vital challenge to control TB cases across the world. Mortality and morbidity rates are high due to this new face of TB. The newer nanotechnology-based drug-delivery approaches involving micro-metric and nano-metric carriers are much needed at this stage. These delivery systems would provide more advantages over conventional systems of treatment by producing enhanced therapeutic efficacy, uniform distribution of drug molecule to the target site, sustained and controlled release of drug molecules and lesser side effects. The main aim to develop these novel drug-delivery systems is to improve the patient compliance and reduce therapy time. This article reviews and elaborates the new concepts and drug-delivery approaches for the treatment of TB involving solid-lipid particulate drug-delivery systems (solid-lipid micro- and nanoparticles, nanostructured lipid carriers), vesicular drug-delivery systems (liposomes, niosomes and liposphere), emulsion-based drug-delivery systems (micro and nanoemulsion) and some other novel drug-delivery systems for the effective treatment of tuberculosis and role of immunomodulators as an adjuvant therapy for management of MDR-TB and XDR-TB.

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Section: Microemulsionmentioning

confidence: 99%

Advances in nanotechnology-based carrier systems for targeted delivery of bioactive drug molecules with special emphasis on immunotherapy in drug resistant tuberculosis – a critical review

et al. 2015

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“…Although there are different methods for quantifying MB and MET in their isolated form or associated with other substances (Chengalva, Angala Parameswari, & Aruna, 2016;Chengnan et al, 2019;Chhetri, Thapa, & Van Schepdael, 2014;Khdair, Handa, Mao, & Panyam, 2009;Prasanna & Priyanka, 2015;Rengelshausen et al, 2004;Rostamkalaei, Akbari, Saeedi, Morteza-Semnani, & Nokhodchi, 2019;Umapathi, Ayyappan, & Darlin Quine, 2012;Wang et al, 2009), few studies quantify these substances in a matrix as complex as the skin (Chen et al, 2020;Garcia et al, 2018;Junqueira et al, 2016;Polonini et al, 2019;Yu et al, 2018Yu et al, , 2019 and some of them employ quantitative methods that are not selective (Garcia et al, 2018;Junqueira et al, 2016) or standardized for other matrices such as plasma and urine, which may not be suitable for quantification in the different layers of the skin (Yu et al, 2018(Yu et al, , 2019; moreover, there are no studies in the literature that quantify the two substances simultaneously and in the different layers of the skin (SC and viable epidermis + dermis, VE + D).…”

Section: Introductionmentioning

confidence: 99%

Developing an analytical method by HPLC for simultaneous quantification of methylene blue and metformin applied to in vitro skin permeation and retention studies

Matos

Calixto

Garcı́a

2021

Biomedical Chromatography

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The aim of this study was to develop an HPLC method for simultaneous quantification of metformin (MET) and methylene blue (MB) in in vitro skin permeation/retention studies, in which retention was evaluated in the different layers of the skin [stratum corneum (SC) and the viable epidermis + dermis (VE + D)]. The method was validated considering the following parameters: specificity, linearity, quantitation limit (LOQ), recovery, precision and accuracy. Calibration curves were obtained using the following six matrices: methanol, water, methanolic extracts from the SC and VE + D spiked with the drugs and drugs extracted from the SC and VE + D. The precision, accuracy and LOQ of the method were evaluated in water and in VE + D and SC, applying the drug extraction process. The results show that the method is selective and linear for both drugs. The precision and accuracy values, independent of matrix and drug, were below the limit of 15%. The LOQ of MB was defined as 0.4 μg/ml in the VE + D and SC and 0.8 μg/ml in water. The LOQ of MET was defined as 0.8 μg/ml in the VE + D and SC and 0.4 μg/ml in the water. The recovery of the method was adequate, consistent and reproducible for the concentration range of 0.4-10 μg/ml for MB (73.3-92.1%) and 0.8-10.0 μg/mL for . This method has a potential application in the development of formulation for skin delivery of MB and MET.

