In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography

Taylor, Olivia; Laeken, Nick Van; Vos, Filip De; Polis, Ingeborgh; Bosmans, Tim; Goethals, Ingeborg; Achten, Rik; Dobbeleir, André; Vandermeulen, Eva; Baeken, Chris; Saunders, Jimmy; Peremans, Kathelijne

doi:10.1186/s12917-015-0622-3

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…At baseline levels, the regional distribution was consistent with the characteristic distribution of SERT in Beagles, with high SERT-availabilities in the colliculi, the thalamus and the brainstem region containing the raphe nuclei, intermediate availabilities in the basal ganglia and hippocampus, and negligible availabilities in the cerebellar cortex [ 17 ]. After escitalopram treatment, a strong decline was observed in all ROIs in comparison to the cerebellar cortex, whose shape was unchanged confirming the lack of specific binding in the latter.…”

Section: Resultsmentioning

confidence: 52%

Estimation of the optimal dosing regimen of escitalopram in dogs: A dose occupancy study with [11C]DASB

et al. 2017

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Although the favourable characteristics of escitalopram as being the most selective serotonin reuptake inhibitor and having an increased therapeutic efficacy via binding on an additional allosteric binding site of the serotonin transporter, its dosing regimen has not yet been optimized for its use in dogs. This study aimed to estimate the optimal dosing frequency and the required dose for achieving 80% occupancy of the serotonin transporters in the basal ganglia. The dosing frequency was investigated by determining the elimination half-life after a four day oral pre-treatment period with 0.83 mg/kg escitalopram (3 administrations/day) and a subsequent i.v. injection 0.83 mg/kg. Blood samples were taken up to 12 hours after i.v. injection and the concentration of escitalopram in plasma was analysed via LC-MSMS. The dose-occupancy relationship was then determined by performing two PET scans in five adult beagles: a baseline PET scan and a second scan after steady state conditions were achieved following oral treatment with a specific dose of escitalopram ranging from 0.5 to 2.5 mg/kg/day. As the elimination half-life was determined to be 6.7 hours a dosing frequency of three administrations a day was proposed for the second part of the study. Further it was opted for a treatment period of four days, which well exceeded the minimum period to achieve steady state conditions. The optimal dosing regimen to achieve 80% occupancy in the basal ganglia and elicit a therapeutic effect, was calculated to be 1.85 mg/kg/day, divided over three administrations. Under several circumstances, such as insufficient response to other SSRIs, concurrent drug intake or in research studies focused on SERT, the use of escitalopram can be preferred over the use of the already for veterinary use registered fluoxetine, however, in case of long-term treatment with escitalopram, regularly cardiac screening is recommended.

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Section: Resultsmentioning

confidence: 52%

Estimation of the optimal dosing regimen of escitalopram in dogs: A dose occupancy study with [11C]DASB

et al. 2017

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“…N-methylation of the precursor N-desmethyl-DASB (50 μg, ABX, Radeberg, Germany), with a [ 11 C]methyl triflate, was performed in order to synthesize the SERT ligand [ 11 C]DASB. Radiochemical purity of more than 99% was achieved [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, nuclear brain imaging studies have demonstrated parallels in alterations in neurotransmitter systems and neuronal function (brain perfusion and metabolism) in humans [ 23 , 24 , 25 , 26 ] and dogs [ 27 , 28 , 29 ] suffering from similar neuropsychiatric disorders. Of interest, regions with high SERT density are also conserved between different species such as rodents [ 30 ], pigs [ 31 ], cats [ 32 ], non-human primates [ 33 ], and also dogs [ 34 ]. Given that rTMS protocols used in the treatment of human depression can be similarly applied in canine species [ 35 ], examining neurobiological effects of novel rTMS protocols in the canine model may add to the fine-tuning of such treatments in humans.…”

Section: Introductionmentioning

confidence: 99%

Accelerated HF-rTMS Modifies SERT Availability in the Subgenual Anterior Cingulate Cortex: A Canine [11C]DASB Study on the Serotonergic System

