In vivo production of mineralised tissue pieces for clinical use: a qualitative pilot study using human dental pulp cell

Chan, BaeYong; Wong, Rwk; Rabie, Bakr

doi:10.1016/j.ijom.2011.01.008

Cited by 24 publications

(19 citation statements)

References 35 publications

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“…Our results are consistent with those reported by Kohen et al (2009), who demonstrated that cells grown on MatrigelBD retained all pluripotent stem cells features. Similar results reported by Chan et al (2011) demonstrated the biocompatibility of Puramatrix TM with DPSCs and SHEDs, showing that this hydrogel is compatible and allows cellular proliferation (Drury & Mooney; Chan et al). Here we revealed that MatrigelBD is easy to manipulate, it allows the inclusion of GMSCs, and it incorporates several growth factors that stimulate cellular proliferation, hence creating a suitable environment for the potential use in tissue reparation or regeneration.…”

Section: Discussionsupporting

confidence: 73%

MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation

et al. 2016

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SUMMARY:MatrigelBD is a hydrogel scaffold with three-dimensional intercrossed networks of hydrophilic polymers with high water content. Human gingival tissue might represent a better source of MSCs, allowing these cells to be easily obtained in a relatively non-invasive way. The objective of this study was to evaluate the biocompatibility of MatrigelBD with GMSCs in vitro. Gingival connective tissue samples were obtained from healthy donors. Fresh tissue was minced and cultured during two weeks, after which cells at passage fourth were analyzed for their immune phenotype by flow cytometry. Differentiation into osteogenic, chondrogenic, and adipogenic lineages was induced and evaluated by culture staining. The "construct" was made of MatrigelBD with GMSC. To assess the biocompatibility, an MTT cellular proliferation assay was performed. The differentiation potential of the cells toward the osteogenic, adipogenic, and chondrogenic lineages was analyzed after 21 days of growth in MatrigelBD with induction differentiation media. The MTT analysis showed that MatrigelBD stimulated cell proliferation; the GMSCs maintained the expression of MSC markers. Importantly, the growth of GMSCs within the MatrigelBD did not interfere with the cell differentiation potential. These findings indicate that MatrigelBD is biocompatible with GMSCs, and this matrix improves cell proliferation in vitro.

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Section: Discussionsupporting

confidence: 73%

MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation

et al. 2016

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“…Some investigators reported the successful formation of lamellar bone in vivo by inducing human DPSCs to synthesize bone tissue in vitro and then transplanting it subcutaneously into mice, without needing of a scaffold support as the transplanted fibrous bone was an already formed hard tissue [ 48 , 49 ]. Chan et al used a self-assembling peptide hydrogel scaffold seeded with DPSC to create mineralised bone-like tissue pieces containing blood capillaries [ 50 ]. DPSCs with a mature osteogenic phenotype have been reported to be more responsive to pulsating fluid shear stress than osteogenically immature DPSCs and produce more bone in vivo suggesting that DPSCs with a mature osteogenic phenotype might be preferable for bone tissue engineering, because they might be able to perform mature bone cell-specific functions during bone adaptation to mechanical loading in vivo [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Today Prospects for Tissue Engineering Therapeutic Approach in Dentistry

Bossù

Pacifici

Carbone

et al. 2014

The Scientific World Journal

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In dental practice there is an increasing need for predictable therapeutic protocols able to regenerate tissues that, due to inflammatory or traumatic events, may suffer from loss of their function. One of the topics arising major interest in the research applied to regenerative medicine is represented by tissue engineering and, in particular, by stem cells. The study of stem cells in dentistry over the years has shown an exponential increase in literature. Adult mesenchymal stem cells have recently been isolated and characterized from tooth-related tissues and they might represent, in the near future, a new gold standard in the regeneration of all oral tissues. The aim of our review is to provide an overview on the topic reporting the current knowledge for each class of dental stem cells and to identify their potential clinical applications as therapeutic tool in various branches of dentistry.

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“…PLLA/differentiated hAFSC98 PLLA/n‐HA;99 PEOT/PBT/calcium phosphate/MSC;100 PCL/gelatin/n‐HA/differentiated DPSC;101 and Self‐assembling PA/differentiated DPSC102…”

Section: Medicinementioning

confidence: 99%

“…Thus, recently, stem cells have been usually incorporated into scaffolds to speed up tissue regeneration. The incorporation of MSCs,100, 103, 108, 110, 127, 128 amniotic fluid‐derived stem cells (AFSCs)98 and dental pulp stem cell (DPSCs),101, 102 etc. into nanofibous scaffolds helped to accelerate bone regeneration.…”

Section: Medicinementioning

confidence: 99%

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Biological, Chemical, and Electronic Applications of Nanofibers

Nguyen

Chen

Elumalai

et al. 2012

Macro Materials & Eng

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With their high‐surface‐to‐volume ratio, nanofibers have been postulated to increase interactions between nanofibrous materials and targeted substrates, which are helpful to overcome many obstacles and enhance the efficiency in a diverse number of applications. Over the past decade, many studies have been published on the fabrication of nanofibers and their applications in various fields. In this review, novel biological, chemical, and electrical characteristics of nanofibers as well as their recent status and achievements in medicine, chemistry, and electronics are analyzed. It is found that nanofibers can induce fast regeneration of many tissues/organs in medical applications and improve the efficiency of many chemical and electronics applications.

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In vivo production of mineralised tissue pieces for clinical use: a qualitative pilot study using human dental pulp cell

Cited by 24 publications

References 35 publications

MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation

MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation

Today Prospects for Tissue Engineering Therapeutic Approach in Dentistry

Biological, Chemical, and Electronic Applications of Nanofibers

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