In vivo photoacoustic imaging of cancer using indocyanine green-labeled monoclonal antibody targeting the epidermal growth factor receptor

Sano, Kohei; Ohashi, Manami; Kanazaki, Kengo; Ding, Ning; Deguchi, Jun; Kanada, Yuko; Ono, Masahiro; Saji, Hideo

doi:10.1016/j.bbrc.2015.07.042

Cited by 31 publications

(20 citation statements)

References 22 publications

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“…Importantly, unlike fluorescent imaging, such FA‐targeted ICG‐PLGA‐lipid NPs could successfully delineate the tumor region of MCF‐7 breast carcinoma in vivo visualized by ultrasound images and provide depth‐resolved information (Figure c). Later, in another study, Sano et al labeled ICG with a monoclonal antibody against human epidermal growth factor receptor (EGFR) to enhance target‐specific PA signal in EGFR‐positive cells . Most recently, optimization of ICG confinement in pores of pegylated amino‐mesoporous silica NPs has revealed approx.…”

Section: Cyanine Nanoprobes In Image Guided Cancer Theranosticsmentioning

confidence: 99%

Cyanine based Nanoprobes for Cancer Theranostics

Bhattarai

Dai

2017

Adv Healthcare Materials

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Cyanine dyes are greatly accredited in the development of non‐invasive therapy that can “see” and “treat” tumor cells via imaging, photothermal and photodynamic treatment. However, these dyes suffer from poor pharmacokinetics inducing severe toxicity to normal cells, insufficient accumulation in tumor regions and rapid photobleaching when delivered in free forms. Nanoparticles engineered to encapsulate these compounds and delivering them into tumor regions have increased rapidly, however, so far, these nanoparticles (NPs) have not proved to be so effective to circumvent existing challenges. Newly designed multifunctional smart nanocarriers that can improve phototherapeutic properties of these dyes, co‐encapsulate multiple potent therapeutic compounds, and simultaneously overcome limitations related to tumor recurrence, metastases, limited intracellular uptake, and tumor hypoxia have potential to revolutionize modern paradigm of cancer therapy. Such cyanine based multifunctional nanocarriers integrating imaging and therapy in a single platform can effectively produce better clinical outcomes in cancer treatment. This review briefly summarizes recent advancements of cyanine nanoprobes that are currently used as imaging/phototherapeutic agents in unimodal/bimodal/trimodal cancer theranostics. Finally, we conclude this review by addressing challenges of pre‐existing therapeutic systems and designs adopted to overcome them with a brief insight assimilating future perspective of emerging cyanine‐based NPs in cancer theranostics.

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Section: Cyanine Nanoprobes In Image Guided Cancer Theranosticsmentioning

confidence: 99%

Cyanine based Nanoprobes for Cancer Theranostics

Bhattarai

Dai

2017

Adv Healthcare Materials

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“…Because of its biocompatibility 9 , PA probes labeled with indocyanine green (ICG), a US Food and Drug Administration (FDA)-approved near-infrared fluorescence dye, have been investigated 10 . In our previous studies, the feasibility of using proteins and polyethylene glycol (PEG) conjugated with ICG for tumor-targeted PA probes has been demonstrated 11 12 13 . PEG with large molecular weight (>20 kDa) accumulated in the tumor via enhanced permeability and retention (EPR) effect 14 15 , and visualization of tumors was achieved in PA imaging.…”

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confidence: 99%

Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors

Kanazaki

Sano

Makino

et al. 2016

Sci Rep

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Photoacoustic imaging, which enables high-resolution imaging in deep tissues, has lately attracted considerable attention. For tumor imaging, photoacoustic probes have been proposed to enhance the photoacoustic effect to improve detection sensitivity. Here, we evaluated the feasibility of using a biocompatible hydrophilic polymer, polyoxazoline, conjugated with indocyanine green (ICG) as a tumor-targeted photoacoustic probe via enhanced permeability and retention effect. ICG molecules were multivalently conjugated to partially hydrolyzed polyoxazoline, thereby serving as highly sensitive photoacoustic probes. Interestingly, loading multiple ICG molecules to polyoxazoline significantly enhanced photoacoustic signal intensity under the same ICG concentration. In vivo biodistribution studies using tumor bearing mice demonstrated that 5% hydrolyzed polyoxazoline (50 kDa) conjugated with ICG (ICG/polyoxazoline = 7.8), P14-ICG7.8, showed relatively high tumor accumulation (9.4%ID/g), resulting in delivery of the highest dose of ICG among the probes tested. P14-ICG7.8 enabled clear visualization of the tumor regions by photoacoustic imaging 24 h after administration; the photoacoustic signal increased in proportion with the injected dose. In addition, the signal intensity in blood vessels in the photoacoustic images did not show much change, which was attributed to the high tumor-to-blood ratios of P14-ICG7.8. These results suggest that polyoxazoline-ICG would serve as a robust probe for sensitive photoacoustic tumor imaging.

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“…LLC.) [ [29] , [30] ] and ICG (081104215, concentration: 2500 kg/m 3 (3225 mM), peak absorption: 780 nm, hepatic function test, Daiichi Sankyo Company, Limited) [ [31] , [32] , [33] ]. Like the first two, the IR780 agent (425311, concentration: 1000 kg/m 3 (1500 mM), peak absorption: 795 nm, Sigma-Aldrich Co.…”

Section: Methodsmentioning

confidence: 99%

“…Our aim is to identify sentinel lymph nodes in the early stage of breast cancer by visualizing the path from the tumor to the node with a contrast agent (e.g. gold nanorods (AuNR) [ [29] , [30] ], ICG [ [31] , [32] , [33] ], or EB [ [26] , [27] , [28] ]). In this paper, we describe our studies on visualizing lymph vessels.…”

Section: Introductionmentioning

confidence: 99%

Visualization of murine lymph vessels using photoacoustic imaging with contrast agents

et al. 2018

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Metastasis frequently occurs even in the early stage of breast cancer. This research studied the feasibility of using photoacoustic (PA) imaging for identifying metastasis in the lymph vessels of mice. The photoacoustic efficiency of various contrast agents was investigated, and the influence of scattered light was evaluated by using a lymph vessel phantom. The lymph vessels of mice were then visualized using the selected contrast agents: indocyanine green (ICG) and gold nanorods (AuNR). The attenuation of the PA imaging was −1.90 dB/mm, whereas that of the fluorescence imaging was −4.45 dB/mm. The results indicate the potential of identifying sentinel lymph nodes by using PA imaging with these contrast agents.

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In vivo photoacoustic imaging of cancer using indocyanine green-labeled monoclonal antibody targeting the epidermal growth factor receptor

Cited by 31 publications

References 22 publications

Cyanine based Nanoprobes for Cancer Theranostics

Cyanine based Nanoprobes for Cancer Theranostics

Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors

Visualization of murine lymph vessels using photoacoustic imaging with contrast agents

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