In Vivo Molecular Imaging Analysis of a Nasal Vaccine That Induces Protective Immunity against Botulism in Nonhuman Primates

Yuki, Yoshikazu; Nochi, Tomonori; Harada, Norihiro; Katakai, Yuko; Shibata, Hiroaki; Mejima, Mio; Kohda, Tomoko; Tokuhara, Daisuke; Kurokawa, Shiho; Takahashi, Yuko; Ono, Fumiko; Kozaki, Shunji; Terao, Keiji; Tsukada, Hideo; Kiyono, Hiroshi

doi:10.4049/jimmunol.1001789

Cited by 30 publications

(20 citation statements)

References 29 publications

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“…We assigned the subject animals to the following age groups: Growth group, ≤6 years (n=9); Adult group, 7–25 years (n=43); and Aged group, >26 years (n=10). These age divisions were determined based on previous research using macaques [4, 20, 29, 30]. Additionally, the Aged group was modified to account for the lifestyle of captive cynomolgus monkeys.…”

Section: Methodsmentioning

confidence: 99%

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Establishment of reference values for complete blood count and blood gases in cynomolgus monkeys (<i>Macaca fascicularis</i>)

Nakayama

Kanayama

Katakai

et al. 2017

The Journal of Veterinary Medical Science

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Cynomolgus monkeys are closely related to humans phylogenetically, and this has resulted in their widespread use as a preclinical model. Hematological data with regard to these monkeys are thus important. Although reference values for blood components and sex hormones have been established for cynomolgus monkeys, those for arterial blood gases have not. The arterial blood gases quickly reflect respiratory and circulatory dynamics, and are thus useful for animal management and safe general anesthesia and surgical operations. Furthermore, since O2 is transported by RBC, CBC and blood gases are closely related. The present study aimed to establish reference values for arterial blood gases and CBC in cynomolgus monkeys over a wide age range. Blood gases and CBC of arterial blood, collected from 41 female and 21 male anesthetized monkeys, were measured. Age correlated with RBC, HGB and HCT in the CBC. Values differed significantly between males and females in pCO2, CO2 concentration, MCV and MCH. The pH of blood was equivalent to that of humans and pCO2 was more stable, whereas MCV and MCH were lower than those in humans. Erythrocytes were smaller and less pigmented than in other Macaca species. Several relationships between gender and age, and blood gases and CBC were identified in cynomolgus monkeys. In conclusion, these reference values will be useful as markers for veterinary applications and in the care and maintenance of these animals.

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Section: Methodsmentioning

confidence: 99%

“…These macaques are used for numerous research projects at this institution, including vaccine, infectious disease, gene therapy, regenerative medicine and aging studies. This colony is the largest primate experimental colony in Japan, and is used by researchers throughout the world for advanced clinical studies [2, 29, 30]. …”

mentioning

confidence: 99%

Establishment of reference values for complete blood count and blood gases in cynomolgus monkeys (<i>Macaca fascicularis</i>)

Nakayama

Kanayama

Katakai

et al. 2017

The Journal of Veterinary Medical Science

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“…Tagging DC in vivo through the use of labeled antigen is a convenient trick [17, 24]. For example, a nasal vaccine consisting of 18 F-labeled botulinum neurotoxin was imaged in real time and in a quantitative manner using PET in primates [159]. In this study, the investigators demonstrated that nasal administration is safe with respect to spreading antigens to the central nervous system.…”

Section: Immunological Targets For Imaging In Humansmentioning

confidence: 97%

In vivo imaging of therapy-induced anti-cancer immune responses in humans

Aarntzen

Srinivas

Radu³

et al. 2012

Cell. Mol. Life Sci.

