In vivo nonlinear imaging of corneal structures with special focus on BALB/c and streptozotocin-diabetic Thy1-YFP mice

Ehmke, Tobias; Leckelt, Janine; Reichard, Maria; Weiß, H.; Hovakimyan, Marina; Heisterkamp, Alexander; Stachs, Oliver; Baltrusch, Simone

doi:10.1016/j.exer.2015.11.024

Cited by 12 publications

(11 citation statements)

References 30 publications

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“…Use of special imaging techniques such as 2-photon microscopy in genetically modified mice with visible corneal nerves will be immensely valuable to investigate changes in animals with diabetic neuropathy, especially in the cornea. 7 …”

Section: Conclusion and Future Studiesmentioning

confidence: 99%

“…1 However, new research beyond 2008 has not been systematically reviewed, even after the emergence of fairly recent review articles. 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 This is an important issue to address as new developments such as cellular, molecular biology and animal genetics have advanced considerably in the last few years. Here, we provide a systematic review of the recent literature (published 2009–2015), which enlightens on the role of the ocular surface and cornea in DM (Figure 1) and research on potential treatment strategies.…”

Section: Introductionmentioning

confidence: 99%

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A systematic review on the impact of diabetes mellitus on the ocular surface

2017

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Diabetes mellitus is associated with extensive morbidity and mortality in any human community. It is well understood that the burden of diabetes is attributed to chronic progressive damage in major end-organs, but it is underappreciated that the most superficial and transparent organ affected by diabetes is the cornea. Different corneal components (epithelium, nerves, immune cells and endothelium) underpin specific systemic complications of diabetes. Just as diabetic retinopathy is a marker of more generalized microvascular disease, corneal nerve changes can predict peripheral and autonomic neuropathy, providing a window of opportunity for early treatment. In addition, alterations of immune cells in corneas suggest an inflammatory component in diabetic complications. Furthermore, impaired corneal epithelial wound healing may also imply more widespread disease. The non-invasiveness and improvement in imaging technology facilitates the emergence of new screening tools. Systemic control of diabetes can improve ocular surface health, possibly aided by anti-inflammatory and vasoprotective agents.

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Section: Conclusion and Future Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A systematic review on the impact of diabetes mellitus on the ocular surface

2017

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“…Darstellung des subbasalen Nervenplexus mittels 2-Photonen-Mikroskopie bei der thy1-YFP-Maus ! Um eine genauere und die CLSM ergänzende Untersuchung des subbasalen Nervenplexus in vivo zu erreichen, haben wir uns des Modells der thy1-YFP-Maus (B6.Cg-Tg(Thy1-YFP)16Jrs/J) bedient [31,32]. In diesen Mäusen wird das gelb fluoreszierende Protein (YFP) unter Kontrolle von neuronalen regulativen Elementen des Thy1-Gens der Maus gebildet.…”

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“…In der Fluoreszenzmikroskopie sind die Axone an der lebenden thy1-YFP-Maus vollständig sichtbar [33]. Die nicht lineare Lasermikroskopie eignet sich hervorragend, um die Struktur der Kornea mit ihren Schichten abzubilden [31,34]. Während sich Endothel und Epithel aufgrund ihrer Autofluoreszenz mittels 2-Photonen-Mikroskopie darstellen lassen, liefert das Kollagen im Stroma ein Signal in der 2.…”

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Ophthalmologisches Monitoring der diabetischen Neuropathie am Mausmodell

Baltrusch

2016

Klin Monatsbl Augenheilkd

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Patients with diabetes mellitus suffer from late damage, including microvascular and macrovascular complications and diabetic polyneuropathy, even though blood glucose is well controlled and the HbA1c value normalised. Peripheral nerves are affected, and when these are damaged, this can result in disturbed sensation and pain. Such nerves are mainly Aδ und C fibres, which are present in the skin, but also in the subbasal nerve plexus of the cornea. Imaging of the subbasal nerve plexus using microscopic techniques allows a very early and objective view of neuropathy, which is only possible invasively by skin biopsy. We have used a thy1-YFP mouse strain in which the peripheral nerves are detectable by fluorescence excitation. Using two photon microscopy, we produced high resolution three dimensional images of the corneal subbasal nerve plexus of these mice. We induced diabetes mellitus in thy1-YFP mice by injection of streptocotocin. We showed that the increase in blood glucose was accompanied by a loss of subbasal nerve fibres, mainly in the centre of the cornea. Subsequent treatment of the animals with insulin normalised blood glucose and was accompanied by an increase in subbasal nerve fibres. Thus, this model is highly suited to investigate the pathogenesis of diabetic polyneuropathy, with the aim of developing new causal strategies to treat the disease.

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