In vivo red cell destruction by anti‐Lu6

Abstract

An example is presented of an IgG1, anti-Lu6, that reacted by indirect antiglobulin test and was capable of destroying antigen-positive red cells in vivo. Two methods for the measurement of red cell survival, 51Cr labeling and flow cytometry, gave the same result: 20 percent of the test dose of Lu:6 red cells was destroyed in the first hour after injection and 80 percent in the first 24 hours. The clinical relevance of the antibody was correctly predicted by an in vitro monocyte monolayer assay. The finding that this example of anti-Lu6 was clinically significant should not be taken to mean that all antibodies directed against high-incidence Lutheran and Lutheran system-related antigens will behave similarly. When such antibodies are encountered, in vivo and/or in vitro studies to assess their clinical significance are necessary before rare blood is used for transfusion.

Applications of flow cytofluorometry to red blood cell immunology

“…Investigators at the New York Blood Center used the opposite approach, of detecting an antigen present on the recipient but absent from the transfused RBCs (76,(94)(95)(96). It is relatively easy to follow the survival of RBCs when a unit of RBCs has been transfused (e.g., in a delayed hemolytic transfusion reaction), but it is not so easy if one wants to accurately follow the survival of 0.5-1 ml of RBCs, as is used with 51 Crlabeled RBCs.…”

Flow-Cytometric Determination of Survival Time and 24-Hour Recovery of Transfused Red Blood Cells

“…Therefore, we were looking for a FC method that works without pretransfusional labeling. ble RBCC were not available [16,[19][20][21][22][23][24]. Recently, we also published a case of AIHA in which we already used FC to determine survival of transfused and autologous RBCs [25].…”

Significance of Anti-LU8 in Pregnancy

“…Although 18 antigens have been assigned to this blood group system, little is known regarding the clinical significance of Luth eran antibodies other than anti-LUl and anti-LU2 [2], Both anti-LUl and anti-LU2 have been implicated in mild hae molytic disease of the newborn (HDN) and anti-LU2 has been associated with mild transfusion reactions [3], Of the other Lutheran antibodies anti-LU3, anti-LU12 and anti-LU14 have been reported in pregnant women with no evidence of causing HDN [2,3]. In vivo destruction of red blood cells by anti-LU6 [4] and anti-LU8 [5] following transfusion has been reported but there are no reports in the literature on the significance of anti-LU8 in pregnancy.…”