In vivo real-time imaging of the liver with confocal endomicroscopy permits visualization of the temporospatial patterns of hepatocyte apoptosis

Goetz, Martin; Ansems, Jacqueline V.; Galle, Peter R.; Schuchmann, Marcus; Kießlich, Ralf

doi:10.1152/ajpgi.00175.2011

Cited by 23 publications

(14 citation statements)

References 44 publications

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“…Accordingly, intravital microscopy has been applied to a wide range of studies of the liver. Intravital microscopy has been almost routinely used to characterize microvascular flow in the liver, [11][12][13] but has also been used to assay hepatocyte cell death [14][15][16][17][18][19][20] apoptosis, 21 inflammatory cell infiltration, 22-24 microvascular permeability, [25][26][27] mitochondrial function, 14,15,[17][18][19]28,29 steatosis, 30,31 tumor angiogenesis 32 and fibrosis. 33 Fluorescent transport substrates cultured cell systems, it is more difficult to achieve in intravital microscopy, especially in the liver, whose proximity to the diaphragm makes it particularly susceptible to respiration-induced motion.…”

Section: Resultsmentioning

confidence: 99%

Quantitative intravital microscopy of hepatic transport

et al. 2012

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Section: Resultsmentioning

confidence: 99%

Quantitative intravital microscopy of hepatic transport

et al. 2012

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“…The analysis was performed twice separately by the same investigator (PH) using a semiquantitative histology score (S0-S4) based on hepatocyte swelling, shrinkage, loss of integrity of cellular and nuclear membranes, or nuclear alterations, as described previously [24, 25]. …”

Section: Methodsmentioning

confidence: 99%

Targeting Mitochondrial Dysfunction with L-Alpha Glycerylphosphorylcholine

et al. 2016

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BackgroundWe hypothesized that L-alpha-glycerylphosphorylcholine (GPC), a deacylatedphosphatidylcholine derivative, can influence the mitochondrial respiratory activity and in this way, may exert tissue protective effects.MethodsRat liver mitochondria were examined with high-resolution respirometry to analyze the effects of GPC on the electron transport chain in normoxic and anoxic conditions. Besides, Sprague-Dawley rats were subjected to sham operation or standardized liver ischemia-reperfusion (IR), with or without GPC administration. The reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG), the tissue myeloperoxidase, xanthine oxidoreductase and NADPH oxidases activities were measured. Tissue malondialdehyde and nitrite/nitrate formation, together with blood superoxide and hydrogen-peroxide production were assessed.ResultsGPC increased the efficacy of complex I-linked mitochondrial oxygen consumption, with significantly lower in vitro leak respiration. Mechanistically, liver IR injury was accompanied by deteriorated mitochondrial respiration and enhanced ROS production and, as a consequence, by significantly increased inflammatory enzyme activities. GPC administration decreased the inflammatory activation in line with the reduced oxidative and nitrosative stress markers.ConclusionGPC, by preserving the mitochondrial complex I function respiration, reduced the biochemical signs of oxidative stress after an IR episode. This suggests that GPC is a mitochondria-targeted compound that indirectly suppresses the activity of major intracellular superoxide-generating enzymes.

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“…For example, imaging of apoptosis in the murine liver has established the microscopic features of cell death in vivo, which includes initial cell swelling (rather than shrinkage, as previously reported). 49 Such eCLE imaging of epithelial apoptosis after co-staining with acriflavine was able to predict graft-versus-host disease in patients after bone marrow transplantation in real time, resulting in im mediate therapy of this th reatening condition. 50 The epithelial lining of the gut is punctuated by gaps after single cell shedding, which are usually sealed to keep barrier function intact as a part of normal tissue regeneration.…”

Section: Translational Science: Functional Imagingmentioning

confidence: 99%

Microscopic imaging in endoscopy: endomicroscopy and endocytoscopy

Goetz

Malek

Kießlich

2013

Nat Rev Gastroenterol Hepatol

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Performing real-time microscopy has been a vision of endoscopists since the very early phases of gastrointestinal endoscopy. Confocal endomicroscopy, an adaption of confocal laser scanning microscopy, and endocytoscopy, an adaption of white-light microscopy, have been introduced into the endoscopic armamentarium in the past decade. Both techniques yield on-site histological information. Multiple trials have demonstrated the ability of gastroenterologists to obtain and interpret microscopic images from the upper and lower gastrointestinal tract, and also the hepatobiliary-pancreatic system, during endoscopy. Such microscopic information has been successfully used in expert hands to minimize sampling error by 'smart', microscopically targeted biopsies and to guide endoscopic interventions. However, endomicroscopy is also unique in its ability to dynamically visualize cellular processes in their native environment free of artefacts. This ability enables fundamental insights into mechanisms of human diseases in clinical and translational science.

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In vivo real-time imaging of the liver with confocal endomicroscopy permits visualization of the temporospatial patterns of hepatocyte apoptosis

Abstract: In vivo real-time imaging of the liver with confocal endomicroscopy permits visualization of the temporospatial patterns of hepatocyte apoptosis

Cited by 23 publications

References 44 publications

Quantitative intravital microscopy of hepatic transport

Quantitative intravital microscopy of hepatic transport

Targeting Mitochondrial Dysfunction with L-Alpha Glycerylphosphorylcholine

Microscopic imaging in endoscopy: endomicroscopy and endocytoscopy

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