In vivo pharmacokinetic comparisons of ferulic acid and puerarin after oral administration of monomer, medicinal substance aqueous extract and Nao-De-Sheng to rats

Abstract

Background:Nao-De-Sheng decoction (NDS), a traditional Chinese medicine (TCM) prescription containing Radix puerariae lobatae, Floscarthami, Radix et Rhizoma Notoginseng, Rhizoma chuanxiong and Fructus crataegi, is effective in the treatment of cerebral arteriosclerosis, ischemic cerebral stroke and apoplexy linger effect. Ferulic acid and puerarin are the main absorbed effective ingredients of NDS.Objective:To assess the affection of other components in medical material and compound recipe compatibility on the pharmacokinetics of ferulaic acid and puerarin, of ferulic acid from the monomer Rhizoma chuanxiong aqueous extract and NDS were studied. And pharmacokinetics comparisons of puerarin from the monomer Radix puerariae extract and NDS decoction were investigated simultaneously.Materials and Methods:At respective different time points after oral administration of the monomer, medicinal substance aqueous extract and NDS at the same dose in rats, plasma concentrations of ferulic acid and puerarin in rats were determined by RP-HPLC, and the main pharmacokinetic parameters were estimated with 3P97 software.Results:The plasma concentration-time curves of ferulaic acid and puerarin were both best fitted with a two-compartment model. AUC0−t, AUC0→∞, Tmax, and Cmax of ferulic acid in the monomer and NDS decoction were increased significantly (P < 0.05) compared with that in Rhizoma chuanxiong aqueous extract. And statistically significant increase (P < 0.05) in pharmacokinetic parameters of puerarin including AUC0−t, AUC0→∞, CL, Tmax and Cmax were obtained after oral administration of puerarin monomer compared with Radix puerariae extract. Although the changes of AUC0−t, AUC0→∞ and CL had no statistically significant, Cmax of puerarin in NDS was increased remarkably (P < 0.05) compared with that in single puerarin.Conclusions:Some ingredients of Rhizoma chuanxiong and Radix puerariae may be suggested to remarkably influence plasma concentrations of ferulaic acid and puerarin. Some ingredients in NDS may increase dissolution and absorption of ferulaic acid and puerarin, delay elimination, and subsequently enhance bioavailability of ferulaic acid and puerarin in rats after compatibility.

Pharmacokinetics and drug delivery systems for puerarin, a bioactive flavone from traditional Chinese medicine

“…Species specificity was found in pharmacokinetics of puerarin. The pharmacokinetics of puerarin in plasma of rats and dogs coincides with the two-compartment open model (Jin & Zhu, 1992; Yang et al., 2011; Ouyang et al., 2012), while in rabbits was best fitted to the three-compartment open model (Jin & Zhu, 1992).…”

Pharmacokinetics and tissue distribution study of tanshinone IIA after oral administration of Bushen Huoxue Qubi granules to rats with blood stasis syndrome

“…Several pharmacokinetic and tissue distribution studies focus on normal animal,[ 14 ] regardless of pathological condition. Our study showed that blood stasis was associated with significantly higher AUC, C max , t 1/2 and lower CL and Vd of tanshinone IIA in plasma and higher AUC 0-t of tanshinone IIA in the analyzed tissues of rats treated with BHQ.…”

Effects of puerarin combined with conventional therapy on ischemic stroke

“…Ischemic stroke has a very acute onset, and if not treated immediately, it often leads to severe disability or death. This disease occurs commonly in elderly patients, in whose morbidity and mortality rates rank first among neurological diseases ( 12 ). Ischemic stroke is mainly due to cerebral arterial atherosclerosis.…”