In vivo monitoring of protein-bound and free NADH during ischemia by nonlinear spectral imaging microscopy

Palero, Jonathan A.; Bader, Arjen N.; Bruijn, Henriëtte S. de; Heuvel, Angélique van der Ploeg van den; Sterenborg, Henricus J. C. M.; Gerritsen, Hans C.

doi:10.1364/boe.2.001030

Cited by 57 publications

(51 citation statements)

References 31 publications

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“…In addition, similar AF intensity and spectral line shapes to that of in vivo tissue have been reported for freshly excised tissue in a hamster cheek pouch model [71]. In a study of ischaemic mouse skin by Palero et al [72] using spectrally resolved multiphoton microscopy, the change in fluorescence intensity was reported to increase by 71% compared to baseline over approximately one hour and the change in peak emission wavelength shifted from 456 nm to 450 nm after 20 minutes. It is clear that caution should be exercised when trying to extrapolate ex vivo findings to what could be expected in vivo.…”

Section: Discussionsupporting

confidence: 59%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

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Abstract:We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime −570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens. References and links1. R. Lambert, H. Saito, and Y. Saito, "High-resolution endoscopy and early gastrointestinal cancer...dawn in the East," Endoscopy 39(3), 232-237 (2007

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Section: Discussionsupporting

confidence: 59%

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Coda¹,

Thompson²,

Kennedy³

et al. 2014

Biomed. Opt. Express

View full text Add to dashboard Cite

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“…Autofluorescence emitting from tissues has many intrinsic fluorophores, such as reduced nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), and porphyrins [13,14]. NADH and FAD are metabolic coenzymes in oxidative phosphorylation, and NADH has a free or protein-bound state [15,16]. Porphyrins are organic compounds and are often used as a tumor-specific marker [17].…”

Section: Introductionmentioning

confidence: 99%

“…Bard et al used white-light reflectance spectroscopy during bronchoscopy examination to differentiate normal bronchus mucosa from malignant lesions [15,20]. Fawzy et al differentiated bronchial cancerous lesions with fluorescence spectroscopy, and reached a sensitivity and specificity of 71% and 74% [21].…”

Section: Introductionmentioning

confidence: 99%

Autofluorescence Imaging and Spectroscopy of Human Lung Cancer

et al. 2016

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Lung cancer is one of the most common cancers, with high mortality rate worldwide. Autofluorescence imaging and spectroscopy is a non-invasive, label-free, real-time technique for cancer detection. In this study, lung tissue sections excised from patients were detected by laser scan confocal microscopy and spectroscopy. The autofluorescence images demonstrated the cellular morphology and tissue structure, as well as the pathology of stained images. Based on the spectra study, it was found that the majority of the patients showed discriminating fluorescence in tumor tissues from normal tissues. Therefore, autofluorescence imaging and spectroscopy may be a potential method for aiding the diagnosis of lung cancer.

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“…[16][17][18] Speci¯cally, 1 represents the relative concentration of free NAD(P)H, and 2 and 3 represent the relative concentration of the protein-bound NAD(P)H. 16 Therefore, the decrease of the mean lifetime of NAD(P)H in leukemic myeloid cells observed in this study was attributed to the change of relative concentration of free and protein-bound NAD(P)H.…”

Section: Discussionmentioning

confidence: 58%

Characterizing Fluorescence Lifetime of Nad(p)h in Human Leukemic Myeloid Cells and Mononuclear Cells

Lin

Liu

Huang

et al. 2013

J. Innov. Opt. Health Sci.

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The aim of this ex vivo study was to explore the potential of using the°uorescence lifetime of intracellular reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) as a label-free indicator to characterize the di®erences between human leukemic myeloid cells and normal mononuclear cells (MNC). The steady-state and time-resolved auto°uorescence of two human leukemic myeloid cell lines (K562, HL60) and MNC were measured by a spectro°uorimeter. According to excitation-emission matrix (EEM) analysis, the optimal emission of NAD(P)H in these cells suspensions occurred at 445 nm. Furthermore, the°uorescence lifetimes of NAD(P)H in leukemic myeloid cells and MNC were determined by¯tting the time-resolved auto°uorescence data. The mean°uorescence lifetimes of NAD(P)H in K562, HL60, and MNC cells were 5:57AE 1:19, 4:45 AE 0:71, and 7:31 AE 0:60 ns, respectively. There was a signi¯cant di®erence in the mean lifetime of NAD(P)H between leukemic myeloid cells and MNC (p < 0:05). The di®erence was essentially caused by the change in relative concentration of free and protein-bound NAD(P)H. This study suggests that the mean°uorescence lifetime of NAD(P)H might be a potential labelfree indicator for di®erentiating leukemic myeloid cells from MNC.

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In vivo monitoring of protein-bound and free NADH during ischemia by nonlinear spectral imaging microscopy

Cited by 57 publications

References 31 publications

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Autofluorescence Imaging and Spectroscopy of Human Lung Cancer

Characterizing Fluorescence Lifetime of Nad(p)h in Human Leukemic Myeloid Cells and Mononuclear Cells

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