In vivo observation of transient photoreceptor movement correlated with oblique light stimulation

Abstract

Rod-dominated transient retinal phototropism (TRP) has been observed in freshly isolated retinas, promising a noninvasive biomarker for high resolution assessment of retinal physiology. However, in vivo mapping of TRP is challenging due to its fast time course and sub-cellular signal magnitude. By developing a line-scanning and virtually structured detection based super-resolution ophthalmoscope, we report here in vivo observation of TRP in frog retina. In vivo characterization of TRP time course and magnitude were implemented by using variable light stimulus intensities.

In vivo super-resolution imaging of transient retinal phototropism evoked by oblique light stimulation

“…Temporal dynamics of in vivo TRP correlated with variable stimulus intensities was characterized to verify the physiological origin of TRP. 12 Adult northern leopard frogs (Rana Pipiens) were used for this study. The frogs were first dark-adapted for at least 4 h prior to in vivo imaging and then anesthetized through the skin.…”