In vivo microscopy of the subepithelial capillary plexus of the endometrium of rats during embryo implantation

Tawia, S.A.; Rogers, Peter A. W.

doi:10.1530/jrf.0.0960673

Cited by 20 publications

(10 citation statements)

References 14 publications

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“…A possible role for the high levels of VEGF expression seen at the site of implantation is suggested by recent measurements of capillary diameter at the implantation site in rats (Tawia and Rogers, 1992). These authors showed that large increases in capillary diameter occur in response to local signals associated with the implanting embryo, and suggest that these are associ¬ ated with alterations in the endothelial cells or the basement membrane.…”

Section: Discussionmentioning

confidence: 99%

Expression of mRNA for vascular endothelial growth factor in human placenta

Sharkey

Charnock-Jones²,

Boocock³

et al. 1993

Reproduction

219

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Implantation and growth of the placenta requires extensive angiogenesis to establish the vascular structures involved in exchange. Failure to establish adequate blood supply to the fetus may have serious clinical consequences such as intrauterine growth retardation. Vascular endothelial cell growth factor (VEGF) is a recently identified growth factor with significant angiogenic properties. We have demonstrated the presence of four species of mRNA encoding VEGF in both first trimester and term placenta. In situ hybridization was used to localize the sites of expression of VEGF mRNA in these tissues. VEGF expression was seen in villous trophoblast in the first trimester and in extravillous trophoblast at term, and in both fetal macrophages within the villi and maternal macrophages in the decidua. Glandular epithelium in maternal decidua also expressed VEGF mRNA. The strongest site of expression was in maternal macrophages adjacent to Nitabuch's stria, a zone of necrosis at the site of implantation. This complex pattern of expression suggests that VEGF is involved in angiogenesis on both maternal and fetal sides of the placenta and that macrophages are the primary source of VEGF. However, VEGF may also play a role in term placenta, when extensive angiogenesis has diminished, possibly regulating vascular permeability.

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Section: Discussionmentioning

confidence: 99%

Expression of mRNA for vascular endothelial growth factor in human placenta

Sharkey

Charnock-Jones²,

Boocock³

et al. 1993

Reproduction

219

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“…This difference may also have been due to the effects of fixation artefact and shrinkage in histology specimens as well as a consequence of studying perfused vessels in live human subjects versus non-perfused vessels in post-mortem eyes (Rastogi et al, 2013). Other plausible explanations for increased capillary diameter in svOCT images may be due to the dynamic effects of physiological variables such as blood pressure, tissue interstitial pressure and vessel wall elasticity acting upon capillary beds (Tawia and Rogers, 1992). These variables have been shown to influence the anatomical dimensions of live tissue.…”

Section: Discussionmentioning

confidence: 99%

In vivo optical imaging of human retinal capillary networks using speckle variance optical coherence tomography with quantitative clinico-histological correlation