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Immunotherapy aims to re-engage and revitalize the immune system in the fight against cancer. Research over the past decades has shown that the relationship between the immune system and human cancer is complex, highly dynamic, and variable between individuals. Considering the complexity, enormous effort and costs involved in optimizing immunotherapeutic approaches, clinically applicable tools to monitor therapy-induced immune responses in vivo are most warranted. However, the development of such tools is complicated by the fact that a developing immune response encompasses several body compartments, e.g., peripheral tissues, lymph nodes, lymphatic and vascular systems, as well as the tumor site itself. Moreover, the cells that comprise the immune system are not static but constantly circulate through the vascular and lymphatic system. Molecular imaging is considered the favorite candidate to fulfill this task. The progress in imaging technologies and modalities has provided a versatile toolbox to address these issues. This review focuses on the detection of therapy-induced anticancer immune responses in vivo and provides a comprehensive overview of clinically available imaging techniques as well as perspectives on future developments. In the discussion, we will focus on issues that specifically relate to imaging of the immune system and we will discuss the strengths and limitations of the current clinical imaging techniques. The last section provides future directions that we envision to be crucial for further development.

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“…However, insufficient access to adequate structures and equipment for imaging NHPs associated with suitable confinement for class 2 and 3 pathogens, in large part explains the relatively limited exploration of pathogen transmission and dissemination using in vivo imaging in NHPs. Nevertheless, in vivo imaging is entirely feasible in larger animals such as monkeys [68,69]; even though adult male Aotus monkeys and rhesus macaques weigh about 1 and 8 kg on average, respectively, and are about 35 and 55 cm long, respectively [70] (mice weigh 20-30 g and are 6 cm long).…”

Section: Simian Plasmodium Species In Macaquesmentioning

confidence: 99%

“…The immune response to viral infection in SHIV-infected macaques has also been explored by SPECT [99] and delivery of a botulism vaccine has been studied by a combination of PET and MRI [69]. Also, [ 18 F]FDG PET/CT imaging has also been used to assess novel combinations of new drugs in NHP models of tuberculosis [100].…”

Section: In Vivo Imaging and Infectious Diseases In Nhpsmentioning

confidence: 99%

In vivo imaging in NHP models of malaria: Challenges, progress and outlooks

Beignon

Grand

Chapon

2014

Parasitology International

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Animal models of malaria, mainly mice, have made a large contribution to our knowledge of host-pathogen interactions and immune responses, and to drug and vaccine design. Non-human primate (NHP) models for malaria are admittedly under-used, although they are probably closer models than mice for human malaria; in particular, NHP models allow the use of human pathogens (Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium knowlesi). NHPs, whether natural hosts or experimentally challenged with a simian Plasmodium, can also serve as robust pre-clinical models. Some simian parasites are closely related to a human counterpart, with which they may share a common ancestor, and display similar major features with the human infection and pathology. NHP models allow longitudinal studies, from the early events following sporozoite inoculation to the later events, including analysis of organs and tissues, particularly liver, spleen, brain and bone marrow. NHP models have one other significant advantage over mouse models: NHPs are our closest relatives and thus their biology is very similar to ours. Recently developed in vivo imaging tools have provided insight into malaria parasite infection and disease in mouse models. One advantage of these tools is that they limit the need for invasive procedures, such as tissue biopsies. Many such technologies are now available for NHP studies and provide new opportunities for elucidating host/parasite interactions. The aim of this review is to bring the malaria community up to date on what is currently possible and what soon will be, in terms of in vivo imaging in NHP models of malaria, to consider the pros and the cons of the various techniques, and to identify challenges.

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In Vivo Molecular Imaging Analysis of a Nasal Vaccine That Induces Protective Immunity against Botulism in Nonhuman Primates

Abstract: Material

Cited by 30 publications

References 29 publications

Establishment of reference values for complete blood count and blood gases in cynomolgus monkeys (<i>Macaca fascicularis</i>)

Establishment of reference values for complete blood count and blood gases in cynomolgus monkeys (<i>Macaca fascicularis</i>)

In vivo imaging of therapy-induced anti-cancer immune responses in humans

In vivo imaging in NHP models of malaria: Challenges, progress and outlooks

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