In-vivo optogenetics and pharmacology in deep intracellular recordings

Katz, Yonatan; Sokoletsky, Michael; Lampl, Ilan

doi:10.1016/j.jneumeth.2019.108324

Cited by 14 publications

(11 citation statements)

References 34 publications

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“…1B, see methods). Direct and indirect pathway MSNs were determined by their responses to the light stimulation using the optopatcher (Katz et al, 2019;Ketzef et al, 2017) (Fig. 7A).…”

Section: Msns In the Dls And Dms Have Different Electrophysiological mentioning

confidence: 99%

Medium spiny neurons activity reveals the discrete segregation of mouse dorsal striatum

Alegre-Cortés

Sáez

Montanari

et al. 2020

Preprint

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AbstractBehavioural studies differentiate the rodent dorsal striatum (DS) into lateral and medial regions based on their particular roles; however, anatomical evidence suggest that it is a unified structure. To understand striatal dynamics and basal ganglia functions, it is essential to clarify the circuitry that supports this behavioural-based segregation. Here, we show that the mouse DS is made of two non-overlapping functional circuits divided by a sharp boundary. Combining in vivo optopatch-clamp and extracellular recordings of spontaneous activity, we demonstrate different coupling of lateral and medial striatum to the cortex together with an independent integration of the slow and fast oscillations, due to particular cortico-striatal connectivity and local properties of each region. Additionally, distinct properties between striatonigral and striatopallidal neurons were revealed along DS. These results demonstrate that the rodent DS is segregated in two neuronal circuits, in homology with the caudate and putamen nuclei of primates.

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“…1B, see methods). Direct and indirect pathway MSNs were determined by their responses to the light stimulation using the optopatcher (Katz et al, 2019;Ketzef et al, 2017) (Fig. 7A).…”

Section: Msns In the Dls And Dms Have Different Electrophysiological mentioning

confidence: 99%

Medium spiny neurons activity reveals the discrete segregation of mouse dorsal striatum

Alegre-Cortés

Sáez

Montanari

et al. 2020

Preprint

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“…In the longer term, optogenetics might be an approach for greater specificity in neural actuation. 37 Neurons are modified to express light-sensitive opsins and then subsequently controlled by light. 37 The potential therapeutic advantage of optogenetics is the specificity to cell types, the ability to modulate different cell types with different wavelengths, and the capability to directly inhibit or excite activity.…”

Section: Optimizing Stimulation Methodsmentioning

confidence: 99%

Neuromodulation in 2035

Denison

Morrell

2022

Neurology

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Neuromodulation devices are approved in the United States for the treatment of movement disorders, epilepsy, pain, and depression, and are used off-label for other neurologic indications. By 2035, advances in our understanding of neuroanatomical networks and in the mechanism of action of stimulation, coupled with developments in material science, miniaturization, energy storage, and delivery, will expand the use of neuromodulation devices. Neuromodulation approaches are flexible and modifiable. Stimulation can be targeted to a dysfunctional brain focus, region, or network, and can be delivered as a single treatment, continuously, according to a duty cycle, or in response to physiologic changes. Programming can be titrated and modified based on the clinical response or a physiologic biomarker. In addition to keeping pace with clinical and technological developments, neurologists in 2035 will need to navigate complex ethical and economic considerations to ensure access to neuromodulation technology for a rapidly expanding population of patients. This article provides an overview of systems in use today and those that are anticipated and highlights the opportunities and challenges for the future, some of which are technical, but most of which will be addressed by learning about brain networks, and from rapidly growing experience with neuromodulation devices.

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“…New techniques now integrate different functionalities in individual glass pipettes to expand the toolbox of methods, including optogenetics, pharmacology or electrochemistry, that can be applied for single-cell analysis (Hunt et al, 2019;Katz et al, 2013;Packer et al, 2015; see also Katz et al, 2019). Collecting different samples of individual celltypes in vivo is time consuming though, which intrinsically limits the application of the technique.…”

Section: Emerging Technological Approachesmentioning

confidence: 99%