In vivo pharmacological investigation of Monotheca buxifolia and Bosea amherstiana using animal models

Hassan, Said; Ahmad, Bashir; Khan, Shahid Ullah; Huang, Linfang; Anjum, Syed Ishtiaq; Ansari, Muhammad Javed; Rahman, Khaista; Ahmad, Iftikhar; Khan, Wasim Ullah; Qamar, Rabia; Man, Shad; Nabi, Ghulam; Shah, Ayesha Haleem

doi:10.1016/j.sjbs.2018.10.008

Cited by 5 publications

(9 citation statements)

References 15 publications

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“…Infrared spectra (IR) were recorded on Shimadzu IR-460 in nujol or KBr pellets and reported in cm −1 . NMR spectra were recorded on Bruker AMX-500 and AM-400 for 1 H (300–500 MHz) and 13 C (125–150 MHz) spectrometers. "spectra were recorded on Bruker Avance spectrometers ranging from 7.05 up to 14.09 T".…”

Section: Methodsmentioning

confidence: 99%

“…is monotypic genus of the family Sapotacea, grows mainly in Malakand Dir district, Pakistan. M. buxifolia is reported for wide-range of pharmacological activities including cough, wound healing, headache, analgesic, antipyretic, antiseptic and urinary tract infections 13 . The antibacterial activity of crude ethanolic extract and fractions of M. buxifolia using agar well diffusion technique confirmed their antibacterial potential 14 .…”

Section: Introductionmentioning

confidence: 99%

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Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

Javed

Mahmood

Khan

et al. 2021

Sci Rep

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Antifungal activity of Monothecabuxifolia methanolic extract and its various fractions were assessed against Macrophominaphaseolina, a soil-borne fungal pathogen of more than 500 vegetal species as well as rare and emerging opportunistic human pathogen. Different concentrations of methanolic extract (3.125 to 200 mg mL−1) inhibited fungal biomass by 39–45%. Isolated n-hexane, chloroform and ethyl acetate fractions suppressed fungal biomass by 32–52%, 29–50% and 29–35%, respectively. Triterpenes lupeol and lupeol acetate (1, 2) were isolated from n-hexane while betulin, β-sitosterol, β-amyrin, oleanolic acid (3–6) were isolated from chloroform fraction. Vanillic acid, protocatechuic acid, kaempferol and quercetin (7–10) were isolated from the ethyl acetate fraction and identified using various spectroscopic techniques namely mass spectroscopy and NMR. Antifungal activity of different concentrations (0.0312 to 2 mg mL−1) of the isolated compounds was evaluated and compared with the activity of a broad spectrum fungicide mancozeb. Different concentrations of mencozeb reduced fungal biomass by 83–85%. Among the isolated compounds lupeol acetate (2) was found the highest antifungal against M.phaseolina followed by betulin (3), vanillic acid (7), protocatechuic acid (8), β-amyrin (5) and oleanolic acid (6) resulting in 79–81%, 77–79%, 74–79%, 67–72%, 68–71% and 68–71%, respectively. Rest of the compounds also showed considerable antifungal activity and reduced M.phaseolina biomass by 41–64%.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

Javed

Mahmood

Khan

et al. 2021

Sci Rep

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“…Fruit of S. mascatense was also found to have some antibacterial properties (Ullah et al, 2017). Methanolic extracts from aerial portions of S. mascatense were found to reduce pain, fever, and inflammation (Hassan et al, 2019). Burki et al (2018) found that extracts of S. mascatense bark had anti-inflammatory and antidepressant properties.…”

Section: Existing and Potential Uses And Benefitsmentioning

confidence: 99%

Sideroxylon mascatense: A new crop for high elevation arid climates?

Hopkins

Al-Yahyai

2020

Jour. Agri. & Mar. Scie.

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This paper reviews the existing research on Sideroxylon mascatense, and provides an argument for being considered a fruit crop in cultivated production systems in the Sultanate of Oman and elsewhere. Climate change due to global warming has adverse effects on the agro-ecosystems of mountain regions in marginal climate zones. For example, in the Western Hajar Mountains of Oman, yields of the temperate fruit crops have decreased in recent years as temperature and other climate anomalies have increased. Other fruit-bearing wild plants have also been impacted by extreme weather fluctuations, particularly those that occupy a narrow altitudinal range. One such plant is S. mascatense, a currently underutilized fruiting plant found growing in the wild all through the Middle East and other arid mountainous regions. Two fruiting types of S. mascatense are found in Oman, both of which are seasonally wild harvested by mountain inhabitants and sold in markets as well as along the roadsides. While some specimens exist in cultivated areas, propagation and new plantings are non-existent. Regeneration in the wild is also in decline in Oman, possibly due to climate change. Increasing S. mascatense populations could be achieved via propagation, as well as commercial cultivation, although further research is needed into cropping systems and best practice methods.

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“…Monotheca buxifolia species founds in hilly areas of Pakistan which has therapeutic uses such as antiseptic, relieving gastrointestinal disorders, analgesic, anti-inflammatory, antipyretic, and wound healings [14]. These plants are found in abundance in Dir Lower, Attock, Xhob Kala Chitta, and Chitral [15].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Compounds from <em>Monotheca Buxifolia</em> Inhibit the Growth of Hepatocellular Carcinoma Cells

Hassan¹,

Rehman²,

Jamal³

et al. 2020

Preprint

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The natural products and conventional chemotherapeutic drugs are believed to increase the cure rates of anti-cancer treatment while reducing their toxicity. The current study investigates the cytotoxic and apoptogenic effects of bioactive compounds from Monotheca buxifolia on Hep G2 cell lines. The effect on the viability of Hep G2 cells was evaluated by MTT assay; Morphological changes were studied, the apoptotic activity was demonstrated through Annexin-V-FITC/ PI, a molecular dynamics simulation study was conducted to explore the binding pattern of the compounds in the active site of the PPRAδ protein. The isolated compounds lauric acid, oleanolic acid, and bis(2-ethylhexyl) phthalate inhibited the growth of hepatocellular cancer cells, as determined by MTT assay and annexin V-FITC/PI. The IC50 value for lauric acid was 56.4584 ± 1.20 µg/ml, that for oleanolic acid was 31.9421 ± 1.03 µg/ml, and that for bis(2-ethylhexyl) phthalate was 83.8019 ± 2.18 µg/ml. After 24 h of treatment, 29.5% of Hep G2 cells treated with lauric acid, 52.1% of those treated with oleanolic acid, and 22.4% of those treated with bis(2-ethylhexyl) phthalate were apoptotic. Morphological assay and Hoechst staining microscopy revealed the morphological alterations of cell membrane accompanied by nuclear condensation after treatment. The high fluctuation indicates the high potency and adopting various interactions, and vice versa, the oleanolic acid showed highly residues fluctuation, which remains stable in the active site of PPARδ protein and involved in various interactions while remaining locally fluctuated in the binding site the other two compounds. In conclusion, a significant apoptogenic effect was exhibited by lauric acid, oleanolic acid, and bis(2-ethylhexyl) phthalate against HepG2 cells in inducing apoptosis. Our findings indicate that these bioactive compounds hold promise as potential therapeutic for hepatocellular carcinoma.

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In vivo pharmacological investigation of Monotheca buxifolia and Bosea amherstiana using animal models

Cited by 5 publications

References 15 publications

Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

Sideroxylon mascatense: A new crop for high elevation arid climates?

Bioactive Compounds from <em>Monotheca Buxifolia</em> Inhibit the Growth of Hepatocellular Carcinoma Cells

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