‘In vivo’ optical approaches to angiogenesis imaging

Snoeks, T; Löwik, Clemens W.G.M.; Kaijzel, Eric L.

doi:10.1007/s10456-010-9168-y

Cited by 33 publications

(31 citation statements)

References 89 publications

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“…64 Depending on the imaging strategy, optical filters can select for both excitation and emission wavelengths as required. Although filters are primarily used for fluorescence imaging, they can also distinguish between luminescent reporter signals and for determining the depth of a luminescent source within a subject.…”

Section: Instrumentation For Oimentioning

confidence: 99%

“…The ability of Magnetospirillum magneticum AMB-1 to confer positive MRI contrast was determined both in vitro and in vivo. 102 The strain was administered to tumor bearing mice either intra-tumorally or intravenously and tumor targeting was confirmed using a multiple of approaches including 64 Cu-labeled bacteria. Significantly M. magneticum with small magnetite particles generate T1-weighted positive contrast, enhancing in vivo visualization by MRI.…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

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Bacterial vectors for imaging and cancer gene therapy: a review

et al. 2012

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The significant burden of resistance to conventional anticancer treatments in patients with advanced disease has prompted the need to explore alternative therapeutic strategies. The challenge for oncology researchers is to identify a therapy which is selective for tumors with limited toxicity to normal tissue. Engineered bacteria have the unique potential to overcome traditional therapies' limitations by specifically targeting tumors. It has been shown that bacteria are naturally capable of homing to tumors when systemically administered resulting in high levels of replication locally, either external to (non-invasive species) or within tumor cells (pathogens). Pre-clinical and clinical investigations involving bacterial vectors require relevant means of monitoring vector trafficking and levels over time, and development of bacterial-specific real-time imaging modalities are key for successful development of clinical bacterial gene delivery. This review discusses the currently available imaging technologies and the progress to date exploiting these for monitoring of bacterial gene delivery in vivo.Cancer Gene Therapy ( INTRODUCTIONAlthough the potential anti-cancer effects of acquired bacterial infection have been evident for over 120 years and the presence of bacteria in excised tumors has been noted for over 60 years, 1 it is only in more recent times that the tumor-specific growth of bacteria has been considered for therapeutic purposes. While the precise mechanism(s) behind preferential bacterial tumor colonization remain poorly understood, this phenomenon must relate to unique tumor attributes that separate them from healthy tissues. Factors such as the irregular blood supply; local immune suppression; inflammation; and unique nutrient supply (e.g., purines) in tumors have been proposed to play a role. 2 Bacterial entry into the tumor environment is proposed to be facilitated by the leaky vasculature. Cancer cells are characterized by an aberrantly accelerated metabolism and proliferation leading to an imbalance of oxygen supply and consumption which is a major causative factor of tumor hypoxia. 3 The hypoxic nature of solid tumors enhances the growth of anaerobic and facultatively anaerobic bacteria. In addition, these areas with low oxygen and high interstitial pressure are considered to be an immunological sanctuary, where bacterial clearance mechanisms are greatly inhibited. 4 Necrotic regions are also rich in nutrients favoured by bacteria due to tumor cell turnover. However, tumor selective bacterial colonization now appears to be both bacterial species and tumor origin independent, since aerobic bacteria are also capable of colonising tumors, and even small tumors lacking an anaerobic center are colonised. See Figure 1 for proposed mechanisms.Invasive bacteria can internalize and replicate within tumor cells, while non-invasive bacteria grow externally to tumor cells, within the tumor micro-environment. 5 The tumor selective growth of bacteria has made them an attractive vehicle for the delivery of ...