Chan

Balaratnasingam

et al. 2015

Microvascular Research

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Retinal capillary networks are critically linked to neuronal health and disease. The ability to perform accurate in vivo examination of human retinal capillary networks is therefore valuable for studying mechanisms that govern retinal homeostasis and retinal vascular diseases. Speckle variance optical coherence tomography (svOCT) is a non-invasive imaging technique that has the capacity to provide angiographic information about the retinal circulation. The application of this technology for studying human retinal capillary networks however has not been validated in a quantifiable manner. We use a custom-built svOCT device to qualitatively and quantitatively study the various capillary networks in the human perifovea. Capillary networks corresponding to the nerve fibre layer (NFL), the retinal ganglion cell/superficial inner plexiform layer (RGC/sIPL), the deep inner plexiform layer/superficial inner nuclear layer (dIPL/sINL) and the deep inner nuclear layer (dINL) are imaged in 9 normal human subjects. Measurements of capillary diameter and capillary density are made from each of these networks and results are compared to post-mortem histological data acquired with confocal scanning laser microscopy. Additionally, retinal capillary measurements from high-resolution fundus fluorescein angiogram (FA) are directly compared with svOCT images from 6 eyes. We demonstrate that svOCT images of capillary networks are morphologically comparable to microscopic images of histological specimens. Similar to histological images in svOCT images, the capillaries in the NFL network run parallel to the direction of RGC axons while capillaries in the dINL network comprise a planar configuration with multiple closed loops. Capillaries in remaining networks are convoluted with a complex three-dimensional architecture. We demonstrate that there is no significant difference in capillary density measurements between svOCT and histology images for all networks. Capillary diameter was significantly greater in svOCT images compared to histology for all networks. Capillary density measurements were also higher in svOCT compared to FA. The results of this study suggest that in vivo svOCT imaging allows accurate morphometric assessment of capillary networks in the human perifovea and may provide an improved ability to render microvascular detail compared to FA. Therefore, svOCT may have broad clinical applications in the study of human retinal physiology and disease. The difference in quantitative measurements between svOCT and histology may reflect dynamic variations in the retinal microcirculation and warrants further investigation.

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“…Despite the significant impact on future generations, our understanding of the uterine microcirculation in either pregnant (Rogers, 1992; Tawia and Rogers, 1992; Ruttner et al, 2000) or non-pregnant states (Rogers and Macpherson, 1990; Alsip et al, 1996; Hill et al, 1997; Sweeney et al, 1999) remains limited (Osol and Moore, 2014). While isolated microvascular preparations have provided crucial mechanistic data regarding vascular remodeling associated with pregnancy and gestational disease (e.g.…”

Section: Discussionmentioning

confidence: 99%

Uterine microvascular sensitivity to nanomaterial inhalation: An in vivo assessment

Stapleton

McBride

et al. 2015

Toxicology and Applied Pharmacology

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With the tremendous number and diverse applications of engineered nanomaterials incorporated in daily human activity, exposure can no longer be solely confined to occupational exposures of healthy male models. Cardiovascular and endothelial cell dysfunction have been established using in vitro and in situ preparations, but the translation to intact in vivo models is limited. Intravital microscopy has been used extensively to understand microvascular physiology while maintaining in vivo neurogenic, humoral, and myogenic control. However, a tissue specific model to assess the influences of nanomaterial exposure on female reproductive health has not been fully elucidated. Female Sprague Dawley (SD) rats were exposed to nano-TiO2 aerosols (171 ± 6 nm, 10.1 ± 0.39 mg/m3, 5 h) 24-hours prior to experimentation, leading to a calculated deposition of 42.0 ± 1.65 μg. After verifying estrus status, vital signs were monitored and the right horn of the uterus was exteriorized, gently secured over an optical pedestal, and enclosed in a warmed tissue bath using intravital microscopy techniques. After equilibration, significantly higher leukocyte-endothelium interactions were recorded in the exposed group. Arteriolar responsiveness was assessed using ionophoretically applied agents: muscarinic agonist acetylcholine (0.025 M; ACh; 20, 40, 100, and 200 nA), and nitric oxide donor sodium nitroprusside (0.05 M; SNP; 20, 40, and 100 nA), or adrenergic agonist phenylephrine (0.05 M; PE; 20, 40, and 100 nA) using glass micropipettes. Passive diameter was established by tissue superfusion with 10−4 M adenosine. Similar to male counterparts, female SD rats present systemic microvascular dysfunction; however the ramifications associated with female health and reproduction have yet to be elucidated.

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In vivo microscopy of the subepithelial capillary plexus of the endometrium of rats during embryo implantation

Cited by 20 publications

References 14 publications

Expression of mRNA for vascular endothelial growth factor in human placenta

Expression of mRNA for vascular endothelial growth factor in human placenta

In vivo optical imaging of human retinal capillary networks using speckle variance optical coherence tomography with quantitative clinico-histological correlation

Uterine microvascular sensitivity to nanomaterial inhalation: An in vivo assessment

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