In vivo nanoparticle-mediated radiopharmaceutical-excited fluorescence molecular imaging

Hu, Zhenhua; Qu, Yao; Wang, Kun; Zhang, Xiaojun; Zha, Jiali; Song, Tianming; Bao, Chengpeng; Liu, Haixiao; Wang, Zhongliang; Wang, Jing; Liu, Zhongyu; Liu, Haifeng; Tian, Jie

doi:10.1038/ncomms8560

Cited by 122 publications

(95 citation statements)

References 35 publications

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“…Second, Cerenkov radiation is mainly composed of UV-blue photons which have limited tissue penetration depth. The combination of CR and energy transfer mediators to improve CL intensity is considered as a promising strategy to achieve enhanced PDT outcomes, such as the use of QDs, 138 gold 142 or europium oxide 143 nanoparticles.…”

Section: Depth Penetrationmentioning

confidence: 99%

Reactive oxygen species generating systems meeting challenges of photodynamic cancer therapy

et al. 2016

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Reactive oxygen species (ROS)-mediated mechanism is the major cause underlying the efficacy of photodynamic therapy (PDT). The PDT procedure is based on the cascade of synergistic effects between light, photosensitizer (PS) and oxygen, which greatly favor the spatiotemporal control of the treatment. This procedure has also evoked several unresolved challenges at different levels including (i) limited penetration depth of light restricts traditional PDT to only superficial tumours; (ii) oxygen reliance deprives PDT treatment of hypoxic tumours; (iii) light could complicate the phototherapeutic outcomes due to the concurrent heat generation; (iv) specific delivery of PSs to sub-cellular organelles for exerting effective toxicity remains an issue; and (v) side effects by undesirable white-light activation and self-catalysation of traditional PSs. Recent advances in nanotechnology and nanomedicine have provided new opportunities to develop ROS-generating systems through photodynamic or non-photodynamic procedures while tackling the challenges of current PDT approaches. In this review, we summarize the current status and discuss the possible opportunities of ROS generation for cancer therapy. We hope this review will spur pre-clinical research and clinical practice for ROS-mediated tumour treatment.

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Section: Depth Penetrationmentioning

confidence: 99%

Reactive oxygen species generating systems meeting challenges of photodynamic cancer therapy

et al. 2016

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“…130 To evaluate this concept, three radioactive tracers were used, 18 F-FDG, 99m Tc-MDP (methylene diphosphonate) and 131 I-NaI. 18 FDG emits γ-radiation (511 keV), β + particles and CL, 99m Tc-MDP only emits γ-ray (140 keV), and 131 I-NaI emits major γ-ray (364 keV).…”

Section: Radionuclide-activated Photodynamic Therapymentioning

confidence: 99%

Scintillating Nanoparticles as Energy Mediators for Enhanced Photodynamic Therapy

Kamkaew¹,

Chen²,

et al. 2016

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Achieving effective treatment of deep-seated tumors is a major challenge for traditional photodynamic therapy (PDT) due to difficulties in delivering light into the sub-surface. Thanks to its great tissue penetration, X-rays hold the potential to become an ideal excitation source for activating photosensitizers (PS) that accumulate in deep tumor tissue. Recently, a wide variety of nanoparticles have been developed for this purpose. The nanoparticles are not only designed as carriers for loading various kinds of PSs, but also can facilitate the activation process by transferring energy harvested from X-ray irradiation to the loaded PS. In this review, we focus on recent developments of nanoscintillators with high energy transfer efficiency, their rational designs, as well as potential applications in next generation PDT. Treatment of deep-seated tumors by using radioisotopes as an internal light source will also be discussed.

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“…The EO nanoparticle (Eu 2 O 3 , 99.9% metal basis, molecular weight 5 351.91) was purchased from Aladdin Reagents (Shanghai) Co. Ltd. and used as previously described (24). The excitation spectrum and emission spectrum of EO nanoparticles were recorded with a fluorescence spectrophotometer (F-4500; Hitachi).…”

Section: Preparation Of Radiopharmaceuticals and Eo Nanoparticlesmentioning

confidence: 99%

“…REFT has been explored using our previous work on the radiopharmaceutical-excited fluorescence imaging (REFI) technique, which can dramatically enhance the signal intensity using the dual excitation of nanoparticles by both g-rays and Cerenkov luminescence (CL) (24). When the anatomic information provided by CT is combined, REFT can realize 3D visualization and distribution information and improve the CLT reconstruction by increasing the intensity and signal-to-noise ratio of the surface fluorescent signal.…”

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confidence: 99%

In Vivo 3-Dimensional Radiopharmaceutical-Excited Fluorescence Tomography

Zhao

et al. 2016

J Nucl Med

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Cerenkov luminescence imaging can image radiopharmaceuticals using a high-sensitivity charge-coupled device camera. However, Cerenkov luminescence emitted from the radiopharmaceuticals is weak and has low penetration depth in biologic tissues, which severely limits the sensitivity and accuracy of Cerenkov luminescence imaging. This study presents 3-dimensional (3D) radiopharmaceutical-excited fluorescence tomography (REFT) using europium oxide (EO) nanoparticles, which enhances the Cerenkov luminescence signal intensity, improves the penetration depth, and obtains more accurate 3D distribution of radiopharmaceuticals. Methods: The enhanced optical signals of various radiopharmaceuticals (including Na 131 I, 18 F-FDG, 68 GaCl 3 , Na 99m TcO 4 ) by EO nanoparticles were detected in vitro. The location and 3D distribution of the radiopharmaceuticals of REFT were then reconstructed and compared with those of Cerenkov luminescence tomography through the experiments with the phantom, artificial source-implanted mouse models, and mice bearing hepatocellular carcinomas. Results: The mixture of 68 GaCl 3 and EO nanoparticles possessed the strongest optical signals compared with the other mixtures. The in vitro phantom and implanted mouse studies showed that REFT revealed more accurate 3D distribution of 68 GaCl 3 . REFT can detect more tumors than small-animal PET in hepatocellular carcinoma-bearing mice and achieved more accurate 3D distribution information than Cerenkov luminescence tomography. Conclusion: REFT with EO nanoparticles significantly improves accuracy of localization of radiopharmaceuticals and can precisely localize the tumor in vivo.

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In vivo nanoparticle-mediated radiopharmaceutical-excited fluorescence molecular imaging

Cited by 122 publications

References 35 publications

Reactive oxygen species generating systems meeting challenges of photodynamic cancer therapy

Reactive oxygen species generating systems meeting challenges of photodynamic cancer therapy

Scintillating Nanoparticles as Energy Mediators for Enhanced Photodynamic Therapy

In Vivo 3-Dimensional Radiopharmaceutical-Excited Fluorescence Tomography

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