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Section: Instrumentation For Oimentioning

confidence: 99%

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

Bacterial vectors for imaging and cancer gene therapy: a review

et al. 2012

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“…Bioluminescence imaging (BLI), especially, demonstrates the most desirable combination of high sensitivity and easy handling (5) and is suitable for acquire signal deep in tissue (6). It has been widely used for monitoring different cancer biological processes [angiogenesis (7,8), infinite proliferation and meta stasis (9,10), multidrug resistance (11,12)] and assessing the efficacy of different therapeutic modalities (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Real-time bioluminescence and tomographic imaging of gastric cancer in a novel orthotopic mouse model

Liu

Yao

et al. 2012

Oncol Rep

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Abstract. Gastric cancer is the second leading cause of cancer mortality worldwide. Understanding the multistep process of carcinogenesis of gastric cancer is pivotal to develop novel therapeutic strategies. Molecular imaging in preclinical cancer models bridges the gap of laboratory-based experiment and clinical translation. To this end, the human gastric cancer cell line SGC-7901 was established to stably express luciferase and GFP by lentiviral transduction (SGC7901-Luc-GFP). Preclinical models were developed by orthotopic transplantation of SGC-7901-Luc-GFP into the sub-serosal layer of the stomach of immunocompromised mice. Tumor progression and therapeutic responses were dynamically tracked by bioluminescence imaging (BLI). Bioluminescence tomography (BLT) was used to monitor stereoscopic morphological and signal changes during tumor progression. Good correlation between cell number and bio luminescence/ fluorescence intensity was observed (R 2 =0.9983/r 2 =0.9974) in vitro. Tumor progression and therapeutic response could be successfully followed directly by BLI. Importantly, BLT provided a more accurate spatial location and tomographic quantification of the internal lesion. In conclusion, our novel bioluminescence-based preclinical gastric cancer models enable superior, noninvasive monitoring gastric cancer progression and their drug responses. The BLT technique in particular, may have great potential for future oncological studies.

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“…Since a v b 3 integrins recognize specific component of the extracellular matrix containing the arginine-glycine-aspartic acid (RGD), RGDcontaining peptides have also been developed as targeting ligands for imaging neo-vasculature formation in tumours; indeed, radiolabelled RGDcontaining compounds such as ( 18 )F-Galacto-RGD or ( 99m )Tc-NC100692 have been successfully used for PET analysis of the neo-angiogenesis associated to squamous cell carcinoma or breast cancer (BachGansmo et al 2006, Beer et al 2007. Bioluminescence modality is also often applied for the detection of new vessel formation (Fukumura et al 1998, Snoeks et al 2010; in this research line, an interesting transgenic mouse model was recently developed as a tool to follow the activation of the hypoxia-inducible factor1a (Hif1a). Hif1a is a transcription factor turning on the neo-angiogenesis programme when cells are under low-oxygen conditions.…”

Section: Angiogenesismentioning

confidence: 99%

Novel insights on imaging sex hormone-dependent tumourigenesis in vivo

Ramachandran

Stell²,

Maravigna³

et al. 2011

Endocrine-Related Cancer

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Sex hormones modulate proliferation, apoptosis, migration, metastasis and angiogenesis in cancer cells influencing tumourigenesis from the early hyperplastic growth till the end-stage metastasis. Although decades of studies have detailed these effects at the level of molecular pathways, where and when these actions are needed for the growth and progression of hormonedependent neoplasia is poorly elucidated. Investigation of the hormone influences in carcinogenesis in the spatio-temporal dimension is expected to unravel critical steps in tumour progression and in the onset of resistance to hormone therapies. Non-invasive in vivo imaging represents a powerful tool to follow in time hormone signalling in the whole body during tumour development. This review summarizes the tools currently available to follow hormone action in living organisms.

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‘In vivo’ optical approaches to angiogenesis imaging

Cited by 33 publications

References 89 publications

Bacterial vectors for imaging and cancer gene therapy: a review

Bacterial vectors for imaging and cancer gene therapy: a review

Real-time bioluminescence and tomographic imaging of gastric cancer in a novel orthotopic mouse model

Novel insights on imaging sex hormone-dependent tumourigenesis in vivo

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