Kappen

Peremans

et al. 2022

JCM

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Repetitive transcranial magnetic stimulation (rTMS) is thought to partly exert its antidepressant action through the serotonergic system. Accelerated rTMS may have the potential to result in similar but faster onset of clinical improvement compared to the classical daily rTMS protocols, but given that delayed clinical responses have been reported, the neurobiological effects of accelerated paradigms remain to be elucidated including on this neurotransmitter system. This sham-controlled study aimed to evaluate the effects of accelerated high frequency rTMS (aHF-rTMS) over the left frontal cortex on the serotonin transporter (SERT) in healthy beagle dogs. A total of twenty-two dogs were randomly divided into three unequal groups: five active stimulation sessions (five sessions in one day, n = 10), 20 active stimulation sessions (five sessions/day for four days, n = 8), and 20 sham stimulation sessions (five sessions/day for four days, n = 4). The SERT binding index (BI) was obtained at baseline, 24 h post stimulation protocol, one month, and three months post stimulation by a [11C]DASB PET scan. It was found that one day of active aHF-rTMS (five sessions) did not result in significant SERT BI changes at any time point. For the 20 sessions of active aHF-rTMS, one month after stimulation the SERT BI attenuated in the sgACC. No significant SERT BI changes were found after 20 sessions of sham aHF-rTMS. A total of four days of active aHF-rTMS modified sgACC SERT BI one month post-stimulation, explaining to some extent the delayed clinical effects of accelerated rTMS paradigms found in human psychopathologies.

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“…Positron Emission Tomography (PET) is increasingly being used as an imaging modality for clinical and research applications in veterinary medicine. It plays an important role in the diagnosis, staging, image-guided treatment planning, and monitoring of malignant diseases, 64 detecting the margin of oral neoplasia, in particular for tumors arising from highly vascularized tissue, such as the lingual and laryngeal areas, and assessment of inflammation and pain, 65 and diagnosis of cognitive dysfunction syndrome, 66 arterial sampling-based kinetic analysis, 67 diagnosis and tracking of primary and metastatic tumors, to study the normal physiology of glucose uptake, metabolism, and muscle activity during and after exercise, evaluation of patients presenting with indistinct or intermittent clinical signs of musculoskeletal inflammation or injury. 68 …”

Section: Veterinary Nuclear Medicine Imagingmentioning

confidence: 99%

Application of Advanced Imaging Modalities in Veterinary Medicine: A Review

Yitbarek¹,

Dagnaw²

2022

VMRR

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Veterinary anatomy has traditionally relied on detailed dissections to produce anatomical illustrations, but modern imaging modalities, now represent an enormous resource that allows for fast non-invasive visualizations in living animals for clinical and research purposes. In this review, advanced anatomical imaging modalities and their applications, safety issues, challenges, and future prospects of the techniques commonly employed for animal imaging would be highlighted. The quality of diagnostic imaging equipment in veterinary practice has greatly improved. Recent advances made in veterinary advanced imaging specifically about cross-sectional modalities (CT and MRI), nuclear medicine (PET, SPECT), and dual imaging modalities (PET/CT, PET/MR, and SPECT/CT) have become widely available, leading to greater demands and expectations from veterinary clients. These modalities allow for the creation of three-dimensional representations that can be of considerable value in the dissemination of clinical diagnosis and anatomical studies. Despite, the modern imaging modalities well established in developed countries across the globe, it is yet to remain in its infancy stage in veterinary practice in developing countries due to heavy initial investment and maintenance costs, lack of expert interpretation, a requirement of specialized technical staff and need of adjustable machines to accommodate the different range of animal sizes. Therefore, veterinarians should take advantage of these imaging techniques in designing future experiments by considering the availability of these varied imaging modalities and the creation of three-dimensional graphical representations of internal structures.

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In vivo quantification of the [11C]DASB binding in the normal canine brain using positron emission tomography

Cited by 4 publications

References 40 publications

Estimation of the optimal dosing regimen of escitalopram in dogs: A dose occupancy study with [11C]DASB

Estimation of the optimal dosing regimen of escitalopram in dogs: A dose occupancy study with [11C]DASB

Accelerated HF-rTMS Modifies SERT Availability in the Subgenual Anterior Cingulate Cortex: A Canine [11C]DASB Study on the Serotonergic System

Application of Advanced Imaging Modalities in Veterinary Medicine: A Review

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