In vivo monitoring the dynamic process of acute retinal hemorrhage and repair in zebrafish with spectral‐domain optical coherence tomography

Lin, Yunting; Xiang, Xiang; Chen, Tingru; Mao, Guangjuan; Deng, Lijun; Zeng, Lvming; Zhang, Jian

doi:10.1002/jbio.201900235

Cited by 11 publications

(9 citation statements)

References 37 publications

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“…To our knowledge, several other studies use OCT to evaluate retinal damage in zebrafish. 26,30,[45][46][47][48] The study by Bailey et al 26 uses pan-retinal models of damage (retinal ablation with light and ouabain injection) and analyzes retinal changes from B-scans. Weber et al 30 uses both diffuse light and focused light approaches to ablate the retina.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive Imaging of Cone Ablation and Regeneration in Zebrafish

Huckenpahler

Lookfong

Warr

et al. 2020

Trans. Vis. Sci. Tech.

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To observe and characterize cone degeneration and regeneration in a selective metronidazole-mediated ablation model of ultraviolet-sensitive (UV) cones in zebrafish using in vivo optical coherence tomography (OCT) imaging. Methods: Twenty-six sws1:nfsB-mCherry;sws2:eGFP zebrafish were imaged with OCT, treated with metronidazole to selectively kill UV cones, and imaged at 1, 3, 7, 14, 28, or 56 days after ablation. Regions 200 × 200 μm were cropped from volume OCT scans to count individual UV cones before and after ablation. Fish eyes were fixed, and immunofluorescence staining was used to corroborate cone density measured from OCT and to track monocyte response. Results: Histology shows significant loss of UV cones after metronidazole treatment with a slight increase in observable blue cone density one day after treatment (Kruskal, Wallis, P = 0.0061) and no significant change in blue cones at all other timepoints. Regenerated UV cones measured from OCT show significantly lower density than pre-coneablation at 14, 28, and 56 days after ablation (analysis of variance, P < 0.01, P < 0.0001, P < 0.0001, respectively, 15.9% of expected nonablated levels). Histology shows significant changes to monocyte morphology (mixed-effects analysis, P < 0.0001) and retinal position (mixed-effects analysis, P < 0.0001). Conclusions: OCT can be used to observe loss of individual cones selectively ablated by metronidazole prodrug activation and to quantify UV cone loss and regeneration in zebrafish. OCT images also show transient changes to the blue cone mosaic and inner retinal layers that occur concomitantly with selective UV cone ablation. Translational Relevance: Profiling cone degeneration and regeneration using in vivo imaging enables experiments that may lead to a better understanding of cone regeneration in vertebrates.

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Section: Discussionmentioning

confidence: 99%

Noninvasive Imaging of Cone Ablation and Regeneration in Zebrafish

Huckenpahler

Lookfong

Warr

et al. 2020

Trans. Vis. Sci. Tech.

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“…The zebrafish has emerged as a very powerful tool for investigating the in vivo developmental toxicity of compounds on a large scale [33][34][35]. The small size, ease of genetic manipulations, and relatively economical cost has paved the way for zebrafish to be the best organism for human disease models [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53]. The zebrafish developmental toxicity study revealed the chronic toxicity of CM1 and CM2.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Evaluation and Anti-Angiogenic Effects of Two Furano-Sesquiterpenoids from Commiphora myrrh Resin

et al. 2020

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Commiphora myrrh resin (Myrrh) has been used in traditional Arabic medicine to treat various inflammatory diseases. Two furano-sesquiterpenoids, 2-methoxyfuranodiene (CM1) and 2-acetoxyfuranodiene (CM2), were isolated from the chloroform fraction of the ethanolic extract of Arabic Commiphora myrrh resin. The cytotoxicity of the compounds was evaluated using human liver carcinoma, breast cancer cells (HepG2 and MCF-7, respectively) and normal human umbilical vein endothelial cells (HUVECs) cell lines. The development toxicity and anti-angiogenic activity of both compounds were also evaluated using zebrafish embryos. Cell survival assays demonstrated that both compounds were highly cytotoxic in HepG2 and MCF7 cells, with IC50 values of 3.6 and 4.4 µM, respectively. Both compounds induced apoptosis and caused cell cycle arrest in treated HepG2 cells, which was observed using flow cytometric analysis. The development toxicity in zebrafish embryos showed the chronic toxicity of both compounds. The toxicity was only seen when the embryos remained exposed to the compounds for more than three days. The compound CM2 showed a significant level of anti-angiogenic activity in transgenic zebrafish embryos at sublethal doses. Thus, we demonstrated the cytotoxic properties of both compounds, suggesting that the molecular mechanism of these compounds should be further assessed.

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“…This paper adopted optical coherence tomography (OCT), a mature optical interference imaging technology widely applied in various fields, primarily the biomedical domain [25][26][27][28]. Structurally, it is similar to the Michelson interferometer.…”

Section: Introductionmentioning

confidence: 99%

Non-Contact Real-Time Measurement of Refractive Index of Flowing Liquid Based on Optical Coherence Tomography

Gao

Zhang

et al. 2023

Photonics

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The refractive index (RI) is one of the liquid’s most important physical parameters of liquid. The change of RI usually indicates the change of liquid properties, and this relation leads to broad application. This paper proposes a method based on optical coherence tomography (OCT) to measure the RI of liquid in real time. A series of targeted image-processing techniques play a vital role in this method. Benefiting from the advantages of OCT, this method does not require the operators to come into direct contact with liquids. Moreover, the principles and techniques adopted in this method imply that the measuring range of RI of unknown liquids is unlimited. The effectiveness of this method is validated by the experiment measuring the RI of sucrose liquids with gradient concentration. Another experiment thoroughly verifies this method’s feasibility in predicting sucrose concentration based on real-time measured RI. This simple and easy proposed method indicates an immense potential application prospect in the industry and scientific research.

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In vivo monitoring the dynamic process of acute retinal hemorrhage and repair in zebrafish with spectral‐domain optical coherence tomography

Cited by 11 publications

References 37 publications

Noninvasive Imaging of Cone Ablation and Regeneration in Zebrafish

Noninvasive Imaging of Cone Ablation and Regeneration in Zebrafish

Cytotoxic Evaluation and Anti-Angiogenic Effects of Two Furano-Sesquiterpenoids from Commiphora myrrh Resin

Non-Contact Real-Time Measurement of Refractive Index of Flowing Liquid Based on Optical Coherence Tomography

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