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“…Consequently, MB-S could only maintain the bluestained state in LNs for a short period. For MB-ME, with the viscosity of 102.75 mPa S (Wang et al, 2009), it exhibited a poor fluidity, leading to a slow removal in lymph. Thus, T max , C max and AUC (0-12h) value of MB-ME in LNs were smaller than other formulations.…”

Section: Lymphatic Uptake and Retentionmentioning

confidence: 99%

“…MB-ME and MB-MME prepared in our previous study (Wang et al, 2009) demonstrated that microemulsion could enhance lymphatic uptake and drainage. However, due to the insoluble property of AB in majority of the solvents, it is difficult to construct nanoparticulate with high drug payload.…”

Section: Study On Drug Concentration In Plasmamentioning

confidence: 99%

“…Separately, PAMAM-AB with the concentration of 2 mg/kg, 5 mg/kg and 10 mg/kg (equivalent to the concentration of AB residues in PAMAM-AB), MB-S, MB-ME and MB-MME with the concentration of 10 mg/kg of body weight were injected subcutaneously into the footpad of the right hindpaw. MB-S, MB-ME and MB-MME were prepared according to our previous study (Wang et al, 2009). After 10 min of administration, the mice were sacrificed.…”

Section: Lymphatic Tracing Studymentioning

confidence: 99%

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Synthesis of a novel polyamidoamine dendrimer conjugating with alkali blue as a lymphatic tracer and study on the lymphatic targeting in vivo

Yang

Xia

et al. 2014

Drug Delivery

Self Cite

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In this study, a novel lymphatic tracer polyamidoamin-alkali blue (PAMAM-AB) was synthesized in order to evaluate the intra-lymphatic targeting ability and lymphatic tropism of PAMAM-AB after subcutaneous administration. UV-Vis, FT-IR, NMR and HPLC characterization were performed to prove the successful synthesis of PAMAM-AB. The calculated AB payload of PAMAM-AB conjugate was seven per dendrimer molecule (27.16% by weight). Hydrolysis stability of PAMAM-AB in vitro was evaluated, which was stable in PBS and human plasma. Lymphatic tracing were studied to determine the blue-stained intensity of PAMAM-AB in right popliteral lymph nodes (PLNs), iliac lymph nodes (ILNs) and para-aortic lymph nodes (PALNs) after subcutaneous administration. The pharmacokinetics and biodistribution of PAMAM-AB in mice were investigated. PLNs, ILNs and PALNs could be obviously blue-stained within 10 min after PAMAM-AB administration, and displayed a more rapid lymphatic absorption, a higher AUC value in lymph nodes and a longer lymph nodes residence time compared with methylene blue solution (MB-S), MB water-in-oil microemulsion (MB-ME), MB multiple microemulsion (MB-MME). Enhanced lymphatic drainage from the injection site and uptake into lymph of PAMAM-AB indicated that PAMAM-AB possesses the double function of lymphatic tracing and lymphatic targeting, and suggested the potential for the development of lymphatic targeting vectors or as a lymphatic tracer in its own right.

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In vivo lymphatic targeting of methylene blue with microemulsion and multiple microemulsion

Abstract: (2009) In vivo lymphatic targeting of methylene blue with microemulsion and multiple microemulsion, Drug Delivery, 16:7,[371][372][373][374][375][376][377]

Cited by 7 publications

References 23 publications

Advances in nanotechnology-based carrier systems for targeted delivery of bioactive drug molecules with special emphasis on immunotherapy in drug resistant tuberculosis – a critical review

Advances in nanotechnology-based carrier systems for targeted delivery of bioactive drug molecules with special emphasis on immunotherapy in drug resistant tuberculosis – a critical review

Developing an analytical method by HPLC for simultaneous quantification of methylene blue and metformin applied to in vitro skin permeation and retention studies

Synthesis of a novel polyamidoamine dendrimer conjugating with alkali blue as a lymphatic tracer and study on the lymphatic targeting in vivo

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