In vivo protein corona patterns of lipid nanoparticles

Abstract

In vitro and in vivo biological identity of nanoparticles are substantially different.

Albumin-driven disassembly of lipidic nanoparticles: the specific case of the squalene-adenosine nanodrug

“…Indeed, contrary to what has been previously observed (e.g. with lipid nanoparticles 74 or polystyrene spheres 53 ) our cryo-TEM images do not show any layer of adsorbed proteins onto the SQAd nanoparticles surface in spite of the large number of free BSA surrounding them. However, the small decrease in free BSA in solution inferred from SANS fittings (See Table 2) and the observed colloidal stabilization might indirectly suggest that some proteins are partially adsorbed on the surface of the SQAd nanoparticles, thus slightly modifying their surface charge.…”

In situanalysis of liposome hard and soft protein corona structure and composition in a single label-free workflow

“…Recent results by Amici et al suggest that in vitro conditions exacerbate the binding of the three fibrinogen chains to liposomes compared to in vivo conditions, and the presence of apolipoproteins is increased. 60 Next, we performed a relative enrichment analysis using only those proteins with abundance values in at least three biological replicates (139 in total). Interestingly, F2 repeatedly produced a distinct SC2 composition, with protein annotations that were found depleted in the other SCs and containing "HC-type proteins" with 52 proteoforms in common with HCs and only 18 distinct for SC2 (Fig.…”

“…Progress has been made in the proteomic characterization of the corona, often by comparing different NPs as a function of time in a variety of physiological fluids, 9,10 and more recently by contrasting in vitro and in vivo conditions in both humans and experimental animals. [11][12][13][14] Several environmental corona-altering variables have been identified, e.g. dynamic flow conditions, 17 temperature, 18 and duration of exposure.…”

Lipid Corona Formation from Nanoparticle Interactions with Bilayers

“…The propensity of biological species to form coronas around nanoparticles [1][2][3][4][5] has been used for preparing engineered nanomaterials that can be distributed in biological systems with some control. [6][7][8][9][10][11] Although protein coronas in particular have been studied extensively, 1-3,6,7 our understanding of lipid coronas is now just beginning to emerge, [12][13][14][15] especially of those formed upon unintended nanoparticle contacts with living cells. The protein and lipid corona formation mechanisms appear to differ substantially, as ''hard'' and ''soft'' coronas, 16 typical for the former, have not been described for the latter.…”