In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea

Andrade, A. E.; Silva, Luciano; Pereira, Jackeline L.; Noronha, Eliane Ferreira; Reis, Fábio Bueno dos; Bloch, Carlos; Santos, Marise Fonseca dos; Domont, Gilberto B.; Franco, Octávio L.; Mehta, Ângela

doi:10.1111/j.1574-6968.2008.01090.x

Cited by 46 publications

(34 citation statements)

References 35 publications

(39 reference statements)

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“…6 Infection of Arabidopsis suspension cells with Pseudomonas syringae resulted in the secretion collection of 45 Arabidopsis secreted proteins, 7 and similar sized collections of proteins were obtained from Xanthamonas campestris infection of Brassica oleracea although in this case most of the proteins identified were of bacterial origin. 8 The proteome of tomato fruits ( Lycopersicon esculentum ) infected with TMV identified 16 proteins. including several pathogenesis-related (PR) proteins and antioxidant enzymes found that may be part of the plant resistance response to viral infection.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Ripening Tomato Fruit Infected by Botrytis cinerea

et al. 2012

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Botrytis cinerea, a model necrotrophic fungal pathogen that causes gray mold as it infects different organs on more than 200 plant species, is a significant contributor to postharvest rot in fresh fruit and vegetables, including tomatoes. By describing host and pathogen proteomes simultaneously in infected tissues, the plant proteins that provide resistance and allow susceptibility and the pathogen proteins that promote colonization and facilitate quiescence can be identified. This study characterizes fruit and fungal proteins solubilized in the B. cinerea−tomato interaction using shotgun proteomics. Mature green, red ripe wild type and ripening inhibited (rin) mutant tomato fruit were infected with B. cinerea B05.10, and the fruit and fungal proteomes were identified concurrently 3 days postinfection. One hundred eighty-six tomato proteins were identified in common among red ripe and red ripe-equivalent ripening inhibited (rin) mutant tomato fruit infected by B. cinerea. However, the limited infections by B. cinerea of mature green wild type fruit resulted in 25 and 33% fewer defense-related tomato proteins than in red and rin fruit, respectively. In contrast, the ripening stage of genotype of the fruit infected did not affect the secreted proteomes of B. cinerea. The composition of the collected proteins populations and the putative functions of the identified proteins argue for their role in plant−pathogen interactions.

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Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Ripening Tomato Fruit Infected by Botrytis cinerea

et al. 2012

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“…campestris in association with B. oleracea and Pseudomonas savastanoi pv. savastanoi resulted in comprehensive expression analysis including stress and metabolic proteins (Andrade et al, 2008; Campos et al, 2009). Increased levels of proteins involved in xanthan biosynthesis, stress response, and the metabolism were induced in X. campestris in planta conditions compared with in vitro grown cells (Andrade et al, 2008).…”

Section: Proteome Analyses Of Plant Associated Bacteriamentioning

confidence: 99%

“…savastanoi resulted in comprehensive expression analysis including stress and metabolic proteins (Andrade et al, 2008; Campos et al, 2009). Increased levels of proteins involved in xanthan biosynthesis, stress response, and the metabolism were induced in X. campestris in planta conditions compared with in vitro grown cells (Andrade et al, 2008). Chaperonin is reported to be involved in stress responses and EF, which acts as an important PTI in the plants, is the key component of the translational machinery of bacteria.…”

Section: Proteome Analyses Of Plant Associated Bacteriamentioning

confidence: 99%

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Plant-bacterium interactions analyzed by proteomics

et al. 2013

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The evolution of the plant immune response has resulted in a highly effective defense system that is able to resist potential attack by microbial pathogens. The primary immune response is referred to as pathogen associated molecular pattern (PAMP) triggered immunity and has evolved to recognize common features of microbial pathogens. In response to the delivery of pathogen effector proteins, plants acquired R proteins to fight against pathogen attack. R-dependent defense response is important in understanding the biochemical and cellular mechanisms and underlying these interactions will enable molecular and transgenic approaches for crops with increased biotic resistance. Proteomic analyses are particularly useful for understanding the mechanisms of host plant against the pathogen attack. Recent advances in the field of proteome analyses have initiated a new research area, i.e., the analysis of more complex microbial communities and their interaction with plant. Such areas hold great potential to elucidate, not only the interactions between bacteria and their host plants, but also of bacteria-bacteria interactions between different bacterial taxa, symbiotic, pathogenic bacteria, and commensal bacteria. During biotic stress, plant hormonal signaling pathways prioritizes defense over other cellular functions. Some plant pathogens take advantage of hormone dependent regulatory system by mimicking hormones that interfere with host immune responses to promote virulence (vir). In this review, it is discussed the cross talk that plays important role in response to pathogens attack with different infection strategies using proteomic approaches.

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“…Using this protocol and the corresponding proteomics approaches, Andrade et al . () analysed the in vivo proteome of X. campestris pv. campestris in different cultivars of infected Brassica oleracea .…”

Section: Introductionmentioning

confidence: 97%

Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence

Wang

Zhou

et al. 2016

Molecular Plant Pathology

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The identification of phytopathogen proteins that are differentially expressed during the course of the establishment of an infection is important to better understand the infection process. In vitro approaches, using plant extracts added to culture medium, have been used to identify such proteins, but the biological relevance of these findings for in planta infection are often uncertain until confirmed by in vivo studies. Here, we compared the proteins of Pectobacterium carotovorum ssp. carotovorum strain PccS1 differentially expressed in Luria-Bertani medium supplemented with extracts of the ornamental plant Zantedeschia elliotiana cultivar 'Black Magic' (in vitro) and in plant tissues (in vivo) by two-dimensional electrophoresis coupled with mass spectrometry. A total of 53 differentially expressed proteins (>1.5-fold) were identified (up-regulated or down-regulated in vitro, in vivo or both). Proteins that exhibited increased expression in vivo but not in vitro, or in both conditions, were identified, and deletions were made in a number of genes encoding these proteins, four of which (clpP, mreB, flgK and eda) led to a loss of virulence on Z. elliotiana, although clpP and mreB were later also shown to be reduced in growth in rich and minimal media. Although clpP, flgK and mreB have previously been reported as playing a role in virulence in plants, this is the first report of such a role for eda, which encodes 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, a key enzyme in Entner-Doudoroff metabolism. The results highlight the value of undertaking in vivo as well as in vitro approaches for the identification of new bacterial virulence factors.

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In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea

Cited by 46 publications

References 35 publications

Proteomic Analysis of Ripening Tomato Fruit Infected by Botrytis cinerea

Proteomic Analysis of Ripening Tomato Fruit Infected by Botrytis cinerea

Plant-bacterium interactions analyzed by proteomics

Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence

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