In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light

Ran, Chongzhao; Zhang, Zhaoda; Hooker, Jacob M.; Moore, Anna

doi:10.1007/s11307-011-0489-z

Cited by 80 publications

(79 citation statements)

References 22 publications

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“…Beta-emitting radionuclides have the potential to deliver blue/UV light deep inside the body via their Cerenkov emission, and using targeted radiotracers it may be possible to deliver that light to quite specific locations. First studies on the use of radiotracer-delivered Cerenkov light for photoactivation have already been published [25].…”

Section: Discussionmentioning

confidence: 99%

In vivo Cerenkov luminescence imaging: a new tool for molecular imaging

Mitchell

Gill

Boucher

et al. 2011

Phil. Trans. R. Soc. A.

152

189

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Cerenkov radiation is a phenomenon where optical photons are emitted when a charged particle moves faster than the speed of light for the medium in which it travels. Recently, we and others have discovered that measurable visible light due to the Cerenkov effect is produced in vivo following the administration of b-emitting radionuclides to small animals. Furthermore, the amounts of injected activity required to produce a detectable signal are consistent with small-animal molecular imaging applications. This surprising observation has led to the development of a new hybrid molecular imaging modality known as Cerenkov luminescence imaging (CLI), which allows the spatial distribution of biomolecules labelled with b-emitting radionuclides to be imaged in vivo using sensitive charge-coupled device cameras. We review the physics of Cerenkov radiation as it relates to molecular imaging, present simulation results for light intensity and spatial distribution, and show an example of CLI in a mouse cancer model. CLI allows many common radiotracers to be imaged in widely available in vivo optical imaging systems, and, more importantly, provides a pathway for directly imaging b − -emitting radionuclides that are being developed for therapeutic applications in cancer and that are not readily imaged by existing methods.

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Section: Discussionmentioning

confidence: 99%

In vivo Cerenkov luminescence imaging: a new tool for molecular imaging

Mitchell

Gill

Boucher

et al. 2011

Phil. Trans. R. Soc. A.

152

189

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show abstract

“…The measured radiance L meas will be dependent on the quantum efficiency Q e of the detector, more precisely L meas ðλÞ ¼ LðλÞQ e ðλÞ: (11) Whereas in this case the dependence by the wavelength λ has been explicitly included in the equation, the wavelength dependence of the tissue μ eff was also taken into account in order to estimate the surface radiance. The values of μ eff at different wavelengths were obtained considering the optical properties of mouse muscle.…”

mentioning

confidence: 99%

“…21,22 In order to investigate the differences in the measured radiance, the Q e profiles of two different commercial CCD were included in Eq. (11).…”

mentioning

confidence: 99%

Optimizing in vivo small animal Cerenkov luminescence imaging

Spinelli

Boschi

2012

J. Biomed. Opt.

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“…Estimates of this quantity are particularly important in guiding the development of future imaging systems and biophotonics applications utilizing Cherenkov radiation, particularly with the emerging interest in using Cherenkov radiation for low fluence threshold phototherapy (Ran et al, 2012, Gonzales et al, 2014, Kotagiri et al, 2015, Hartl et al, 2015. Herein we develop a robust and flexible methodology for modeling both the radiation and optical light transfer for quantifying the light fluence of Cherenkov radiation from any radiation source, spatial distribution, and optical properties, and provide example estimates for a number of relevant radionuclides and radiotherapy beams using a recently developed and validated Monte Carlo package for the simulation of radiation-induced light transport in biological media .…”

Section: Introductionmentioning

confidence: 99%

Cherenkov radiation fluence estimates in tissue for molecular imaging and therapy applications

et al. 2016

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Cherenkov radiation has recently emerged as an interesting phenomenon for a number of applications in the biomedical sciences. Its unique properties, including broadband emission spectrum, spectral weight in the ultraviolet and blue wavebands, and local generation of light within a given tissue, have made it an attractive new source of light within tissue for molecular imaging and phototherapy applications. While several studies have investigated the total Cherenkov light yield from radionuclides in units of [photons/decay], further consideration of the light propagation in tissue is necessary to fully consider the utility of this signal in vivo. Therefore, to help further guide the development of this novel field, quantitative estimates of the light fluence rate of Cherenkov radiation from both radionuclides and radiotherapy beams in a biological tissue are presented for the first time. Using Monte Carlo simulations, these values were found to be on the order of 0.01 -1 nW/cm 2 per MBq/g for radionuclides, and 1 -100 µW/cm 2 per Gy/sec for external radiotherapy beams, dependent on the given waveband, optical properties, and radiation source. For phototherapy applications, the total light fluence was found to be on the order of nJ/cm 2 for radionuclides, and mJ/cm 2 for radiotherapy beams. The results indicate that diagnostic potential is reasonable for Cherenkov excitation of molecular probes, but phototherapy may remain elusive at such exceedingly low fluence values. The simulation tools of this study are available upon request.

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In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light

Cited by 80 publications

References 22 publications

In vivo Cerenkov luminescence imaging: a new tool for molecular imaging

In vivo Cerenkov luminescence imaging: a new tool for molecular imaging

Optimizing in vivo small animal Cerenkov luminescence imaging

Cherenkov radiation fluence estimates in tissue for molecular imaging and therapy applications

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