In vivo near-realtime volumetric optical-resolution photoacoustic microscopy using a high-repetition-rate nanosecond fiber-laser

Shi, Wei; Reza, Parsin Haji; Shao, Peng; Forbrich, Alexander; Zemp, Roger J.

doi:10.1364/oe.19.017143

Cited by 49 publications

(38 citation statements)

References 22 publications

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“…The obliquely positioned unfocused ultrasound transducer further reduces the sensitivity of the photoacoustic part when combined with OCT. Though 2-D fast scanning, using a focusing transducer in its focal spot in the reflection mode OR-PAM has been reported 242 and the small focal spot can be mosaicked to form a larger FOV. 243 It has to use a comparatively low-bandwidth ultrasound transducer for its relatively larger focal spot size, which in turn reduces the achievable axial resolution dramatically that is acoustically determined.…”

Section: Challenges Of Oct/photoacoustic Imagingmentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

432

264

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Abstract. In the last 25 years, optical coherence tomography (OCT) has advanced to be one of the most innovative and most successful translational optical imaging techniques, achieving substantial economic impact as well as clinical acceptance. This is largely owing to the resolution improvements by a factor of 10 to the submicron regime and to the imaging speed increase by more than half a million times to more than 5 million A-scans per second, with the latter one accomplished by the state-of-the-art swept source laser technologies that are reviewed in this article. In addition, parallelization of OCT detection, such as line-field and full-field OCT, has shortened the acquisition time even further by establishing quasi-akinetic scanning. Besides the technical improvements, several functional and contrast-enhancing OCT applications have been investigated, among which the label-free angiography shows great potential for future studies. Finally, various multimodal imaging modalities with OCT incorporated are reviewed, in that these multimodal implementations can synergistically compensate for the fundamental limitations of OCT when it is used alone. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Challenges Of Oct/photoacoustic Imagingmentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

432

264

View full text Add to dashboard Cite

show abstract

“…Recent OR-PAM developments include real-time systems [9,10], small handheld systems [11,12], endoscopy systems [13][14][15], and novel detection schemes [16,17]. Fiber and microchip lasers have recently been introduced as high-repetition-rate sources for realtime OR-PAM [9,18]; however, the wavelength tunability was limited.…”

Section: Introductionmentioning

confidence: 99%

In-Vivo functional optical-resolution photoacoustic microscopy with stimulated Raman scattering fiber-laser source

Reza

Forbrich

Zemp

2014

Biomed. Opt. Express

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Abstract:In this paper a multi-wavelength optical-resolution photoacoustic microscopy (OR-PAM) system using stimulated Raman scattering is demonstrated for both phantom and in vivo imaging. A 1-ns pulse width ytterbium-doped fiber laser is coupled into a single-mode polarization maintaining fiber. Discrete Raman-shifted wavelength peaks extending to nearly 800 nm are generated with pulse energies sufficient for OR-PAM imaging. Bandpass filters are used to select imaging wavelengths. A dualmirror galvanometer system was used to scan the focused outputs across samples of carbon fiber networks, 200μm dye-filled tubes, and Swiss Webster mouse ears. Photoacoustic signals were collected in transmission mode and used to create maximum amplitude projection C-scan images. Double dye experiments and in vivo oxygen saturation estimation confirmed functional imaging potential.

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“…[11][12][13] Optical-resolution photoacoustic microscopy with micron-level lateral resolution demonstrated its ability to image single capillaries. 14,15 Using acoustic-resolution photoacoustic microscopy, high resolution at depths beyond the optical transport mean free path and up to a few centimeters can be achieved with diagnostic frequencies or to depths of a few mm at higher frequencies. To our knowledge, this is the first report on applying photoacoustic imaging to study the angiogenesis induced by temporary implantation of angiocatheters subcutaneously for IT research.…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic imaging of angiogenesis in a subcutaneous islet transplant site in a murine model

et al. 2016

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, "Photoacoustic imaging of angiogenesis in a subcutaneous islet transplant site in a murine model," J. Biomed. Opt. 21(6), 066003 (2016), doi: 10.1117/1.JBO.21.6.066003. Abstract. Islet transplantation (IT) is an established clinical therapy for select patients with type-1 diabetes. Clinically, the hepatic portal vein serves as the site for IT. Despite numerous advances in clinical IT, limitations remain, including early islet cell loss posttransplant, procedural complications, and the inability to effectively monitor islet grafts. Hence, alternative sites for IT are currently being explored, with the subcutaneous space as one potential option. When left unmodified, the subcutaneous space routinely fails to promote successful islet engraftment. However, when employing the previously developed subcutaneous "deviceless" technique, a favorable microenvironment for islet survival and function is established. In this technique, an angiocatheter was temporarily implanted subcutaneously, which facilitated angiogenesis to promote subsequent islet engraftment. This technique has been employed in preclinical animal models, providing a sufficient means to develop techniques to monitor functional aspects of the graft such as angiogenesis. Here, we utilize photoacoustic imaging to track angiogenesis during the priming of the subcutaneous site by the implanted catheter at 1 to 4 weeks postcatheter. Quantitative analysis on vessel densities shows gradual growth of vasculature in the implant position. These results demonstrate the ability to track angiogenesis, thus facilitating a means to optimize and assess the pretransplant microenvironment.

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In vivo near-realtime volumetric optical-resolution photoacoustic microscopy using a high-repetition-rate nanosecond fiber-laser

Cited by 49 publications

References 22 publications

Optical coherence tomography today: speed, contrast, and multimodality

Optical coherence tomography today: speed, contrast, and multimodality

In-Vivo functional optical-resolution photoacoustic microscopy with stimulated Raman scattering fiber-laser source

Photoacoustic imaging of angiogenesis in a subcutaneous islet transplant site in a murine model

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