In Vivo Pressurization of the Zebrafish Embryonic Heart as a Tool to Characterize Tissue Properties During Development

Gendernalik, Alex; Zebhi, Banafsheh; Ahuja, Neha; Garrity, Deborah M.

doi:10.1007/s10439-020-02619-5

Cited by 6 publications

(9 citation statements)

References 41 publications

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“…It has previously been reported from a chick embryo study on epicardial strain during Hamburger‐Hamilton 22 stages 21, 24, 27 and 31 that ventricular wall deformation during systole changes from an isotropic to chamber‐specific anisotropic deformation during development 23 . In another study, the authors studied the mechanical properties of the zebrafish embryonic atrium at 2 dpf to infer their isotropic or transversely isotropic deformation at pressurization 24 . In this work, we monitored the progressive changes in anisotropic wall deformation and their regional differences in the ventricle through cardiac cycles accompanying the development of the embryonic hearts from 3, 4, to 5 dpf.…”

Section: Discussionmentioning

confidence: 99%

“…Using a simplified Laplace law, with measurements of radii of curvature and wall thicknesses, we calculated an elastic stiffness at 1.4, 3.9, and 5.1 kPa for the ventricular wall of the embryonic heart at 3, 4, and 5 dpf. The zebrafish atrium's mechanical properties have been reported to be on the order of 10 kPa immediately after looping 24 . Work on the chick heart has shown that myocardial stiffness increases daily to 1 to 2 kPa during development up to Hamburger‐Hamilton stage 26 25 (equivalent of 5 dpf).…”

Section: Discussionmentioning

confidence: 99%

“…23 In another T A B L E 5 Characteristic intraventricular pressure (IVP) generation (IVP) vs averaged area ratios (AR) from end-diastole (ED) to enddiastole (ES) obtained by fitting pressure measurements (n = 5) vs scaled AR using a fifth order polynomial for each of 3-, 4-, and 5-dpf groups Fitted coefficients study, the authors studied the mechanical properties of the zebrafish embryonic atrium at 2 dpf to infer their isotropic or transversely isotropic deformation at pressurization. 24 In this work, we monitored the progressive changes in anisotropic wall deformation and their regional differences in the ventricle through cardiac cycles accompanying the development of the embryonic hearts from 3, 4, to 5 dpf. These developmental changes contribute to increased ventricular wall stiffness (higher 1/VC) and an increased pumping effectiveness (higher SPI) for the developing heart.…”

Section: Anisotropic Deformation Of Ventricular Myocardium In Develop...mentioning

confidence: 99%

“…The zebrafish atrium's mechanical properties have been reported to be on the order of 10 kPa immediately after looping. 24 Work on the chick heart has shown that myocardial stiffness increases daily to 1 to 2 kPa during development up to Hamburger-Hamilton stage 26 25 (equivalent of 5 dpf). Clearly, the evolutional changes in ventricular mechanical properties through development is an area need additional work.…”

Section: Passive Deformational Responses and Ventricular Wall Stiffne...mentioning

confidence: 99%

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Ventricular anisotropic deformation and contractile function of the developing heart of zebrafish in vivo

Salehin

Teranikar

Lee

et al. 2022

Developmental Dynamics

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Background The developing zebrafish ventricle generates higher intraventricular pressure (IVP) with increasing stroke volume and cardiac output at different developmental stages to meet the metabolic demands of the rapidly growing embryo (Salehin et al. Ann Biomed Eng, 2021;49(9): 2080‐2093). To understand the changing role of the developing embryonic heart, we studied its biomechanical characteristics during in vivo cardiac cycles. By combining changes in wall strains and IVP measurements, we assessed ventricular wall stiffness during diastolic filling and the ensuing systolic IVP‐generation capacity during 3‐, 4‐, and 5‐day post fertilization (dpf). We further examined the anisotropy of wall deformation, in different regions within the ventricle, throughout a complete cardiac cycle. Results We found the ventricular walls grow increasingly stiff during diastolic filling with a corresponding increase in IVP‐generation capacity from 3‐ to 4‐ and 5‐dpf groups. In addition, we found the corresponding increasing level of anisotropic wall deformation through cardiac cycles that favor the latitudinal direction, with the most pronounced found in the equatorial region of the ventricle. Conclusions From 3‐ to 4‐ and 5‐dpf groups, the ventricular wall myocardium undergoes increasing level of anisotropic deformation. This, in combination with the increasing wall stiffness and IVP‐generation capacity, allows the developing heart to effectively pump blood to meet the rapidly growing embryo's needs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Anisotropic Deformation Of Ventricular Myocardium In Develop...mentioning

