In vivo preclinical cancer and tissue engineering applications of absolute oxygen imaging using pulse EPR

Epel, Boris; Kotecha, Mrignayani; Halpern, Howard J.

doi:10.1016/j.jmr.2017.04.017

Cited by 40 publications

(35 citation statements)

References 55 publications

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“…However, EPR has probably gained its widest biological potential yet as EPR Imaging (EPRI) within the context of biomedical research 3. With EPRI, imaging of tissue O 2 partial pressure ( p O 2 )4 and reactive oxygen species (ROS) microenvironments is possible and ideal in the sense that it is both non-invasive and quantitative so that it can be carried out as an in vivo technique (Fig. 3C).…”

Section: Spectroscopy Imagingmentioning

confidence: 99%

“…Unfortunately, EPRI does not provide anatomical information and suffers from poor spatial resolution (1 mm), relative to other imaging techniques. Even with this limited spatial resolution, EPRI has been used to map redox status in tumours, which can been used to predict resistance and reoccurrence to radiation therapies 4,7. When combined with other metal imaging modalities, EPRI can provide an important chemical context for tissue level metal redox processes involving unpaired electrons.…”

Section: Spectroscopy Imagingmentioning

confidence: 99%

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Across the spectrum: integrating multidimensional metal analytics forin situmetallomic imaging

Stewart

2019

Metallomics

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Section: Spectroscopy Imagingmentioning

confidence: 99%

Section: Spectroscopy Imagingmentioning

confidence: 99%

Across the spectrum: integrating multidimensional metal analytics forin situmetallomic imaging

Stewart

2019

Metallomics

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“…In addition, PET scans result in radiation exposure, limiting the frequency with which they can be safely used. Quantitative imaging of hypoxia using electron paramagnetic resonance imaging is under development, but will require advances in readout technologies and the use of probe compounds that are not currently approved for use in humans [8]. Direct measurement of oxygen within tumours is possible using the Eppendorf polarographic needle electrode.…”

Section: Introductionmentioning

confidence: 99%

Real-time measurement of tumour hypoxia using an implantable microfabricated oxygen sensor

Marland¹,

Gray²,

Dunare³

et al. 2020

Preprint

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Hypoxia commonly occurs within tumours and is a major cause of radiotherapy resistance. Clinical outcomes could be improved by locating and selectively increasing the dose delivered to hypoxic regions. Here we describe a miniature implantable sensor for real-time monitoring of tissue oxygenation that could enable this novel treatment approach to be implemented. The sensor uses a solid-state electrochemical cell that was microfabricated at wafer level on a silicon substrate, and includes an integrated reference electrode and electrolyte membrane. It gave a linear response to oxygen concentration, and was unaffected by sterilisation and irradiation, but showed susceptibility to biofouling. Oxygen selectivity was also evaluated against various clinically relevant electroactive compounds. We investigated its robustness and functionality under realistic clinical conditions using a sheep model of lung cancer. The sensor remained functional following CT-guided tumour implantation, and was sufficiently sensitive to track acute changes in oxygenation within tumour tissue.

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“…As such, techniques for the visualization of the distribution of partial pressure of oxygen in tumors are of significant interest in pre-clinical and clinical research. To date, several non-invasive methods for oxygen-sensitive imaging have been reported, including near-infrared spectroscopy (NIRS) [2], fluorescence/phosphorescence imaging [3], positron emission tomography (PET) using hypoxia radiotracers [4], 19 F-magnetic resonance imaging (MRI) [5], blood oxygen level-dependent MRI [6], and electron paramagnetic resonance (EPR) imaging [7,8]. Of these imaging modalities, EPR is one of the few methods that enable quantitative visualization of the partial pressure of oxygen in tumors.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous T2* mapping of 14N- and 15N-labeled dicarboxy-PROXYLs using CW-EPR-based single-point imaging

Yokoyama

Taguchi

Kubota

et al. 2019

Journal of Magnetic Resonance

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This article reports a method of simultaneous T 2 * mapping of 14 N-and 15 N-labeled dicarboxy-PROXYLs using 750-MHz continuous-wave electron paramagnetic resonance (CW-EPR) imaging. To separate the spectra of 14 N-and 15 N-labeled dicarboxy-PROXYLs under magnetic field gradients, an optimization problem for spectral projections was formulated with the spatial total variation as a regularization term and solved using a local search based on the gradient descent algorithm. Using the single-point imaging (SPI) method with spectral projections of each radical, simultaneous T 2 * mapping was performed for solution samples. Simultaneous T 2 * mapping enabled visualization of the response of T 2 * values to the level of dissolved oxygen in the solution. Simultaneous T 2 * mapping applied to a mouse tumor model demonstrated the feasibility of the reported method for potential application to in vivo oxygenation imaging.

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In vivo preclinical cancer and tissue engineering applications of absolute oxygen imaging using pulse EPR

Cited by 40 publications

References 55 publications

Across the spectrum: integrating multidimensional metal analytics forin situmetallomic imaging

Across the spectrum: integrating multidimensional metal analytics forin situmetallomic imaging

Real-time measurement of tumour hypoxia using an implantable microfabricated oxygen sensor

Simultaneous T2* mapping of 14N- and 15N-labeled dicarboxy-PROXYLs using CW-EPR-based single-point imaging

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