confidence: 99%

Section: Passive Deformational Responses and Ventricular Wall Stiffne...mentioning

confidence: 99%

See 2 more Smart Citations

Ventricular anisotropic deformation and contractile function of the developing heart of zebrafish in vivo

Salehin

Teranikar

Lee

et al. 2022

Developmental Dynamics

View full text Add to dashboard Cite

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“…Uniaxial/biaxial tensile testing [30] Optical coherence tomography [31,32] Invasive/noninvasive residual stress experiments [33] Epifluorescence/fluoroscopy [34] In vivo pressurization [35] Microscopy [34,36] Optical stretching and optical tweezers [37] Magnetic resonance imaging [18] Finite element modeling (FEM) [3] Echocardiograph [38,39] Cantilever based technologies [29] Confocal/two-photon microscopy [19] Strain energy and Gasser-Ogden-Holzapfel models [40] Scanning electron microscopy [41,42] Cuts [43] Histology [44] Micropipette aspiration with servo-null pressure measurements [45] Digital camera…”

Section: Refmentioning

confidence: 99%

Soft-Tissue Material Properties and Mechanogenetics during Cardiovascular Development

Siddiqui

Dogru

Lashkarinia

et al. 2022

JCDD

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During embryonic development, changes in the cardiovascular microstructure and material properties are essential for an integrated biomechanical understanding. This knowledge also enables realistic predictive computational tools, specifically targeting the formation of congenital heart defects. Material characterization of cardiovascular embryonic tissue at consequent embryonic stages is critical to understand growth, remodeling, and hemodynamic functions. Two biomechanical loading modes, which are wall shear stress and blood pressure, are associated with distinct molecular pathways and govern vascular morphology through microstructural remodeling. Dynamic embryonic tissues have complex signaling networks integrated with mechanical factors such as stress, strain, and stiffness. While the multiscale interplay between the mechanical loading modes and microstructural changes has been studied in animal models, mechanical characterization of early embryonic cardiovascular tissue is challenging due to the miniature sample sizes and active/passive vascular components. Accordingly, this comparative review focuses on the embryonic material characterization of developing cardiovascular systems and attempts to classify it for different species and embryonic timepoints. Key cardiovascular components including the great vessels, ventricles, heart valves, and the umbilical cord arteries are covered. A state-of-the-art review of experimental techniques for embryonic material characterization is provided along with the two novel methods developed to measure the residual and von Mises stress distributions in avian embryonic vessels noninvasively, for the first time in the literature. As attempted in this review, the compilation of embryonic mechanical properties will also contribute to our understanding of the mature cardiovascular system and possibly lead to new microstructural and genetic interventions to correct abnormal development.

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Valveless pumping behavior of the simulated embryonic heart tube as a function of contractile patterns and myocardial stiffness

Sharifi

Gendernalik

Garrity

et al. 2021

Biomech Model Mechanobiol

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In Vivo Pressurization of the Zebrafish Embryonic Heart as a Tool to Characterize Tissue Properties During Development

Cited by 6 publications

References 41 publications

Ventricular anisotropic deformation and contractile function of the developing heart of zebrafish in vivo

Ventricular anisotropic deformation and contractile function of the developing heart of zebrafish in vivo

Soft-Tissue Material Properties and Mechanogenetics during Cardiovascular Development

Valveless pumping behavior of the simulated embryonic heart tube as a function of contractile patterns and myocardial stiffness

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